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DH5α Competent Cells
Thermo Scientific DH5α Competent Cells are high efficiency, chemically competent E. coli cells ideal for construction of gene banks orRead more
| Catalog Number | Quantity |
|---|---|
| EC0112 | 10 x 100 μL |
Catalog number EC0112
Price (EUR)
137,00
Special offer
Online exclusive
Ends: 21-Jul-2025
149,00Save 12,00 (8%)
Each
In stock
Quantity:
10 x 100 μL
Price (EUR)
137,00
Special offer
Online exclusive
Ends: 21-Jul-2025
149,00Save 12,00 (8%)
Each
Thermo Scientific DH5α Competent Cells are high efficiency, chemically competent E. coli cells ideal for construction of gene banks or generation of cDNA libraries using plasmid-derived vectors. The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gal. RecA1 and endA1 mutations in DH5α cells improve insert stability and quality of the extracted plasmid DNA.
• High transformation efficiency: >1x109 cfu/μg pUC19 DNA
• Suitable for routine and high-throughput cloning applications
• Genetic markers that allow for blue/white colony screening
• Convenient two reactions per vial packaging
• S.O.C. Outgrowth medium included
Applications
DH5α Competent Cells are suitable for variety of applications requiring high transformation efficiency:
• Efficient DNA cloning derived from PCR, cDNA-generation reactions
• recA1 marker facilitates working with difficult to transform DNA
• Ideal for generation of cDNA libraries using plasmid-derived vectors
• Site-directed mutagenesis
Genotype
F– φ80lacZΔ M15 Δ (lacZYA-argF) U169 recA1 endA1 hsdR17 (rK– mK+) phoA supE44 λ- thi–1 gyrA96 relA1
• High transformation efficiency: >1x109 cfu/μg pUC19 DNA
• Suitable for routine and high-throughput cloning applications
• Genetic markers that allow for blue/white colony screening
• Convenient two reactions per vial packaging
• S.O.C. Outgrowth medium included
Applications
DH5α Competent Cells are suitable for variety of applications requiring high transformation efficiency:
• Efficient DNA cloning derived from PCR, cDNA-generation reactions
• recA1 marker facilitates working with difficult to transform DNA
• Ideal for generation of cDNA libraries using plasmid-derived vectors
• Site-directed mutagenesis
Genotype
F– φ80lacZΔ M15 Δ (lacZYA-argF) U169 recA1 endA1 hsdR17 (rK– mK+) phoA supE44 λ- thi–1 gyrA96 relA1
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeCompetent Cell
Contains F' EpisomeLacks F' Episome
EfficiencyHigh Efficiency (>1x10^9 cfu/μg pUC19 DNA)
Cell TypeChemically Competent
LibrariescDNA
Cloning Methylated DNANo
Bacterial or Yeast StrainDH5α
Blue/White ScreeningYes
High-throughput CompatibilityNot High-throughput Compatible
Propagating ccdB VectorsNot for ccdB vector propagation
Shipping ConditionDry Ice
SpeciesE. coli
FormatTube
Product LineThermo Scientific™
For Use With (Application)Cloning
Quantity10 x 100 μL
Unit SizeEach
Contents & Storage
• 10 x DH5α Competent Cells (100 μL)
• pUC19 DNA (50 μL) (10 pg/μL)
• S.O.C. Medium (5 mL)
Store at -80°C.
• pUC19 DNA (50 μL) (10 pg/μL)
• S.O.C. Medium (5 mL)
Store at -80°C.
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Figures
Transformation efficiency of DH5α Competent Cells
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Certificates
Search by lot number or partial lot number
Lot #Certificate TypeDateCatalog Number(s)
3149995ACertificate of AnalysisMar 24, 2025EC0112
3149995Certificate of AnalysisMar 24, 2025EC0112
3088993Certificate of AnalysisJan 17, 2025EC0112
3009446Certificate of AnalysisOct 02, 2024EC0112
2928645ACertificate of AnalysisMay 23, 2024EC0112
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Safety Data Sheets
SDSFrequently asked questions (FAQs)
We do not recommend storing competent E. coli strains in liquid nitrogen as the extreme temperature can be harmful to the cells. Also, the plastic storage vials are not intended to withstand the extreme temperature and may crack or break.
We recommend storing our competent E. coli strains at -80°C. Storage at warmer temperatures, even for a brief period of time, will significantly decrease transformation efficiency.
Ensure that you are using the correct antibiotic at the appropriate concentration. Additionally, make sure the antibiotic is not expired. If colonies exhibit unexpected morphologies, contamination could be a factor. Check your S.O.C. medium and LB growth medium.
Here are a few suggestions:
- Small fragments/linkers are cloning in to your vector instead of your insert; to correct this, gel-purify the insert before ligation
- Ensure that the correct concentrations of X-gal and/or IPTG (if vector contains the lacIq marker) are used
- If spreading X-gal and/or IPTG on your plate, allow sufficient time for the reagents to diffuse into the plate
- Incubate your plate for a longer period to ensure full color development
We recommend trying the following:
- Carry out the puc19 transformation control; this gives you information about the performance of the cells.
- Check plates for expiration and correct media used (LB/agar).
- Confirm that the correct antibiotic and concentration was used.