DMEM/F-12, no glutamine
DMEM/F-12, no glutamine
Gibco™

DMEM/F-12, no glutamine

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM/F-12) is a widely used basal medium for supporting the growth of many differentRead more
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Catalog NumberQuantity
21331046
also known as 21331-046
10 x 500 mL
21331020Promo Image500 mL
Catalog number 21331046
also known as 21331-046
Price (EUR)
301,00
Each
Estimated availability date  Pending  
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Quantity:
10 x 500 mL
Customize this product
Price (EUR)
301,00
Each
Add to cart
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Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM/F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of Gibco™ DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM/F-12 is modified as follows:

With: Phenol Red

Without: L-glutamine, HEPES

The complete formulation is available.

Gibco™ DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids and vitamins with F-12's wide variety of components.

DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5-10% CO2 environment to maintain physiological pH.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Product TypeDulbecco's Modified Eagle Medium (DMEM)
Quantity10 x 500 mL
Shelf Life12 Months
Culture TypeMammalian Cell Culture
FormLiquid
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
With AdditivesHigh Glucose, Phenol Red, Sodium Pyruvate
Without AdditivesNo Glutamine, No HEPES
Unit SizeEach
Contents & Storage
Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
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Certificates

Lot #Certificate TypeDateCatalog Number(s)
3090912Certificate of AnalysisApr 17, 202521331020
2436828Certificate of AnalysisJan 29, 202521331020
2662150Certificate of AnalysisJan 29, 202521331020
2629751Certificate of AnalysisJan 29, 202521331020
2662266Certificate of AnalysisJan 28, 202521331020
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Safety Data Sheets

Frequently asked questions (FAQs)

We do not offer DMEM/F12 medium without folic acid as a standard catalog product. However, our Customs group can make it for you as a custom media. If you are interested, please complete this Custom order inquiry form PDF (https://www.thermofisher.com/content/dam/LifeTech/global/applied-sciences/pdfs/Bioproduction/Gibco-Custom-Media-Buffers-Request-Form.pdf) and email it to custommedia@thermofisher.com. Our Customs group will review your requirements and get back to you within a couple of days with pricing, ordering information, and lead time. After that, you have the choice of proceeding with an “official” order or declining.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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