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Gibco™
RPMI 1640 Medium, no glutamine
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as aRead more
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| Catalog Number | Quantity |
|---|---|
| 31870025 | 500 mL |
| 31870017 | 100 mL |
| 31870074 also known as 31870-074 | 10 x 500 mL |
Catalog number 31870025
Price (EUR)
24,50
Each
-
Quantity:
500 mL
Price (EUR)
24,50
Each
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas. We offer a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This RPMI 1640 is modified as follows:
| With | Without |
| • Phenol Red | • L-glutamine |
| • HEPES |
The complete formulation is available.
Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12 and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.
Customers using Gibco™ RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).
RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell TypeMammalian Cells
Concentration1 X
Culture EnvironmentCO2
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Osmolality260 - 310 mOsm/kg
Product LineGibco™
Product TypeRPMI 1640
Quantity500 mL
Shelf Life12 Months
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
Culture TypeMammalian Cell Culture
FormLiquid
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
With AdditivesPhenol Red, Sodium Bicarbonate
Without AdditivesNo Glutamine, No HEPES, No Sodium Pyruvate
pH7 to 7.4
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.
Media Formulations
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Certificates
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Lot #Certificate TypeDateCatalog Number(s)
3202814Certificate of AnalysisJun 04, 202531870017, 31870025
3090859Certificate of AnalysisApr 24, 202531870017, 31870025
3062441Certificate of AnalysisFeb 14, 202531870017, 31870025
2681778Certificate of AnalysisJan 29, 202531870017, 31870025
2773744Certificate of AnalysisJan 29, 202531870017, 31870025
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Safety Data Sheets
SDSScientific Resources
Product Information
Frequently asked questions (FAQs)
While we know that different wavelengths of light are worse than others for exposure, we would recommend as a best practice to protect the medium from all forms of light exposure including LEDs, as much as possible to ensure optimal performance, as several components within the medium are light sensitive, such as vitamins.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
We have specific gravity information for RPMI 1640 Medium: 1.006 kg/L. In this case, the specific gravity is the same as density as the solvent is water.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Citations & References (1)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Tracing fatty acid metabolism by click chemistry.
Journal:ACS Chem Biol
PubMed ID:22999348
Fatty acids are abundant constituents of all biological systems, and their metabolism is important for normal function at all levels of an organism. Aberrations in fatty acid metabolism are associated with pathological states and have become a focus of current research, particularly due to the interest in metabolic overload diseases.
1 total citations