Opti-MEM™ I Reduced Serum Medium
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Gibco™

Opti-MEM™ I Reduced Serum Medium

Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine SerumRead more
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Catalog NumberQuantity
31985047
also known as 31985-047
500 mL
31985054
also known as 31985-054
10 x 500 mL
Catalog number 31985047
also known as 31985-047
Price (EUR)
51,25
Each
Estimated availability date 21-Jul-2025
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Quantity:
500 mL
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Price (EUR)
51,25
Each
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Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology. Opti-MEM™ I medium is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM™ I medium can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Opti-MEM™ I modifications for a range of cell culture applications.


This Opti-MEM™ I is modified as follows:
With
• L-glutamine
• Phenol Red


The complete formulation is confidential. For more information, please contact Technical Services.

Using Opti-MEM™ I Medium
Opti-MEM™ I Reduced-Serum Medium is a unique medium that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Opti-MEM™ I medium uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Specifications
Cell TypeMammalian Cells
Concentration1 X
Culture EnvironmentCO2
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Osmolality280 - 320 mOsm/kg
Product LineGibco™, Opti-MEM™
Product TypeReduced-Serum Medium
Quantity500 mL
Shelf Life18 Months
Shipping ConditionRoom Temperature
ClassificationAnimal Origin
Culture TypeMammalian Cell Culture
FormLiquid
SterilitySterile-filtered
With AdditivesGlutamine, HEPES, Phenol Red, Sodium Pyruvate, Sodium Bicarbonate
pH7 to 7.4
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 18 months from date of manufacture.
Have questions about this product? Ask our AI assisted search.
Can I aliquot and freeze Opti-MEM I Reduced Serum Medium to conserve it longer? If so, for how long can it be stored?
I noticed that Opti-MEM I Reduced Serum Medium does not contain any serum. Why do you then refer to it as "Reduced Serum Medium"?
Is there any other difference between Opti-MEM I Reduced Serum Medium and Opti-MEM I Reduced Serum Medium, no phenol red besides the presence/absence of phenol red?
Where can I find the osmolality for my lot of Opti-MEM I Reduced Serum Medium?
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Certificates

Lot #Certificate TypeDateCatalog Number(s)
3225302Certificate of AnalysisJun 27, 202531985047, 31985062, 31985070
3150493Certificate of AnalysisJun 22, 202531985047, 31985062, 31985070
3141618Certificate of AnalysisJun 06, 202531985047, 31985062, 31985070
3141616Certificate of AnalysisJun 06, 202531985047, 31985062, 31985070
3137998Certificate of AnalysisJun 04, 202531985047, 31985062, 31985070
5 results displayed, search above for a specific certificate

Safety Data Sheets

Frequently asked questions (FAQs)

The formulation of Opti-MEM I Reduced Serum Medium and that of Opti-MEM I Reduced Serum Medium, no phenol red is the same except for the presence of phenol red in the former medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

We do provide osmolality information on the certificate of analysis. All lots of Opti-MEM I Reduced Serum Medium will meet the osmolality specification of ≥280 to ≤320 mOsm/kg.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Sorry, we don't have data to show that frozen Opti-MEM I Reduced Serum Medium has a longer shelf life. Actually, we recommend storing this medium at 2-8 degrees C in the dark. We do not recommend freezing the medium because it may precipitate.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Opti-MEM I Reduced Serum Medium is a serum-free medium. The reason why it is called “reduced-serum medium” is because you only need to supplement this medium with 1-5% FBS instead of the standard 10% FBS.

Opti-MEM I Reduced Serum Medium can be used as is (without serum) for transfection of cells. However, for long-term cell culture, you need to add serum to the medium to keep cells healthy.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations & References (1)

Citations & References
Abstract
Mechanism for fatty acid
Authors: Kawaguchi Takumi; Osatomi Kiyoshi; Yamashita Hiromi; Kabashima Tsutomu; Uyeda Kosaku;
Journal:J Biol Chem
PubMed ID:11724780
'Carbohydrate-responsive element-binding protein (ChREBP) is a new transcription factor that binds to the carbohydrate-responsive element of the l-type pyruvate kinase gene (l-PK). The aim of this study was to investigate the mechanism by which feeding high fat diets results in decreased activity of ChREBP in the liver (Yamashita, H., Takenoshita, ... More
1 total citations

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