Medio CD Hybridoma
Medio CD Hybridoma
Gibco™

Medio CD Hybridoma

El medio para hibridomas CD es un medio sin proteínas, libre de origen animal y químicamente definido desarrollado específicamente paraMás información
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Número de catálogoCantidad
112790231000 mL
Número de catálogo 11279023
Precio (EUR)
200,00
Each
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Cantidad:
1000 mL
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El medio para hibridomas CD es un medio sin proteínas, libre de origen animal y químicamente definido desarrollado específicamente para respaldar el crecimiento de hibridomas para la producción de anticuerpos. Características del medio para hibridomas CD Gibco™:

• Capacidad para admitir hibridomas y mielomas de suspensión y estacionarios
• Formulados sin L-glutamina para la estabilidad
• Formulación libre de material de origen animal y de proteínas, y definida químicamente

Capacidad para admitir hibridomas y mielomas de suspensión y estacionarios
El medio para hibridomas CD de Gibco™, que es adecuado para el cultivo de líneas celulares de mielomas recombinantes y de hibridomas tradicionales, proporciona altas densidades celulares y una producción elevada de IgG. Los cultivos dependientes del colesterol, como NS0, requieren la adición de concentrado de colesterol lipídico 250X.

Formulado sin L-glutamina para mayor estabilidad
El medio para hibridomas CD Gibco™ requiere la adición de 8 mM de L-glutamina o de suplemento GlutaMAX™ antes de su uso. El suplemento GlutaMAX™ minimiza la acumulación de amoníaco tóxico y mejora la viabilidad y el crecimiento celular en un formato fácil de usar.

Formulación libre de material de origen animal y de proteínas, y definida químicamente
El medio para hibridomas CG Gibco™ no contiene productos de origen animal ni proteínas y se ha definido químicamente, lo que permite una purificación más sencilla de la proteína de interés. Los medios químicamente definidos Gibco™ no contienen proteínas, hidrolisatos ni componentes de composición desconocida.

Uso del producto
Los clientes que utilicen el medio para hibridomas CD Gibco™ en un proceso de fabricación y que tengan una presentación con la FDA, podrán solicitarnos una carta de autorización para hacer referencia a nuestro archivo principal sobre fármacos (DMF) tipo II.

Sistema de fabricación y calidad conforme a las buenas prácticas de fabricación actuales
El medio para hibridomas CD de Gibco™ se fabrica en unas instalaciones conformes con las buenas prácticas de fabricación actuales, situadas en Grand Island, Nueva York (EE. UU.). Las instalaciones se han registrado en la Agencia estadounidense de alimentos y medicamentos (FDA) como fabricante de dispositivos médicos y tienen la certificación según la norma ISO 13485.

Para su uso en investigación o procesos de fabricación posteriores. No apto para uso diagnóstico ni para la administración directa en seres humanos ni en animales.
Especificaciones
Tipo de célulaCélulas de mieloma, Hibridomas
Línea de productosGibco™
Tipo de productoMedio Hybridoma
Cantidad1000 mL
Condiciones de envíoTemperatura ambiente
ClasificaciónSin material de origen animal, definido químicamente y sin proteínas, definido químicamente, sin proteínas
FormularioLíquido
Sin aditivosSin glutamina, Sin rojo fenol
Unit SizeEach
Contenido y almacenamiento
Condiciones de almacenamiento: 2-8 °C. Proteger de la luz
Condiciones de envío: Ambiente
Vida útil: 18 meses a partir de la fecha de fabricación
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Preguntas frecuentes

How do I adapt my cells to serum-free medium?

Cells can be adapted by Sequential or Direct Adaptation. Suggested protocols for each are below, and you can also find more information by searching "Adaptation of Cell Cultures to a Serum-Free Medium" from our website home page.

SEQUENTIAL ADAPTATION
1) Subculture the cells growing in serum-supplemented medium into a 25%:75% mixture of SFM and serum supplemented medium.
2) When the cell density is 5 x 10E5 cells/ml, subculture the cells into a 50%:50% mixture of SFM and serum supplemented medium at a cell density 2.5 x 10E5 to 3 x 10E5 cells/ml.
3) Continue to subculture after the cell density 5 x 10E5 cells/ml in gradually increasing proportions of SFM until the serum is ~0.1% with about 85% cell viability.
4) Subculture the cells into SFM with an innoculum of 2.5 x 10E5 to 3 x 10E5 cells/ml.
5) When the cell density is 1 x 10E6 to 3 x 10E6 cells/ml (4 to 6 days post planting) subculture the cells again.
6) Stock cultures of SFM adapted cells should be subcultured in SFM every 3 to 5 days when the cell density is 1 x 10E6 to 3 x 10E6 cells/ml with 90% viability.

DIRECT ADAPTATION
Some cells can be directly adapted from serum-containing medium to SFM. For direct adaptation, the cell innoculum should be 1.5 x 10E5 to 3 x 10E5 cells/ml.
Cells should be subcultured when the cell density is 1 x 10E6 to 3 x 10E6 cells/ml. Cells are fully adapted to SFM when the cell density is 2 x 10E6 to 4 x 10E6 cells/ml after 4 to 7 days in culture.
Stock cultures of cells adapted to SFM should be subcultured in SFM every 3 to 5 days when the cell density is 1 x 10E6 to 3 x 10E6 cells/ml with 90% viability.

Why is it necessary to gradually adapt the cells to serum-free medium?

Some cells, such as insect cells, are sensitive to changes in their medium. By sequentially adapting cells, the medium is changed with minimal effects on cell growth.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I filter sterilize CD Hybridoma Medium?

CD Hybridoma Medium can be filtered by 0.2 µm filtration.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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Certificados

N.º de loteCertificate TypeDateCatalog Number(s)
3231047Certificate of Analysis04 jul 202511279023
3108022Certificate of Analysis08 jun 202511279023
3138473Certificate of Analysis23 may 202511279023
3093229Certificate of Analysis22 mar 202511279023
2994996Certificate of Analysis05 mar 202511279023
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Notice to Purchaser: The purchase of this product conveys to the purchaser the limited, non- transferable right to use the purchased amount of the product to (a) perform internal research for the sole benefit of the purchaser; (b) manufacture protein (or other biological material) for resale; and (c) perform research or manufacturing services conducted by the purchaser on a fee for service or contract basis for or on behalf of third parties. However, the purchaser may transfer this product, its components, or materials made using this product to a third party (including contract research/manufacturing organizations), provided that each such third party agrees in writing to use such product, components, or materials solely on behalf of the purchaser, and such third party is restricted from further transferring any such product, components, or materials to any individual or entity other than the purchaser. No additional rights are granted. By purchasing this product, the purchaser agrees not to: (1) resell the product in any form; (2) use the product as a therapeutic agent or diagnostics test component; (3) reverse engineer the product or cause the product to be reverse engineered; or (4) use the product for purposes other than what is indicated in this Limited Use Label License. Life Technologies is not aware of Intellectual Property ("IP") that would be infringed by the manufacture or sale of its products. Customers are urged to perform an IP search and analysis specific to their manufacturing processes and target biologic products. Should that analysis require information about Life Technologies products, Life Technologies will provide to the customer's IP counsel the information necessary to analyze any relevant IP in a manner that protects Life Technologies proprietary information. The purchaser is responsible for obtaining all regulatory approvals necessary for any therapeutic or diagnostic use of the protein (or biological material) manufactured using this product. For information on obtaining additional rights, please contact outlicensing@thermofisher.com, Licensing and Commercial Supply, Thermo Fisher Scientific, 5823 Newton Drive, Carlsbad, CA, 92008, United States.

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Citations & References (1)

Citations & References
Abstract
Adenovirus Fiber Disrupts CAR-Mediated Intercellular Adhesion Allowing Virus Escape.
Authors: Walters Robert; Freimuth Paul; Moninger Thomas; Ganske Ingrid; Zabner Joseph; Welsh Michael;
Journal:Cell
PubMed ID:12297051
Adenovirus binds its receptor (CAR), enters cells, and replicates. It must then escape to the environment to infect a new host. We found that following infection, human airway epithelia first released adenovirus to the basolateral surface. Virus then traveled between epithelial cells to emerge on the apical surface. Adenovirus fiber ... More
1 total citations

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