Ensayo de proliferación celular CyQUANT™ NF
Ensayo de proliferación celular CyQUANT™ NF
Invitrogen™

Ensayo de proliferación celular CyQUANT™ NF

El kit de ensayo de proliferación celular CyQUANT™ NF proporciona un método rápido y sensible para el recuento de célulasMás información
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Número de catálogoTipo de célulaCantidad
C35007NF Cell200 Assays
C35011Direct CellUn kit
C35013Direct Red Cell10 Microplacas
C35012Direct Cell100 Microplates Kit
C7026For cells in culture1000 ensayos
C35006NF Cell1000 Assays
C7027Cell Lysis Buffer50 mL
Número de catálogo C35007
Precio (EUR)
312,00
Each
-
Añadir al carro de la compra
Tipo de célula:
NF Cell
Cantidad:
200 Assays
Recurring order eligible. Learn more »
Precio (EUR)
312,00
Each
Añadir al carro de la compra
Ask our AI about this Product
El kit de ensayo de proliferación celular CyQUANT™ NF proporciona un método rápido y sensible para el recuento de células en una población y la medición de la proliferación en formato de microplaca.

Características del ensayo de proliferación celular CyQUANT™ NF:

• Más sensible que los ensayos MTT o alamarBlue™
• Rango de detección lineal de 100 a 20.000 células por pocillo (microplaca de 96 pocillos)
• Permite completar los ensayos en una hora

Un método rápido y sencillo para medir la proliferación celular
El ensayo de proliferación celular CyQUANT™ NF no requiere lisis celular, incubaciones largas, radioactividad ni eliminación de tinte de las células. El ensayo CyQUANT™ NF elimina el paso de lisis celular con congelación-descongelación del ensayo de proliferación celular CyQUANT™ original utilizando un tinte fijador de ADN y permeable en célula con un reactivo de permeabilización de la membrana de plasma. El ensayo de proliferación celular CyQUANT™ NF se puede utilizar en cualquiera de los formatos de microplacas de 96 pocillos o 384 pocillos, y está disponible en dos configuraciones: un kit de ensayo 200 (C35007) para pequeños tamaños de muestra y un kit de ensayo 1000 (C35006) para aplicaciones de alto rendimiento.

alamarBlue™ es una marca registrada de TREK Diagnostic Systems.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Tipo de célulaNF Cell
Método de detecciónFluorescente
Tipo de coloranteOtras etiquetas o colorantes
Excitación/emisión480/520 nm
FormatoPlaca de 384 pocillos, placa de 96 pocillos
Cantidad200 Assays
Condiciones de envíoTemperatura ambiente
Para utilizar con (aplicación)Ensayo de proliferación
Para utilizar con (equipo)Lector de microplacas, HTS Reader
Línea de productosCyQUANT™
Tipo de productoEnsayo de proliferación celular
Unit SizeEach
Contenido y almacenamiento
Almacenar en el refrigerador (2 °C a 8 °C) y proteger de la luz.
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Certificados

N.º de loteCertificate TypeDateCatalog Number(s)
3192618Certificate of Analysis29 jun 2025C35011
3148807Certificate of Analysis03 may 2025C7026
3154562Certificate of Analysis10 abr 2025C35007
3143926Certificate of Analysis04 abr 2025C35013
3026208Certificate of Analysis18 nov 2024C35011
Se muestran 5 resultados, busque arriba un certificado específico

Hojas de datos de seguridad

Preguntas frecuentes

The original CyQUANT assay provides sensitive detection of cells over a 1000-fold linear dynamic range. In this assay, a freeze-thaw cell lysis step is required to facilitate the interaction of the CyQUANT GR dye with DNA. The CyQUANT NF assay avoids this freeze-thaw step by using a DNA binding dye in combination with a plasma membrane permeabilization reagent. The CyQUANT NF protocol requires only aspiration of growth medium (for adherent cells), replacement with dye binding solution, incubation for 30-60 minutes, and then measurement of fluorescence in a microplate reader. The assay is designed to produce a linear analytical response from at least 100-20,000 cells per well in most cell lines in a 96-well microplate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

PrestoBlue Cell Viability Stain and CyQUANT Cell Proliferation Assay Kit can be used. We also offer a Neurite Outgrowth Staining Kit (Cat. No. A15001). More information about our different assays for neuronal cell health can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/neuroscience/neuronal-cell-health-assays.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (111)

Citations & References
Abstract
KIF14 messenger RNA expression is independently prognostic for outcome in lung cancer.
Authors:Corson TW,Zhu CQ,Lau SK,Shepherd FA,Tsao MS,Gallie BL
Journal:Clinical cancer research : an official journal of the American Association for Cancer Research
PubMed ID:17545527
Growth factor-induced proliferation in corneal epithelial cells is mediated by 12(S)-HETE.
Authors:Ottino P,Taheri F,Bazan HE
Journal:Experimental eye research
PubMed ID:12697425
PURPOSE: Previous studies in our laboratory have shown that 12(S)-hydroxyeicosatetraenoic acid (12(S)-HETE), a product of 12-lipoxygenase (12-LOX) activity, is the predominant metabolite formed in rabbit corneas after injury. The present study was undertaken to investigate the effects of epidermal growth factor (EGF), hepatocyte growth factor (HGF), and keratinocyte growth factor ... More
Caspase activation contributes to delayed death of heat-stressed striatal neurons.
Authors:White MG, Emery M, Nonner D, Barrett JN
Journal:J Neurochem
PubMed ID:14622126
'Hyperthermia can contribute to brain damage both during development and post-natally. We used rat embryonic striatal neurons in culture to study mechanisms underlying hyperthermia-induced neuronal death. Heat stress at 43 degrees C for 2 h produced no obvious signs of damage during the first 12 h after the stress, but ... More
Endothelial cell surface F1-F0 ATP synthase is active in ATP synthesis and is inhibited by angiostatin.
Authors:Moser TL, Kenan DJ, Ashley TA, Roy JA, Goodman MD, Misra UK, Cheek DJ, Pizzo SV
Journal:Proc Natl Acad Sci U S A
PubMed ID:11381144
'Angiostatin blocks tumor angiogenesis in vivo, almost certainly through its demonstrated ability to block endothelial cell migration and proliferation. Although the mechanism of angiostatin action remains unknown, identification of F(1)-F(O) ATP synthase as the major angiostatin-binding site on the endothelial cell surface suggests that ATP metabolism may play a role ... More
Overexpression of Na(+)/K (+)-ATPase parallels the increase in sodium transport and potassium recycling in an in vitro model of proximal tubule cellular ageing.
Authors:Silva E, Gomes P, Soares-da-Silva P,
Journal:J Membr Biol
PubMed ID:17334838
'Na(+)/K(+)-ATPase plays a key role in the transport of Na(+) throughout the nephron, but ageing appears to be accompanied by changes in the regulation and localization of the pump. In the present study, we examined the effect of in vitro cell ageing on the transport of Na(+) and K(+) ions ... More
111 total citations

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