Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Número de catálogo | Tipo de célula | Cantidad |
---|---|---|
C35007 | NF Cell | 200 Assays |
C35011 | Direct Cell | Un kit |
C35013 | Direct Red Cell | 10 Microplacas |
C35012 | Direct Cell | 100 Microplates Kit |
C7026 | For cells in culture | 1000 ensayos |
C35006 | NF Cell | 1000 Assays |
C7027 | Cell Lysis Buffer | 50 mL |
The original CyQUANT assay provides sensitive detection of cells over a 1000-fold linear dynamic range. In this assay, a freeze-thaw cell lysis step is required to facilitate the interaction of the CyQUANT GR dye with DNA. The CyQUANT NF assay avoids this freeze-thaw step by using a DNA binding dye in combination with a plasma membrane permeabilization reagent. The CyQUANT NF protocol requires only aspiration of growth medium (for adherent cells), replacement with dye binding solution, incubation for 30-60 minutes, and then measurement of fluorescence in a microplate reader. The assay is designed to produce a linear analytical response from at least 100-20,000 cells per well in most cell lines in a 96-well microplate.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
PrestoBlue Cell Viability Stain and CyQUANT Cell Proliferation Assay Kit can be used. We also offer a Neurite Outgrowth Staining Kit (Cat. No. A15001). More information about our different assays for neuronal cell health can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/neuroscience/neuronal-cell-health-assays.html).
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
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