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HEPES (1M)
HEPES (1M)
Gibco™

HEPES (1M)

Gibco™ HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. The additionRead more
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Catalog NumberQuantity
15630056
also known as 15630-056
100 mL
Catalog number 15630056
also known as 15630-056
Price (EUR)
104,00
Each
Estimated availability date 01-Aug-2025
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Quantity:
100 mL
Price (EUR)
104,00
Each
Add to cart
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Gibco™ HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. The addition of 10–25 mM HEPES provides extra buffering capacity when cell culture requires extended periods of manipulation outside of a CO2 incubator. HEPES is a good buffering choice for many cell culture systems because it is membrane impermeable, has limited effect on biochemical reactions, is chemically and enzymatically stable, and has very low visible and UV light absorbance.

Dual-site cGMP Manufacturing and Quality System
Gibco™ HEPES is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco™ HEPES product made in our Grand Island facility (15630-080). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Chemical Name or MaterialHEPES Buffer
Shelf Life24 Months
Recommended StorageStorage conditions: 2°C to 8°C
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture
pH6 to 8
Physical FormLiquid
Quantity100 mL
SterilitySterile
Unit SizeEach
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Frequently asked questions (FAQs)

Is HEPES (1 M) in distilled water or in saline solution?

1M HEPES is in water with a pH range of 7.2 - 7.5.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the endotoxin specification for HEPES (1 M) (Cat. Nos. 15630080, 15630106, and 15630130)?

Sorry, we do not test endotoxin levels for this product.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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Certificates

Lot #Certificate TypeDateCatalog Number(s)
3209553Certificate of AnalysisJun 27, 202515630106, 15630080, 15630049, 15630056
3140675Certificate of AnalysisJun 19, 202515630106, 15630080, 15630049, 15630056
3224738Certificate of AnalysisJun 14, 202515630106, 15630080, 15630049, 15630056
3118258Certificate of AnalysisJun 04, 202515630106, 15630080, 15630049, 15630056
3224734Certificate of AnalysisJun 01, 202515630106, 15630080, 15630049, 15630056
5 results displayed, search above for a specific certificate

Safety Data Sheets

Citations & References (2)

Citations & References
Abstract
Preparation and characterization of toxic Abeta aggregates for structural and functional studies in Alzheimer's disease research.
Authors:Jan A, Hartley DM, Lashuel HA,
Journal:Nat Protoc
PubMed ID:20539293
'The amyloid cascade hypothesis, supported by strong evidence from genetics, pathology and studies using animal models, implicates amyloid-beta (Abeta) oligomerization and fibrillogenesis as central causative events in the pathogenesis of Alzheimer''s disease (AD). Today, significant efforts in academia, biotechnology and the pharmaceutical industry are devoted to identifying the mechanisms by ... More
Investigating conversion of mechanical force into biochemical signaling in three-dimensional chondrocyte cultures.
Authors:Bougault C, Paumier A, Aubert-Foucher E, Mallein-Gerin F,
Journal:Nat Protoc
PubMed ID:19478808
The culture of chondrocytes embedded within agarose hydrogels maintains chondrocytic phenotype over extended periods and allows analysis of the chondrocyte response to mechanical forces. The mechanisms involved in the transduction of a mechanical stimulus to a physiological process are not completely deciphered. We present protocols to prepare and characterize constructs ... More
2 total citations

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