Millennium™ RNA Markers

The Ambion® RNA Millennium™ Markers are designed to provide accurate size determination of single-stranded RNA transcripts from 0.5 to 9Read more

Catalog Number	Quantity
AM7150	50 μL

Catalog number AM7150

Price (HKD)

2,027.00

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Quantity:

50 μL

Request bulk or custom format

Price (HKD)

2,027.00

Add to cart

The Ambion® RNA Millennium™ Markers are designed to provide accurate size determination of single-stranded RNA transcripts from 0.5 to 9 kb and can be used in any northern protocol. Sufficient markers are provided for 25 northern gel lanes. They are a mixture of 10 discrete RNA transcripts with easy-to-remember sizes: 0.5, 1, 1.5, 2, 2.5, 3, 4, 5, 6, and 9 kilobases. The markers may be stained with ethidium bromide either during or after electrophoresis. Because of their quantity and even spacing, these markers are ideal for constructing very accurate standard curves for mRNA size determination. They also provide a reference scale for easy verification and comparison of known bands. 1–2 μg (1–2 μg) of RNA Millennium™ Markers will generate 10 distinct bands on a 1% denaturing agarose gel with ethidium bromide staining. All marker lots undergo stringent nuclease testing and are shown to be stable overnight at 37°C.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration1 μg/μl

Gel CompatibilityFormaldehyde-Containing Gels, Denaturing Agarose Gels

Product LineAmbion™, Millennium Markers™

Product TypeRNA Marker

Quantity50 μL

Ready to LoadNo

Shipping ConditionDry Ice

Size Range0.5 to 9 kb

Unit SizeEach

Contents & Storage

Store at below –70°C.

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Frequently asked questions (FAQs)

Why are my RNA bands not sharp?

(1) RNA was not completely denatured. Electrophorese RNA under denaturing conditions. Use urea, formamide or formaldehyde gels, or glyoxal-treated RNA.

(2) For glyoxal-treated RNA, a buffer gradient formed during electrophoresis. Recirculate buffer during electrophoresis to prevent gradient formation.

What is the cause of extra bands when using an RNA ladder?

Extra bands appear in RNA Ladders for a few reasons:

(1) RNA was not completely denatured. Electrophorese RNA under denaturing conditions. Use urea, formamide or formaldehyde gels, or glyoxal-treated RNA.

(2) Extra bands may be a result of using formaldehyde that is not fresh; the pH becomes acidic in older formaldehyde. In our hands, when fresh formaldehyde with neutral pH was used, the extra bands disappeared.

(3) Alternatively, if the extra bands appear after hybridization, it could be that the gel purified probe contains contaminating vector DNA (pUC or pBR) that hybridizes to RNA Ladder template DNA.

Why are some of the RNA marker bands not visible?

Missing RNA bands may be due to:

(1) A small amount of RNA diffused out of gel during extended destaining. Minimize destaining time. Destaining for 2 hours is sufficient for most applications.

(2) RNA bands of similar molecular size were not resolved. Use the correct gel type and denaturing conditions.

Why are the RNA bands disappearing when looking and photographing a gel on a UV box?

RNA was exposed to UV light for extended periods of time. Minimize exposure to UV light. Stain and destain gels in the dark and photograph the gel immediately.

Why are the RNA marker bands so faint?

Many factors could affect the intensity of the bands as summarized below.

(1) Insufficient RNA was loaded on the gel. Increase the amount of RNA loaded.

(2) RNA was degraded. Avoid nuclease contamination of the RNA standards. Store RNA at -70 degrees C in formamide. Deionize formamide and glyoxal, store aliquots at -20 degrees C.

(3) RNA was electrophoresed off the gel. Electrophorese the gel for less time, at a lower voltage, or in a higher percentage gel.

(4) For ethidium bromide-stained RNA, improper UV light source was used. Use short-wavelength (254 nm) UV light. For ethidium bromide-stained RNA, improper staining and destaining conditions were used. Stain in the dark with 5 mg/mL ethidium bromide. For thin (3.2 mm) formaldehyde gels, stain 5 min and destain 1 h. For thicker gels, stain 30 min and destain 2 hr. To reduce background staining, use 0.66 M formaldehyde instead of 2.2 M formaldehyde in gels. For glyoxal RNA gels, stain and destain in 0.5 M ammonium acetate to help reduce background staining.

(5) For radiolabeled RNA, an improper labeling method was used.

View all Millennium™ RNA Markers FAQs