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証明書
SDS
Invitrogen™
iBlot™ Transfer Stack, PVDF, regular size
The iBlot™ Transfer Stacks are used to transfer proteins using the iBlot™ Gel Transfer Device. The iBlot™ Transfer Stacks are詳細を見る
| 製品番号（カタログ番号） | 数量 |
|---|---|
| IB401001 | 10 sets |
製品番号（カタログ番号） IB401001
価格（JPY）お問い合わせください ›
39,300
Each
数量:
10 sets
The iBlot™ Transfer Stacks are used to transfer proteins using the iBlot™ Gel Transfer Device. The iBlot™ Transfer Stacks are disposable stacks that come in three varieties for different applications: iBlot™ Gel Transfer Stacks have integrated PVDF or nitrocellulose transfer membranes to perform dry blotting of proteins, iBlot™ DNA Transfer Stacks have an integrated nylon transfer membrane to perform dry blotting of DNA, and iBlot™ Western Detection Stacks are used to perform Western detection on previously blotted nitrocellulose or PVDF membranes. Each iBlot™ Transfer Stack contains a copper electrode and appropriate cathode and anode buffers in the gel matrix to allow fast, reliable transfer of proteins or DNA.
PVDF membrane
The PVDF membrane (0.2 μm pore size, low fluorescence) in this stack has higher binding capacity than nitrocellulose. The PVDF membrane is preactivated and ready for use without any pretreatment with alcohols. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, or radiolabeling. The proteins bind to the membrane due to hydrophobic interactions. The protein binding capacity is 240 μg/cm2.
Transfer stack sizes
The regular size (13 cm x 8.3 cm) is used for all Novex™ midi-gel types (or equivalent) and E-PAGE™ 48 or 96 Gels. The mini size (8 cm x 8 cm) is used for all Novex™ mini-gels (or equivalent).
PVDF membrane
The PVDF membrane (0.2 μm pore size, low fluorescence) in this stack has higher binding capacity than nitrocellulose. The PVDF membrane is preactivated and ready for use without any pretreatment with alcohols. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, or radiolabeling. The proteins bind to the membrane due to hydrophobic interactions. The protein binding capacity is 240 μg/cm2.
Transfer stack sizes
The regular size (13 cm x 8.3 cm) is used for all Novex™ midi-gel types (or equivalent) and E-PAGE™ 48 or 96 Gels. The mini size (8 cm x 8 cm) is used for all Novex™ mini-gels (or equivalent).
研究用途にのみご使用ください。診断目的には使用できません。
仕様
グリーン機能持続可能な廃棄
数量10 sets
出荷条件室温
Dimensions (LxW)標準サイズ、13.5 x 8 cm
フォーマット転写スタック
長さ（メートル法）13.5 cm
材料PVDF膜
孔径0.2 μm
製品ラインiBlot™
対応可能10 Blots
幅（メートル法）8 cm
Unit SizeEach
組成および保存条件
転写スタック10セット入り1箱。室温保存。
Introducing iBlot 3 Western Blot Transfer System
Featuring higher throughput and built-in cooling for consistent protein transfer
Learn more ›
Limited Time Savings!
Enjoy savings on the remaining stock of iBlot 2 devices, available while supplies last.
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図
Transfers using the iBlot® system
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ドキュメントおよびダウンロード
証明書
Search by lot number or partial lot number
ロット番号Certificate TypeDateCatalog Number(s)
PR080625-01Certificate of Analysis2025年6月09日IB401001
PR150525-01Certificate of Analysis2025年5月19日IB401001
PR240425-01Certificate of Analysis2025年4月27日IB401001
PR060325-01Certificate of Analysis2025年3月11日IB401001
PR041224-01Certificate of Analysis2025年1月31日IB401001
5件の結果が表示されました。 上記から特定の証明書を検索します
Safety Data Sheets
SDSProduct Information
よくあるご質問（FAQ）
Yes, all original iBlot stacks are still available for purchase. You can find them in the original iBlot Gel Transfer Device Reagents and Resources (http://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/transfer-proteins-western-blot/iblot-dry-blotting-system/reagents-resources-original-iblot-gel-transfer-device.html). However, please note that they are not compatible with the iBlot 2 Gel transfer Device.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
No. Do not use iBlot Transfer Stacks in the iBlot 2 Gel Transfer Device, and do not mix components between iBlot Transfer Stacks and iBlot 2 Transfer Stacks.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Proteins larger than ~150 kDa migrate more slowly than smaller proteins. Therefore, more time is required to transfer them from gel to membrane. We recommend extending the transfer time by 8 to 10 minutes for optimal transfer of proteins >150 kDa using the iBlot Dry Blotting System.
To enhance transfer efficiency, we also recommend adding an equilibration (gel-soaking) step between electrophoresis and western transfer and using NuPAGE Invitrogen 3 - 8% Tris-acetate gels for electrophoresis. We have an application note available, titled “Transferring Large and Small Proteins Using the iBlot Dry Blotting System”. To download the PDF, see the iBlot Reagents Resources page. (http://www.thermofisher.com/us/en/home/life-science/protein-expression-and-analysis/western-blotting/western-blot-transfer/iblot-dry-blotting-system/original-iblot-resources.html)
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
This may be a result of insufficient blocking or nonspecific binding. We suggest trying our WesternBreeze Blocker/Diluent (Cat. No. WB7050). We have been using it with good success. Additionally, you should optimize primary and secondary antibody concentrations as generally recommended for any new blotting technique. Many cases of high background can be resolved by further diluting one or both antibody preparations.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
In theory, you can replace the membrane provided in your iBlot Transfer Stack with any membrane that is compatible with western blotting. To do this, cut the alternative membrane to match the size of your gel, and wet the membrane. Then, either place the alternative membrane on top of the integrated membrane, or carefully remove the integrated membrane from the gel matrix with forceps and replace it with the new membrane. Note that we only support the use of iBlot Transfer Stacks when they are used with the provided instructions.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.