Power SYBR™ Green PCR Master Mix

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Applied Biosystems™

Power SYBR™ Green PCR Master Mix

Get superior nucleic acid quantitation with Applied Biosystems™ Power SYBR™ Green Master Mix. Power SYBR™ Master Mix offers superior sensitivityRead more

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Catalog Number	Quantity
4367659	1 x 5 mL
4368577	1 x 1 mL
4368706	2 x 5 mL
4368702	5 x 5 mL
4368708	10 x 5 mL
4367660	1 x 50 mL

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Catalog number 4367659

Price (KRW)

503,000

Each

In stock

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Quantity:

1 x 5 mL

Price (KRW)

503,000

Each

Add to cart

Get superior nucleic acid quantitation with Applied Biosystems™ Power SYBR™ Green Master Mix. Power SYBR™ Master Mix offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations.

• Everything you need for SYBR™ Green dye–based PCR amplification and detection in a convenient single-tube format. Power SYBR™ Green PCR Master Mix contains all of the components, excluding the template and primers, for superior SYBR™ Green reagent-based real-time PCR
• The optimized formulation contains highly purified AmpliTaq Gold™ DNA Polymerase, LD to offer greater sensitivity than classic SYBR™ Green PCR master mix
• Power SYBR™ Green PCR Master Mix contains a blend of dTTP/dUTP that is compatible with AmpErase™ UNG, to minimize carryover contamination
• Includes a proprietary version of ROX™ dye, an internal passive reference, to normalize non-PCR–related fluorescence fluctuations to minimize well-to-well variability that result from a variety of causes, such as pipetting error and sample evaporation
• Power SYBR™ Green reagent-based PCR chemistry easily replaces the existing SYBR™ Green PCR Master Mix in Applied Biosystems™ protocols using the same setup and thermal cycling conditions

Alternative product
Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Power SYBR Green PCR Master Mix and added additional capabilities for your gene expression analysis.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment)7300 System, 7500 System, 7700 System, 7900HT Fast System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System

FormatTube

No. of Reactions200 Reactions

Passive Reference DyeROX (Pre-mixed)

PolymeraseAmpliTaq Gold DNA Polymerase, AmpliTaq Gold LD

Product LineSYBR™

Product TypeReal Time PCR SYBR Master Mix

Purity or Quality GradeLD (Low DNA)

Quantity1 x 5 mL

Sample TypeDNA (Genomic), cDNA

Shipping ConditionWet Ice

Sufficient For200 Reactions of 50 μL Each

Volume1 x 5 mL

Concentration2X

Detection MethodSYBR

For Use With (Application)Gene Expression

GC-Rich PCR PerformanceHigh

Label or DyeSYBR Green

PCR MethodqPCR

Reaction SpeedStandard

Unit SizeEach

Contents & Storage

The 2X mix contains SYBR™ Green 1 Dye, AmpliTaq Gold DNA Polymerase LD, dNTPs with dUTP/dTTP blend, Passive Reference 1, and optimized buffer components. Contains 1 × 5 mL, sufficient for 200 reactions of 50 μl each.

Store at -20°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

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Frequently asked questions (FAQs)

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.

View all Power SYBR™ Green PCR Master Mix FAQs