DMEM, powder, high glucose
DMEM, powder, high glucose
Gibco™

DMEM, powder, high glucose

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.자세히 알아보기
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카탈로그 번호수량
1210006110 L
1210004610 x 1 L
카탈로그 번호 12100061
제품 가격(KRW)
54,000
Online offer
Ends: 30-Sep-2025
56,000
할인액 2,000 (4%)
Each
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수량:
10 L
Recurring order eligible. Learn more »
Customize this product
제품 가격(KRW)
54,000
Online offer
Ends: 30-Sep-2025
56,000
할인액 2,000 (4%)
Each
카트에 추가하기
Ask our AI about this Product
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:
WithWithout
• High Glucose• Sodium Pyruvate
• L-glutamine• HEPES
• Phenol Red• Sodium Bicarbonate

The complete formulation is available.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Gibco™ cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

cGMP manufacturing and quality system
DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable DMEM product made in our Scotland facility (52100-021). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

This product is not used for in vitro diagnostic purpose in some countries.
In vitro diagnostic
사양
세포주HeLa, 293, Cos-7, and PC-12
세포 유형Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
Glucose 농도4500 mg/L
제조 품질cGMP-compliant under the ISO 13485 standard
제품라인Gibco™
제품 유형DMEM (Dulbecco's Modified Eagle Medium)
수량10 L
유통 기한36 Months From Date of Manufacture
배송 조건Room Temperature
분류Animal Origin-free
형태Powder
Serum LevelStandard Serum Supplementation
멸균Sterile-filtered
첨가제 포함High Glucose, Glutamine, Phenol Red
첨가제 없음No HEPES, No Sodium Pyruvate, No Sodium Bicarbonate
Unit SizeEach
구성 및 보관
Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
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문서 및 다운로드

Certificates | 증명서

Lot 번호Certificate TypeDateCatalog Number(s)
3166173Certificate of Analysis2025년 6월 28일12100061, 12100046
3156299Certificate of Analysis2025년 6월 05일12100061, 12100046
3123461Certificate of Analysis2025년 5월 14일12100061, 12100046
3123462Certificate of Analysis2025년 5월 11일12100061, 12100046
3066199Certificate of Analysis2025년 4월 09일12100061, 12100046
시험 성적서 관련 5 개의 결과가 검색되었습니다.

Safety Data Sheets | 물질 안전 보건 자료

자주 묻는 질문(FAQ)

Manganese is not present in the formulation of our catalog DMEM media products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Expiration date of most Gibco reconstituted dry format media (AGT or DPM) has not been established; end users should assess performance and stability of this reconstituted media in a system that is relevant to their process. A dry format product (either DPM or AGT) will age upon storage and while we may not be able to detect which component(s) degrade since we can't measure every component, that doesn't mean that the reconstituted liquid will have the same stability as a fresh liquid made by direct weigh methods.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

인용 및 참조 문헌 (10)

인용 및 참조 문헌
Abstract
Galectin-3 Translocates to the Perinuclear Membranes and Inhibits Cytochrome c Release from the Mitochondria. A ROLE FOR SYNEXIN IN GALECTIN-3 TRANSLOCATION.
Authors: Yu Fei; Finley Russell L Jr; Raz Avraham; Kim Hyeong-Reh Choi;
Journal:J Biol Chem
PubMed ID:11839755
'Galectin-3 is a multifunctional oncogenic protein found in the nucleus and cytoplasm and also the extracellular milieu. Although recent studies demonstrated an anti-apoptotic activity of galectin-3, neither the functional site nor the mechanism of how galectin-3 regulates apoptosis is known. In this study, we examined the subcellular localization of galectin-3 ... More
Functions of Transforming Growth Factor-beta Family Type I Receptors and Smad Proteins in the Hypertrophic Maturation and Osteoblastic Differentiation of Chondrocytes.
Authors: Valcourt Ulrich; Gouttenoire Jerome; Moustakas Aristidis; Herbage Daniel; Mallein-Gerin Frederic;
Journal:J Biol Chem
PubMed ID:12082094
'We investigated the effects of bone morphogenetic protein (BMP)-2, a member of the transforming growth factor-beta superfamily, on the regulation of the chondrocyte phenotype, and we identified signaling molecules involved in this regulation. BMP-2 triggers three concomitant responses in mouse primary chondrocytes and chondrocytic MC615 cells. First, BMP-2 stimulates expression ... More
Cloning and characterization of freac-9 (FKHL17), a novel kidney-expressed human forkhead gene that maps to chromosome 1p32-p34.
Authors: Ernstsson S; Betz R; Lagercrantz S; Larsson C; Ericksson S; Cederberg A; Carlsson P; Enerbäck S;
Journal:Genomics
PubMed ID:9403061
'We describe the cloning of a near full-length cDNA of 4258 nucleotides encoding freac-9 (HGMW-approved symbol FKHL17), a novel human forkhead gene. The 5'' untranslated region is unusual since it is very long, 2127 nucleotides, and contains 15 upstream AUG codons. Hybridization to a panel consisting of RNA derived from ... More
Vasohibin 2 is transcriptionally activated and promotes angiogenesis in hepatocellular carcinoma.
Authors:Xue X, Gao W, Sun B, Xu Y, Han B, Wang F, Zhang Y, Sun J, Wei J, Lu Z, Zhu Y, Sato Y, Sekido Y, Miao Y, Kondo Y
Journal:Oncogene
PubMed ID:22614011
'Hepatocellular carcinoma (HCC) typically relies on angiogenesis for its malignant behavior, including growth and metastasis. Vasohibin 2 (VASH2) was previously identified as an angiogenic factor, but its role in tumorigenesis is unknown. Using quantitative PCR and western blot analyses, we found that VASH2 is overexpressed in HCC cells and tissues. ... More
Lentiviral vector production, titration, and transduction of primary neurons.
Authors:Ding B, Kilpatrick DL
Journal:
PubMed ID:23681623
'Lentiviral vectors have become very useful tools for transgene delivery. Based on their ability to transduce both dividing and nondividing cells and to produce long-term transgene expression, lentiviruses have found numerous applications in the biomedical sciences, including developmental neuroscience. This protocol describes how to prepare lentiviral vectors by calcium phosphate ... More
10 total citations

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