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DSS (disuccinimidyl suberate), No-Weigh™ Format
DSS (disuccinimidyl suberate), No-Weigh™ Format
Thermo Scientific™

DSS (disuccinimidyl suberate), No-Weigh™ Format

Thermo Scientific Pierce DSS, also called disuccinimidyl suberate, is noncleavable and membrane permeable crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS)자세히 알아보기
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카탈로그 번호수량
A3926710 x 2 mg
2165550 mg
215551 g
카탈로그 번호 A39267
제품 가격(KRW)
132,000
Online offer
Ends: 30-Sep-2025
155,000
할인액 23,000 (15%)
Each
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수량:
10 x 2 mg
Recurring order eligible. Learn more »
제품 가격(KRW)
132,000
Online offer
Ends: 30-Sep-2025
155,000
할인액 23,000 (15%)
Each
카트에 추가하기
Ask our AI about this Product
Thermo Scientific Pierce DSS, also called disuccinimidyl suberate, is noncleavable and membrane permeable crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester at each end of an 8-carbon spacer arm.

NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxysuccinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS-ester crosslinking reagents. DSS is first dissolved in an organic solvent such as DMF or DMSO, then added to the aqueous crosslinking reaction. BS3, the water soluble analog of DSS is also available for applications that require a hydrophilic crosslinker (e.g. to effect cell-surface crosslinking). DSS and BS3 have essentially identical crosslinking activity toward primary amines.

Features of disuccinimidyl suberate:

Reactive groups: NHS ester (both ends)
Reactive towards: amino groups (primary amines)
• Amine reactive sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
• Membrane-permeable, allowing for intracellular crosslinking
• High purity, crystalline reagent can be used to create high-purity conjugates
• Non-cleavable
• Water-insoluble (dissolve first in DMF or DMSO); compare to BS3 (Sulfo-DSS)

Applications:
• Chemical crosslinking of intracellular proteins prior to cell lysis and immunoprecipitation
• 'Fix' protein interactions to allow identification of weak or transient protein interactions
• Protein crosslinking to create bioconjugates via single-step reactions
• Immobilize proteins onto amine-coated surfaces

Properties of DSS
• Alternative names: Disuccinimidyl suberate
• Molecular formula: C16H20N2O8
• Molecular weight: 368.35
• Spacer arm length: Å (8 atoms)
• CAS number: 68528-80-3
• Reactive groups: NHS esters, react with primary amines at pH 7.0–9.0

DSS Specifications
We manufacture DSS to the highest specifications to produce the most specific bioconjugates, ensure the integrity of your data and to provide you with the highest degree of consistency. Each lot of DSS crosslinker is tested to meet the following minimum specifications:
Identity: IR scan shows only peaks characteristic of the structure and functional groups of DSS
Purity: > 90% by quantitative NMR (the highest standard in crosslinking purity)
Solubility: > 9.2 mg/mL in DMF and DMSO, clear and colorless solution

Product References
Crosslinker Application Guide -- search for recent literature references for this product

Related Products
BS3 (bis(sulfosuccinimidyl)suberate)
For Research Use Only. Not for use in diagnostic procedures.
사양
세포 투과성Yes
설명DSS
형태Solid
라벨링 방법Chemical Labeling
분자량368.35
페길레이티드No
제품라인No-Weigh™
수량10 x 2 mg
반응성 부분NHS Ester
배송 조건Ambient
용해도DMF, DMSO
스페이서 암 길이11.4 Å
수용성No
화학물질 반응성Amine-Amine
CleavableNo
교차결합제 유형Homobifunctional
형식Standard, Single-use
제품 유형Crosslinker
스페이서Medium (10 to 30 Å)
Unit SizeEach
구성 및 보관
Upon receipt store desiccated at 4°C.
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자주 묻는 질문(FAQ)

What is the protocol for crosslinking IgG to Dynabeads from the Dynabeads Protein G Immunoprecipitation Kit (Cat. No. 10007D) using DSS (disuccinimidyl suberate)?

For crosslinking the antibody to Protein G Dynabeads, please refer to the crosslinking protocol with BS3 (bis(sulfosuccinimidyl)suberate) or Sulpha-DSS, which is an analogue of DSS. The only difference between these two molecules is that DSS is water insoluble and needs to be solubilized in DMSO before use. You can find the protocol at the following link.

Find additional tips, troubleshooting help, and resources within our Protein Immunoprecipitation (IP), Co-Immunoprecipitation (Co-IP), and Pulldown Support Center

Can you provide the shelf-life for DSS (disuccinimidyl suberate)?

DSS (disuccinimidyl suberate) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How does a product with "No-Weigh" format reduce my concern over reagent stability?

The No-Weigh product format, where small amounts of reagent are pre-weighed and packaged separately, prevents the contamination and loss of reagent reactivity over time associated with repetitive opening and closing of a single vial. This format enables use of a fresh vial of reagent each time and eliminates the hassle of weighing out small amounts of reagent. Please view additional information and the video at: https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-labeling-crosslinking/no-weigh-packaging.html

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

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문서 및 다운로드

Certificates | 증명서

Lot 번호Certificate TypeDateCatalog Number(s)
3214364Certificate of Analysis2025년 7월 22일A39267
3213219Certificate of Analysis2025년 7월 08일21655
3214235Certificate of Analysis2025년 6월 26일21555
3212151Certificate of Analysis2025년 6월 10일A39267
AB406211Certificate of Analysis2025년 3월 12일A39267
시험 성적서 관련 5 개의 결과가 검색되었습니다.

Safety Data Sheets | 물질 안전 보건 자료

인용 및 참조 문헌 (7)

인용 및 참조 문헌
Abstract
Anti-human activin receptor-like kinase 1 (ALK1) antibody attenuates bone morphogenetic protein 9 (BMP9)-induced ALK1 signaling and interferes with endothelial cell sprouting.
Authors:van Meeteren LA, Thorikay M, Bergqvist S, Pardali E, Stampino CG, Hu-Lowe D, Goumans MJ, ten Dijke P
Journal:J Biol Chem
PubMed ID:22493445
'Genetic and molecular studies suggest that activin receptor-like kinase 1 (ALK1), a transforming growth factor ß (TGF-ß) type I receptor, and endoglin, a TGF-ß co-receptor, play an essential role in vascular development and pathological angiogenesis. Several agents that interfere with ALK1 and endoglin function are currently in clinical trials for ... More
The MUC1-C oncoprotein binds to the BH3 domain of the pro-apoptotic BAX protein and blocks BAX function.
Authors:Ahmad R, Alam M, Rajabi H, Kufe D
Journal:J Biol Chem
PubMed ID:22544745
'The pro-apoptotic BAX protein contains a BH3 domain that is necessary for its dimerization and for activation of the intrinsic apoptotic pathway. The MUC1 (mucin 1) heterodimeric protein is overexpressed in diverse human carcinomas and blocks apoptosis in the response to stress. In this study, we demonstrate that the oncogenic ... More
Crossveinless 2 regulates bone morphogenetic protein 9 in human and mouse vascular endothelium.
Authors:Yao Y, Jumabay M, Ly A, Radparvar M, Wang AH, Abdmaulen R, Boström KI,
Journal:Blood
PubMed ID:22474252
The importance of morphogenetic proteins (BMPs) and their antagonists in vascular development is increasingly being recognized. BMP-4 is essential for angiogenesis and is antagonized by matrix Gla protein (MGP) and crossveinless 2 (CV2), both induced by the activin receptor like-kinase 1 (ALK1) when stimulated by BMP-9. In this study, however, ... More
Crosslinking-MS analysis reveals RNA polymerase I domain architecture and basis of rRNA cleavage.
Authors:Jennebach S, Herzog F, Aebersold R, Cramer P
Journal:Nucleic Acids Res
PubMed ID:22396529
RNA polymerase (Pol) I contains a 10-subunit catalytic core that is related to the core of Pol II and includes subunit A12.2. In addition, Pol I contains the heterodimeric subcomplexes A14/43 and A49/34.5, which are related to the Pol II subcomplex Rpb4/7 and the Pol II initiation factor TFIIF, respectively. ... More
Binding of calmodulin to the D2-dopamine receptor reduces receptor signaling by arresting the G protein activation switch.
Authors:Bofill-Cardona E, Kudlacek O, Yang Q, Ahorn H, Freissmuth M, Nanoff C
Journal:J Biol Chem
PubMed ID:10926927
Signaling by D(2)-dopamine receptors in neurons likely proceeds in the presence of Ca(2+) oscillations. We describe here the biochemical basis for a cross-talk between intracellular Ca(2+) and the D(2) receptor. By activation of calmodulin (CaM), Ca(2+) directly inhibits the D(2) receptor; this conclusion is based on the following observations: (i) ... More
7 total citations

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