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DreamTaq PCR Master Mixes (2X)
Thermo Scientific DreamTaq PCR Master Mix (2X) is a ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq buffer, MgCl2, and dNTPs.
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| Catalog Number | Color | Quantity |
|---|---|---|
| K1072 | Colorless | 20 x 1.25 mL |
| K1071 | Colorless | 4 x 1.25 mL |
| K1081 | Green | 4 x 1.25 mL |
| K1082 | Green | 20 x 1.25 mL |
Catalog number K1072
Price (TWD)
18,760.00
Online offer
Ends: 30-Jun-2025
27,500.00Save 8,740.00 (32%)
Each
-
Color:
Colorless
Quantity:
20 x 1.25 mL
Price (TWD)
18,760.00
Online offer
Ends: 30-Jun-2025
27,500.00Save 8,740.00 (32%)
Each
Thermo Scientific DreamTaq PCR Master Mix (2X), available in colorless and green formats, is a ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq buffer, MgCl2, and dNTPs. This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR setup.
The master mix retains all features of DreamTaq DNA Polymerase and is capable of robust amplification up to 6 kb from genomic DNA and up to 20 kb from viral DNA. It ensures higher sensitivity, longer PCR products, and higher yields compared to conventional Taq DNA polymerase. Extensive optimization of reaction conditions is not required.
DreamTaq Green PCR Master Mix (2X) additionally includes 2X DreamTaq Green Buffer with a density reagent and two dyes for monitoring electrophoresis progress.
Features
- Robust amplification with minimal optimization
- High yields of PCR products
- Higher sensitivity compared to conventional Taq DNA polymerase
- Amplification of targets up to 6 kb from genomic DNA and up to 20 kb from viral DNA
- Incorporates modified nucleotides, but does not incorporate dUTP
- Direct loading of PCR product on gel with green format
Applications
- Routine PCR amplification of DNA fragments up to 6 kb
- High throughput PCR
- Genotyping
For Research Use Only. Not for use in diagnostic procedures.
In certain countries use of this product is covered by patents. Purchase of product in these countries includes non-transferable, limited license for using only this amount of product for the purchaser's own internal research.
This product is licensed under one or more U.S. Patents Nos. 5,500,363 and 5,352,778 or corresponding foreign patents. This product is licensed under one U.S. Patent No. 5,436,149 or corresponding foreign patents owned by Takara Bio, Inc.
Patent pending.
Specifications
ColorColorless
Concentration5 U/μL
Fidelity (vs. Taq)1X
Hot StartNo
Overhang3'-A
PolymeraseDreamTaq DNA Polymerase
Quantity20 x 1.25 mL
Reaction FormatSuperMix or Master Mix
Shipping ConditionDry Ice
For Use With (Application)Standard PCR
GC-Rich PCR PerformanceLow
Reaction SpeedStandard
Unit SizeEach
Contents & Storage
• DreamTaq PCR Master Mix (2X), 20 x 1.25 mL
• Nuclease-free water, 20 x 1.25 mL
Store at -20°C.
• Nuclease-free water, 20 x 1.25 mL
Store at -20°C.
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Figures
Comparison of PCR sensitivity and yield with DreamTaq DNA Polymerase and other commercial polymerases
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Certificates
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Lot #Certificate TypeDateCatalog Number(s)
3269448Certificate of AnalysisJun 26, 2025K1082
3260868Certificate of AnalysisJun 13, 2025K1081
3258948Certificate of AnalysisJun 11, 2025K1071
3249885Certificate of AnalysisJun 02, 2025K1082
3248547Certificate of AnalysisMay 29, 2025K1081
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Safety Data Sheets
SDSFrequently asked questions (FAQs)
The error rate of DreamTaq DNA Polymerase is the same as that of Taq DNA Polymerase: 2.2x10^-5 errors per nt per cycle.
Dream Taq PCR Master Mix and Taq PCR Master Mix can be used for direct PCR from bacterial colonies. Simply scrape a tiny amount of a single colony and transfer to a PCR-tube, prefilled with the master mix. Always use at least 3 min at 95 degrees C for initial denaturation.