DMEM, high glucose, pyruvate, no glutamine
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DMEM, high glucose, pyruvate, no glutamine
Gibco™

DMEM, high glucose, pyruvate, no glutamine

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.Read more
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Catalog NumberQuantity
10313039Promo Image10 x 500 mL
10313021Promo Image500 mL
Catalog number 10313039
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312.65
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DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.


This DMEM is modified as follows:
WithWithout
• High Glucose• L-glutamine
• Phenol Red• HEPES
• Sodium Pyruvate 


The complete formulation is available.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell LineHeLa, 293, Cos-7, and PC-12
Cell TypePrimary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Product TypeDMEM (Dulbecco's Modified Eagle Medium)
Quantity10 x 500 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormLiquid
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesHigh Glucose, Phenol Red, Sodium Pyruvate
Without AdditivesNo Glutamine, No HEPES
Unit SizeEach
Contents & Storage
Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
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Certificates

Lot #Certificate TypeDateCatalog Number(s)
3148628Certificate of AnalysisJun 05, 202510313021
3140459Certificate of AnalysisApr 02, 202510313021
3106272Certificate of AnalysisFeb 20, 202510313021
3023664Certificate of AnalysisFeb 19, 202510313021
2537060Certificate of AnalysisJan 29, 202510313021
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Safety Data Sheets

Frequently asked questions (FAQs)

Manganese is not present in the formulation of our catalog DMEM media products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations & References (13)

Citations & References
Abstract
Subcellular targeting of RGS9-2 is controlled by multiple molecular determinants on its membrane anchor, R7BP.
Authors:Song JH, Waataja JJ, Martemyanov KA,
Journal:J Biol Chem
PubMed ID:16574655
'RGS9-2, a member of the R7 RGS protein family of neuronal RGS (Regulators of G protein Signaling), is a critical regulator of G protein signaling. In striatal neurons, RGS9-2 is tightly associated with a novel palmitoylated protein - R7BP (R7 family Binding Protein). Here we report that R7BP acts to ... More
Cloning the vaccinia virus genome as a bacterial artificial chromosome in Escherichia coli and recovery of infectious virus in mammalian cells.
Authors: Domi Arban; Moss Bernard;
Journal:Proc Natl Acad Sci U S A
PubMed ID:12196634
'The ability to manipulate the vaccinia virus (VAC) genome, as a plasmid in bacteria, would greatly facilitate genetic studies and provide a powerful alternative method of making recombinant viruses. VAC, like other poxviruses, has a linear, double-stranded DNA genome with covalently closed hairpin ends that are resolved from transient head-to-head ... More
The regulated secretion and vectorial targeting of neurotrophins in neuroendocrine and epithelial cells.
Authors: Heymach J V Jr; Krüttgen A; Suter U; Shooter E M;
Journal:J Biol Chem
PubMed ID:8810312
'The varied roles that neurotrophins play in the development and activity-dependent plasticity of the nervous system presumably require that the sites and quantity of neurotrophin release be precisely regulated. As a step toward understanding how different neurotrophins are sorted and secreted by neurons, we expressed nerve growth factor (NGF), brain-derived ... More
Tyrphostins protect neuronal cells from oxidative stress.
Authors:Sagara Y, Ishige K, Tsai C, Maher P,
Journal:J Biol Chem
PubMed ID:12121989
'Tyrphostins are a family of tyrosine kinase inhibitors originally synthesized as potential anticarcinogenic compounds. Because tyrphostins have chemical structures similar to those of the phenolic antioxidants, we decided to test the protective efficacy of tyrphostins against oxidative stress-induced nerve cell death (oxytosis). Many commercially available tyrphostins, at concentrations ranging from ... More
The Plexin-B1/Rac interaction inhibits PAK activation and enhances Sema4D ligand binding.
Authors: Vikis Haris G; Li Weiquan; Guan Kun-Liang;
Journal:Genes Dev
PubMed ID:11937491
'The small GTPase Rac has been implicated in growth cone guidance mediated by semaphorins and their receptors. Here we demonstrate that plexin-B1, a receptor for Semaphorin4D (Sema4D), and p21-activated kinase (PAK) can compete for the interaction with active Rac and plexin-B1 can inhibit Rac-induced PAK activation. We have also demonstrated ... More
13 total citations

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