Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Catalog Number | Quantity |
---|---|
11965084 | 1000 mL |
11965092 | 500 mL |
11965118 | 10 x 500 mL |
11965126 | 6 x 1000 mL |
11965167 | 5 L |
11965175 | 10 L |
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM is modified as follows:
With: High Glucose, L-glutamine, Phenol Red
Without: Sodium Pyruvate, HEPES
The complete formulation is available.
Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
We do provide osmolality information on the certificate of analysis. All lots of DMEM, high glucose (Cat. No. 11965xxx) will meet the osmolality specification of 320-355 mOsm/kg.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Manganese is not present in the formulation of our catalog DMEM media products.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
We do not provide stability data for the product once it is opened as it would depend on the usage and storage conditions.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Yes, the medium should be stored in the dark because there are some components in the medium such as HEPES, Tryptophan, and Riboflavin, etc. that are sensitive to light.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.
Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.
Share catalog number, name or link