1x1 image pixel for data collection
DMEM, high glucose
Save up to 29% on your order of cell culture products. View promotion details >
DMEM, high glucose
Gibco™

DMEM, high glucose

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.
Promo ImagePromotion
Promo code:RCELL25
Save up to 29% across the cell culture workflow - research begins with cells
From now until Dec 31, get up to 29% off media, sera, buffers, reagents and other cell culture products to advance your researchLearn More
Have Questions?
Change viewbuttonViewtableView
Catalog NumberQuantity
11965084Promo Image1000 mL
11965092Promo Image500 mL
11965118Promo Image10 x 500 mL
11965126Promo Image6 x 1000 mL
11965167Promo Image5 L
11965175Promo Image10 L
Catalog number 11965084
Price (USD)
55.65
Online Exclusive
60.75
Save 5.10 (8%)
Each
In stock
Add to cart
Quantity:
1000 mL
Recurring order eligible. Learn more »
Customize this product
Price (USD)
55.65
Online Exclusive
60.75
Save 5.10 (8%)
Each
Add to cart
Ask our AI about this Product

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:

With: High Glucose, L-glutamine, Phenol Red

Without: Sodium Pyruvate, HEPES

The complete formulation is available.

Using DMEM

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell LineHeLa, 293, Cos-7, and PC-12
Cell TypePrimary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
Concentration1 X
For Use With (Application)Mammalian Cell Culture
Glucose Concentration4500 mg/L
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Product TypeDMEM (Dulbecco's Modified Eagle Medium)
Quantity1000 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormLiquid
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesHigh Glucose, Glutamine, Phenol Red
Without AdditivesNo HEPES, No Sodium Pyruvate
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Media Formulations
Have questions about this product? Ask our AI assisted search.
Generating response
This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our Privacy Notice.

Customers who viewed this item also viewed



Documents & Downloads

Certificates

Lot #Certificate TypeDateCatalog Number(s)
3140610Certificate of AnalysisJun 07, 202511965092, 11965084, 11965175, 11965167
3157156Certificate of AnalysisJun 04, 202511965092, 11965084, 11965175, 11965167
3013600Certificate of AnalysisMay 30, 202511965092, 11965084, 11965175, 11965167
3144500Certificate of AnalysisMay 29, 202511965092, 11965084, 11965175, 11965167
3142728Certificate of AnalysisMay 07, 202511965092, 11965084, 11965175, 11965167
5 results displayed, search above for a specific certificate

Safety Data Sheets

Frequently asked questions (FAQs)

We do provide osmolality information on the certificate of analysis. All lots of DMEM, high glucose (Cat. No. 11965xxx) will meet the osmolality specification of 320-355 mOsm/kg.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Manganese is not present in the formulation of our catalog DMEM media products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

We do not provide stability data for the product once it is opened as it would depend on the usage and storage conditions.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Yes, the medium should be stored in the dark because there are some components in the medium such as HEPES, Tryptophan, and Riboflavin, etc. that are sensitive to light.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

Citations & References (132)

Citations & References
Abstract
Identification of a novel redox-sensitive gene, Id3, which mediates angiotensin II-induced cell growth.
Authors:Mueller Cornelius; Baudler Stephanie; Welzel Hilke; Böhm Michael; Nickenig Georg;
Journal:Circulation
PubMed ID:12021231
BACKGROUND: Reactive oxygen species, such as superoxide (O(2)(-)), are involved in the abnormal growth of various cell types. Angiotensin II (Ang II) is one of the most potent inducers of oxidative stress in the vasculature. The molecular events involved in Ang II-induced proliferation of vascular smooth muscle cells (VSMCs) are ... More
Interaction of CED-6/GULP, an adapter protein involved in engulfment of apoptotic cells with CED-1 and CD91/low density lipoprotein receptor-related protein (LRP).
Authors:Su Hua Poo; Nakada-Tsukui Kumiko; Tosello-Trampont Annie-Carole; Li Yonghe; Bu Guojun; Henson Peter M; Ravichandran Kodimangalam S;
Journal:J Biol Chem
PubMed ID:11729193
The prompt clearance of cells undergoing apoptosis is critical during embryonic development, normal tissue turnover, as well as inflammation and autoimmunity. The molecular details of the engulfment of apoptotic cells are not fully understood. ced-6 and its human homologue gulp, encode an adapter protein, whose function in engulfment is highly ... More
Regulation of cortical dendrite development by Slit-Robo interactions.
Authors:Whitford Kristin L; Marillat Valérie; Stein Elke; Goodman Corey S; Tessier-Lavigne Marc; Chédotal Alain; Ghosh Anirvan;
Journal:Neuron
PubMed ID:11779471
Slit proteins have previously been shown to regulate axon guidance, branching, and neural migration. Here we report that, in addition to acting as a chemorepellant for cortical axons, Slit1 regulates dendritic development. Slit1 is expressed in the developing cortex, and exposure to Slit1 leads to increased dendritic growth and branching. ... More
Human cytomegalovirus-encoded interleukin-10 homolog inhibits maturation of dendritic cells and alters their functionality.
Authors:Chang WL, Baumgarth N, Yu D, Barry PA,
Journal:J Virol
PubMed ID:15280480
Interleukin-10 (IL-10) suppresses the maturation and cytokine production of dendritic cells (DCs), key regulators of adaptive immunity, and prevents the activation and polarization of naïve T cells towards protective gamma interferon-producing effectors. We hypothesized that human cytomegalovirus (HCMV) utilizes its viral IL-10 homolog (cmvIL-10) to attenuate DC functionality, thereby subverting ... More
A comparison between different human hepatocyte models reveals profound differences in net glucose production, lipid composition and metabolism in vitro.
Authors:Bonanini F,Singh M,Yang H,Kurek D,Harms AC,Mardinoglu A,Hankemeier T
Journal:Experimental cell research
PubMed ID:38499143
132 total citations

Other products to consider



Share catalog number, name or link

1x1 image pixel for data collection