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PureLink™ Viral RNA/DNA Mini Kit
PureLink™ Viral RNA/DNA Mini Kit
Invitrogen™

PureLink™ Viral RNA/DNA Mini Kit

The PureLink Viral RNA/DNA Mini Kit provides a rapid and efficient method to simultaneously purify viral RNA/DNA from fresh orRead more
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Catalog NumberQuantity
1228005050 Preps
Catalog number 12280050
Price (USD)
364.65
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389.00
Save 24.35 (6%)
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Quantity:
50 Preps
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Price (USD)
364.65
Online Exclusive
389.00
Save 24.35 (6%)
Each
Add to cart
Ask our AI about this Product
The PureLink Viral RNA/DNA Mini Kit provides a rapid and efficient method to simultaneously purify viral RNA/DNA from fresh or frozen cell-free biological fluids (plasma, serum, cerebrospinal fluid) and cell culture supernatants. The kit is specifically designed to isolate high-quality viral nucleic acids from a variety of RNA and DNA viruses within 45 minutes using low elution volumes that allow sensitive downstream analysis. The purified viral RNA/DNA is devoid of proteins and nucleases and is suitable for use in downstream applications that allow viral detection and genotyping.

The PureLink Viral Mini Kit has been used to purify viral dsDNA from orthopoxviruses including cowpox virus, variola virus, monkeypox virus, vaccinia virus, and HERV.

The PureLink Viral RNA/DNA Mini Kit provides the following advantages:
• Rapid and efficient purification of high-quality viral nucleic acid using spin column-based centrifugation with no sample cross-contamination
• Specifically designed to purify viral RNA and DNA from ≤500 μL cell-free samples within 45 min
• Ability to elute viral nucleic acids in low elution volumes of 10–50 μL to allow sensitive downstream analysis

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Column TypeSpin Column
Elution Volume10 to 50 μL
Final Product TypeViral RNA and DNA
For Use With (Application)RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization
High-throughput CompatibilityNot High-throughput Compatible (Manual)
Quantity50 Preps
Sample TypeCells, Cerebrospinal Fluid, Plasma, Serum, Cell Culture Supernatants, Raw and Stabilized Saliva, Nasopharyngeal Viral Transfer Medium (NPVTM)
ScaleMini
Shipping ConditionRoom Temperature
Starting Material Amount≤500 μL
Test Time45 min.
Yield5 μg (Binding capacity)
Isolation TechnologySilica Spin Column
Unit SizeEach
Contents & Storage
• 310 μg Carrier RNA; –20°C
• 32 mL Viral Lysis Buffer L22; room temperature
• 15 mL Wash Buffer WII; room temperature
• 2 x 1.6 mL Proteinase K; room temperature, or 4°C for long-term storage
• 15.5 mL RNase-free Water E3; room temperature
• 50 Viral Spin Columns with Collection Tubes; room temperature
• 2 x 50 Wash Tubes; room temperature
• 50 Recovery Tubes; room temperature

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Frequently asked questions (FAQs)

Are additional wash tubes available for the PureLink Viral RNA/DNA kits if I do not want to use the wash columns twice?

Yes, the Cat. No. for 100 wash tubes is 12282-100.

What is the stability of the Proteinase K in the PureLink Viral RNA/DNA kits?

The Proteinase K solution is stable for 1 year when stored at room temperature. For long-term storage (>1 year) or if room temperature is >25 degrees C, store the Proteinase K solution at 4 degrees C.

What is the difference between serum and plasma?

Plasma is the liquid portion of the blood that is separated from the blood cells by centrifugation. One of the characteristics of plasma is that it clots easily, which is important for hemophiliacs needing a transfusion but is a nuisance in most other applications. By agitating the plasma, one can precipitate the clotting factors as a large clot, and the leftover fluid is called serum. In other words, serum plus clotting factors is plasma, and clotted plasma yields serum.

What is the stability of the carrier RNA in the PureLink Viral RNA/DNA kit, both lyophilized and in solution?

The tRNA is stable for over one year. There was no significant difference in qRT-PCR results with tRNA stored for 10 days at ‘20 degrees C or 42 degrees C. The lyophilized tRNA is stable at ‘20 degrees C, room temperature, 37 degrees C, and 45 degrees C for up to 1 month.

Do I need to use the carrier RNA in the PureLink Viral RNA/DNA kit?

The carrier RNA is tRNA and it may not be necessary. We have tested without the tRNA and obtained excellent results. The tRNA provides a substrate for RNases in the sample to protect the viral RNA until the virus is lysed. You may want to scale down the amount of carrier RNA or leave it out. However, you should validate it in your application to make sure you get the same results as when you include it.

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3182927Certificate of AnalysisJul 03, 202512280050
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Citations & References (8)

Citations & References
Abstract
Surveillance of feral cats for influenza A virus in north central Florida.
Authors:Gordy JT,Jones CA,Rue J,Crawford PC,Levy JK,Stallknecht DE,Tripp RA,Tompkins SM
Journal:Influenza and other respiratory viruses
PubMed ID:22212818
Please cite this paper as: Gordy JT et al. (2012) Surveillance of feral cats for influenza A virus in North Central Florida. Influenza and Other Respiratory Viruses 6(5), 341–347. Background  Transmission of highly pathogenic avian influenza and the recent pandemic H1N1 viruses to domestic cats and other felids creates concern because ... More
Widespread Virus Replication in Alveoli Drives Acute Respiratory Distress Syndrome in Aerosolized H5N1 Influenza Infection of Macaques.
Authors:Wonderlich ER,Swan ZD,Bissel SJ,Hartman AL,Carney JP,O'Malley KJ,Obadan AO,Santos J,Walker R,Sturgeon TJ,Frye LJ Jr,Maiello P,Scanga CA,Bowling JD,Bouwer AL,Duangkhae PA,Wiley CA,Flynn JL,Wang J,Cole KS,Perez DR,Reed DS,Barratt-Boyes SM
Journal:Journal of immunology (Baltimore, Md. : 1950)
PubMed ID:28062701
Human infections with highly pathogenic avian influenza A (H5N1) virus are frequently fatal but the mechanisms of disease remain ill-defined. H5N1 infection is associated with intense production of proinflammatory cytokines, but whether this cytokine storm is the main cause of fatality or is a consequence of extensive virus replication that ... More
Production, concentration and titration of pseudotyped HIV-1-based lentiviral vectors.
Authors:Kutner RH, Zhang XY, Reiser J,
Journal:Nat Protoc
PubMed ID:19300443
'Over the past decade, lentiviral vectors have emerged as powerful tools for transgene delivery. The use of lentiviral vectors has become commonplace and applications in the fields of neuroscience, hematology, developmental biology, stem cell biology and transgenesis are rapidly emerging. Also, lentiviral vectors are at present being explored in the ... More
No association of xenotropic murine leukemia virus-related virus with prostate cancer or chronic fatigue syndrome in Japan.
Authors:Furuta RA, Miyazawa T, Sugiyama T, Kuratsune H, Ikeda Y, Sato E, Misawa N, Nakatomi Y, Sakuma R, Yasui K, Yamaguti K, Hirayama F
Journal:Retrovirology
PubMed ID:21414229
The involvement of xenotropic murine leukemia virus-related virus (XMRV) in prostate cancer (PC) and chronic fatigue syndrome (CFS) is disputed as its reported prevalence ranges from 0% to 25% in PC cases and from 0% to more than 80% in CFS cases. To evaluate the risk of XMRV infection during ... More
The microbial detection array for detection of emerging viruses in clinical samples--a useful panmicrobial diagnostic tool.
Authors:Rosenstierne MW, McLoughlin KS, Olesen ML, Papa A, Gardner SN, Engler O, Plumet S, Mirazimi A, Weidmann M, Niedrig M, Fomsgaard A, Erlandsson L
Journal:
PubMed ID:24963710
Emerging viruses are usually endemic to tropical and sub-tropical regions of the world, but increased global travel, climate change and changes in lifestyle are believed to contribute to the spread of these viruses into new regions. Many of these viruses cause similar disease symptoms as other emerging viruses or common ... More
8 total citations

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