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Gibco™
IMDM
IMDM (Iscove's Modified Dulbecco's Medium) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells.Read more
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| Catalog Number | Quantity |
|---|---|
| 12440079 | 6 x 1000 mL |
| 12440053 | 500 mL |
| 12440061 | 10 x 500 mL |
| 12440046 | 1000 mL |
Catalog number 12440079
Price (USD)
401.65
Online Exclusive
452.00Save 50.35 (11%)
Each
Estimated availability date 27-Jun-2025
Available alternatives
Quantity:
6 x 1000 mL
Price (USD)
401.65
Online Exclusive
452.00Save 50.35 (11%)
Each
IMDM (Iscove's Modified Dulbecco's Medium) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells. We offer a variety of IMDM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This IMDM is modified as follows:
With: L-glutamine, Phenol Red
Without: α-thioglycerol, 2-mercaptoethanol
The complete formulation is available.
Using IMDM
IMDM, a modification of Dulbecco's Modified Eagle Medium, includes selenium as well as additional amino acids and vitamins. In addition, this unique medium lacks iron, with potassium nitrate replacing ferric nitrate. IMDM contains no proteins, lipids, or growth factors. Therefore, IMDM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). IMDM uses a sodium bicarbonate buffer system (3.024 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell LineJurkat, COS-7, and macrophage cells
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Product TypeIMDM (Iscove's Modified Dulbecco's Medium)
Quantity6 x 1000 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormLiquid
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesHigh Glucose, Glutamine, HEPES, Phenol Red, Sodium Pyruvate
Without AdditivesNo α-Thioglycerol, No 2-Mercaptoethanol
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Media Formulations
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Lot #Certificate TypeDateCatalog Number(s)
3159898Certificate of AnalysisJun 21, 202512440046, 12440053
3166059Certificate of AnalysisJun 15, 202512440046, 12440053
3224750Certificate of AnalysisJun 11, 202512440046, 12440053
3205031Certificate of AnalysisMay 30, 202512440046, 12440053
3140543Certificate of AnalysisMay 30, 202512440046, 12440053
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Safety Data Sheets
SDSFrequently asked questions (FAQs)
Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.
Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.
We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.
Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.
Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
This can occur if cells are overly trypsinized. Trypsinize for a shorter time or use less trypsin. Mycoplasma contamination could also cause this problem. Segregate your culture and test for mycoplasma infection. Lastly, check for attachment factors in the medium.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Citations & References (1)
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Citations & References
Abstract
Ex vivo differentiation of cord blood stem cells into megakaryocytes and platelets.
Journal:Methods Mol Biol
PubMed ID:23179834
Megakaryocytes (MK) are hematopoietic cells present in the bone marrow that are responsible for the production and release of platelets in the circulation. Given their very low frequency (<1%), human MK often need to be derived in culture to study their development or to generate sufficient material for biological studies.
1 total citations