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Gibco™
Advanced DMEM
Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.
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| Catalog Number | Quantity |
|---|---|
| 12491023 | 10 x 500 mL |
| 12491015 | 500 mL |
Catalog number 12491023
Price (USD)
427.65
Online Exclusive
478.00Save 50.35 (11%)
Each
In stock
Quantity:
10 x 500 mL
Price (USD)
427.65
Online Exclusive
478.00Save 50.35 (11%)
Each
Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in Advanced DMEM, with no adaptation, include MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero.
This Advanced DMEM is manufactured as follows:
With: High Glucose, Non-essential Amino Acids, Sodium Pyruvate, Phenol Red
Without: L-glutamine
The complete formulation is available.
Using Advanced DMEM
Advanced DMEM is unique from other media due to the addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM requires supplementation with 1–5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell LineMDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Product TypeDMEM (Dulbecco's Modified Eagle Medium)
Quantity10 x 500 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin
FormLiquid
Serum LevelReduced Serum
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesHigh Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)
Without AdditivesNo Glutamine, No HEPES
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Media Formulations
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Lot #Certificate TypeDateCatalog Number(s)
3126849Certificate of AnalysisMay 29, 202512491015
3108044Certificate of AnalysisApr 05, 202512491015
3117080Certificate of AnalysisFeb 23, 202512491015
2715552Certificate of AnalysisJan 29, 202512491015
2809606Certificate of AnalysisJan 28, 202512491015
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SDSCitations & References (2)
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Citations & References
Abstract
Surface functionalization of carbon nanomaterials by self-assembling hydrophobin proteins.
Journal:Biopolymers
PubMed ID:23097233
'Class I fungal hydrophobins are small surface-active proteins that self-assemble to form amphipathic monolayers composed of amyloid-like rodlets. The monolayers are extremely robust and can adsorb onto both hydrophobic and hydrophilic surfaces to reverse their wettability. This adherence is particularly strong for hydrophobic materials. In this report, we show that
Phenotypic changes of adult porcine mesenchymal stem cells induced by prolonged passaging in culture.
Journal:J Cell Physiol
PubMed ID:15880640
'The in vitro culture of porcine bone marrow-derived mesenchymal stem cells (MSCs) was used for the investigation of adult stem cell biology. Isolated porcine MSCs possessed the ability to proliferate extensively in Gibco(R) Advanced DMEM containing 5% fetal bovine serum (FBS). Greater than 40 serial MSC passages and 100 cell
2 total citations