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Gibco™
HBSS, calcium, magnesium, no phenol red
Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation,Read more
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| Catalog Number | Quantity |
|---|---|
| 14025076 | 1000 mL |
| 14025092 | 500 mL |
| 14025134 | 10 x 500 mL |
| 14025126 | 6 x 1000 mL |
Catalog number 14025076
Price (USD)
48.65
Online Exclusive
52.75Save 4.10 (8%)
Each
Quantity:
1000 mL
Price (USD)
48.65
Online Exclusive
52.75Save 4.10 (8%)
Each
Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. Formulations with calcium and magnesium are generally used as transport media, or for reagent preparation.
We offer a variety of Gibco™ HBSS formulations for a range of cell culture applications.
This HBSS is modified as follows:
| With | Without |
| • Calcium | • Phenol Red |
| • Magnesium | • Sodium Pyruvate |
| • Glucose |
The complete formulation is available.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
AdditiveNo Sodium Pyruvate
ClassificationAnimal Origin-Free
Dilution1 X
For Use With (Application)Mammalian Cell Culture
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Osmolality270 - 305 mOsm/kg
Phenol Red IndicatorNo Phenol Red
Product LineGibco™
Quantity1000 mL
Shipping ConditionRoom Temperature
SterilitySterile-filtered
FormLiquid
Product TypeHanks' Balanced Salt Solution (HBSS)
Solution TypeHank's Balanced Salt
pH6.7 - 7.8
Unit SizeEach
Contents & Storage
Storage conditions: 15–30°C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
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Media Formulations
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Certificates
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Lot #Certificate TypeDateCatalog Number(s)
3150761Certificate of AnalysisJun 22, 202514025076, 14025050, 14025092
3148763Certificate of AnalysisJun 11, 202514025076, 14025050, 14025092
3209557Certificate of AnalysisJun 07, 202514025076, 14025050, 14025092
3140604Certificate of AnalysisJun 06, 202514025076, 14025050, 14025092
3150543Certificate of AnalysisMay 11, 202514025076, 14025050, 14025092
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Safety Data Sheets
SDSFrequently asked questions (FAQs)
The recommended storage condition for our HBSS products is at 15-30 degrees C. Generally, storage at 4 degrees C will not impact these products. The only concern would be for the 10x HBSS solutions with calcium and magnesium as these salts might precipitate when stored outside the recommended temperature ranges. As long as you don't see any precipitation upon refrigeration, the product will be fine.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
HBSS (Cat. No. 14025xxx) does not contain HEPES and it needs to be externally supplemented. The complete formulation is available here (https://www.thermofisher.com/us/en/home/technical-resources/media-formulation.153.html).
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Phenol red is a pH indicator which allows you to determine the optimal atmospheric conditions for your cell line during your particular assays. We offer both versions to allow you the option to utilize this indicator in your media.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
No, a saline solution that contains calcium and magnesium should not be used to wash cells before dissociation.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
No, this is not normal and if there is a precipitate in the product, it cannot be used. If the precipitate will not go back into solution, please contact Technical Support at techsupport@thermofisher.com for a replacement.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Citations & References (2)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Production, concentration and titration of pseudotyped HIV-1-based lentiviral vectors.
Journal:Nat Protoc
PubMed ID:19300443
'Over the past decade, lentiviral vectors have emerged as powerful tools for transgene delivery. The use of lentiviral vectors has become commonplace and applications in the fields of neuroscience, hematology, developmental biology, stem cell biology and transgenesis are rapidly emerging. Also, lentiviral vectors are at present being explored in the
Multicolor and electron microscopic imaging of connexin trafficking.
Journal:Science
PubMed ID:11964472
'Recombinant proteins containing tetracysteine tags can be successively labeled in living cells with different colors of biarsenical fluorophores so that older and younger protein molecules can be sharply distinguished by both fluorescence and electron microscopy. Here we used this approach to show that newly synthesized connexin43 was transported predominantly in
2 total citations