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HBSS (10X), no calcium, no magnesium, no phenol red
Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation,Read more
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| Catalog Number | Quantity |
|---|---|
| 14185052 | 500 mL |
Catalog number 14185052
Price (USD)
64.65
Online Exclusive
70.00Save 5.35 (8%)
Each
In stock
Quantity:
500 mL
Price (USD)
64.65
Online Exclusive
70.00Save 5.35 (8%)
Each
Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Formulations without calcium and magnesium are required for rinsing chelators from the culture before cell dissociation. We offer a variety of Gibco™ HBSS formulations for a range of cell culture applications. Find the right formulation using the media selector tool.
This HBSS is modified as follows:
| With | Without |
| • Glucose | • Calcium |
| • Magnesium | |
| • Phenol Red | |
| • Sodium Bicarbonate |
The complete formulation is available.
Concentrated forms of Gibco™ HBSS require sodium bicarbonate addition, pH adjustment, and filtration at the time of preparation (see protocol for details).
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
AdditiveNo Sodium Pyruvate
ClassificationAnimal Origin-Free
Dilution10 X
For Use With (Application)Variety of Cell Culture Applications; Such as Washing Cells Before Dissociation; Transporting Cells or Tissue Samples; Diluting Cells for Counting; and Preparing Reagents
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Osmolality2700 - 3000 mOsm/kg
Phenol Red IndicatorNo Phenol Red
Product LineGibco™
Quantity500 mL
Shipping ConditionRoom Temperature
SterilitySterile-filtered
FormLiquid
Product TypeHanks' Balanced Salt Solution (HBSS)
Solution TypeHank's Balanced Salt
pH5.8 - 6.1
Unit SizeEach
Contents & Storage
Storage conditions: 15-30° C
Shipping conditions: Ambient
Shelf life: 24 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 24 months from date of manufacture
Media Formulations
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Lot #Certificate TypeDateCatalog Number(s)
3205042Certificate of AnalysisJun 26, 202514185052, 14185045
3209713Certificate of AnalysisJun 13, 202514185052, 14185045
3159937Certificate of AnalysisApr 02, 202514185052, 14185045
3126829Certificate of AnalysisMar 30, 202514185052, 14185045
2428788Certificate of AnalysisJan 29, 202514185052
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Frequently asked questions (FAQs)
Yes, we do offer HBSS (10X), no calcium, no magnesium, no phenol red (Cat. No. 14185052).
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
The recommended storage condition for our HBSS products is at 15-30 degrees C. Generally, storage at 4 degrees C will not impact these products. The only concern would be for the 10x HBSS solutions with calcium and magnesium as these salts might precipitate when stored outside the recommended temperature ranges. As long as you don't see any precipitation upon refrigeration, the product will be fine.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Yes, please visit this page for a list of our phenol red-free media (http://tools.thermofisher.com/content/sfs/brochures/Phenol_RedFree_Media_Glance.pdf).
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.