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Gibco™
ACK Lysing Buffer
ACK (Ammonium-Chloride-Potassium) Lysing Buffer is used for the lysis of red blood cells in samples containing white blood cells, suchRead more
| Catalog Number | Quantity |
|---|---|
| A1049201 | 100 mL |
Catalog number A1049201
Price (USD)
27.49
Each
-
Quantity:
100 mL
Price (USD)
27.49
Each
ACK (Ammonium-Chloride-Potassium) Lysing Buffer is used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow. The complete formulation and lysis protocol are available in our Technical Reference Library.
cGMP manufacturing and quality system
ACK Lysing Buffer is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
cGMP manufacturing and quality system
ACK Lysing Buffer is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical Name or MaterialLysing Buffer
Recommended StorageStorage conditions: 15–30°C. Protect from light.
Shipping conditions: ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: ambient
Shelf life: 12 months from date of manufacture
Shelf Life12 Months
SterilitySterile
Physical FormLiquid
Product LineGibco
Quantity100 mL
Unit SizeEach
Media Formulations
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Certificates
Search by lot number or partial lot number
Lot #Certificate TypeDateCatalog Number(s)
3191886Certificate of AnalysisMay 23, 2025A1049201
3150577Certificate of AnalysisMay 09, 2025A1049201
3088726Certificate of AnalysisMar 30, 2025A1049201
3117092Certificate of AnalysisMar 22, 2025A1049201
2451173Certificate of AnalysisJan 29, 2025A1049201
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Frequently asked questions (FAQs)
Alternative products we offer are eBioscience 1X RBC Lysis Buffer (Cat. No. 00-4333-57) or eBioscience 10X RBC Lysis Buffer (Multi-species) (Cat. No. 00-4300-54).
Yes, ACK Lysing Buffer can be used with heparinized blood. The principle of lysis is osmotic treatment.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
A volume of ACK lysing buffer corresponding to 10-20 times the volume of the blood sample is sufficient to lyse red blood cells.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Citations & References (1)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Isolation of carbohydrate-specific CD4(+) T cell clones from mice after stimulation by two model glycoconjugate vaccines.
Journal:Nat Protoc
PubMed ID:23196974
Here we describe how to isolate carbohydrate-specific T cell clones (for which we propose the designation 'Tcarbs') after stimulation by two glycoconjugate vaccines. We describe how to prepare, purify and characterize two model glycoconjugate vaccines that can be used to generate Tcarbs. These glycoconjugate vaccines (GBSIII-OVA and GBSIII-TT) are synthesized
1 total citations