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PNGase F Glycan Cleavage Kit
The PNGase F Glycan Cleavage Kit includes all components necessary to perform the enzymatic removal of almost all N-linked oligosaccharidesRead more
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| Catalog Number | Quantity |
|---|---|
| A39245 | 1 kit |
Catalog number A39245
Price (USD)
371.65
Online Exclusive
394.00Save 22.35 (6%)
Each
In stock
Quantity:
1 kit
Price (USD)
371.65
Online Exclusive
394.00Save 22.35 (6%)
Each
The PNGase F Glycan Cleavage Kit includes all components necessary to perform the enzymatic removal of almost all N-linked oligosaccharides from glycoproteins. The kit includes the PNGase F enzyme, which cleaves N-glycan chains at the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides.
The PNGase F Glycan Cleavage Kit features include:
• ≥95% purity
• Recombinant PNGase F enzyme with no endoglycosidase F1, F2, or F3
• Enzyme leaves N-glycan core oligosaccharides intact and suitable for downstream analysis
• Suitable for use under native or denaturing conditions
The components included in the PNGase F Glycan Cleavage Kit are: 30,000 units of PNGase F (500,000 units/mL) and 1 mL PNGase F 10X buffer.
The PNGase F Glycan Cleavage Kit features include:
• ≥95% purity
• Recombinant PNGase F enzyme with no endoglycosidase F1, F2, or F3
• Enzyme leaves N-glycan core oligosaccharides intact and suitable for downstream analysis
• Suitable for use under native or denaturing conditions
The components included in the PNGase F Glycan Cleavage Kit are: 30,000 units of PNGase F (500,000 units/mL) and 1 mL PNGase F 10X buffer.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormLiquid
Quantity1 kit
Shipping ConditionWet Ice
Product TypePNGase F Glycan Cleavage Kit
Unit SizeEach
Contents & Storage
• 30,000 units PNGase F enzyme, recombinant (500,000 units/mL), store at -20°C
• 1 mL PNGase F 10X Buffer, store at -20°C
• 1 mL PNGase F 10X Buffer, store at -20°C
Have questions about this product? Ask our AI assisted search.
In the PNGase Glycan Cleavage Kit, what happens to the asparagine residue after PNGase F removes the sugar?
In the PNGase Glycan Cleavage Kit, is PNGase F compatible with downstream analysis such as HPLC and mass spectrometry?
In the PNGase Glycan Cleavage Kit, what are the typical reaction conditions for PNGase F, Recombinant?
In the PNGase Glycan Cleavage Kit, how much PNGase F should I use to remove my carbohydrate under native or DTT denaturing conditions?
In the PNGase Glycan Cleavage Kit, can I use PNGase F under native or denaturing conditions?
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Lot #Certificate TypeDateCatalog Number(s)
3231386Certificate of AnalysisJun 10, 2025A39245
3176339Certificate of AnalysisMay 12, 2025A39245
3149996Certificate of AnalysisMar 04, 2025A39245
3124894Certificate of AnalysisJan 15, 2025A39245
3037345Certificate of AnalysisDec 16, 2024A39245
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Safety Data Sheets
SDSFrequently asked questions (FAQs)
Yes, PNGase F enzyme may be used in both native and denaturing conditions
Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.
When the protein is not denatured with SDS, PNGase F has to work harder to reach the cleavage site of the carbohydrate (due to secondary and tertiary protein structures). In these instances, using more enzyme and/or extended incubation times may help. These conditions will need to be determined empirically for your specific protein.
Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.
PNGase F enzyme is stored in 50% glycerol. Therefore, HPLC and/or mass spectrometry is not compatible due to the fact that glycerol is not tolerated in instruments used for these applications.
Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.
Typical reaction conditions are as follows:
Optimal incubation times and enzyme concentrations must be determined empirically for a particular substrate.
1. Combine 1-20 µg of glycoprotein, 1 µL of 10X denaturing buffer (5% SDS, 400 mM DTT) and H2O (if necessary) to make a 10 µL total reaction volume.
2. Denature glycoprotein by heating reaction at 100 degrees C for 10 min.
3. Make a total reaction volume of 20 µL by adding 2 µL PNGaseF 10X Buffer, 1-5 µL PNGaseF enzyme, 2 µL 10% NP-40 and H2O.
4. Incubate reaction at 37 degrees C for 1 hr.
Note: Reactions may be scaled-up linearly to accommodate larger reaction volumes.
Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.
As PNGase F is a glycoamidase, the asparagine gets converted into aspartic acid.
Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.