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Citations & References (7)
Thermo Scientific™
EasyPep™ MS Sample Prep Kits
Generate high quality proteomic samples for MS analysis in less than four hours using optimized EasyPep MS Sample Prep kits.
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| Catalog Number | Description |
|---|---|
| A45734 | Maxi Sample Prep Kit |
| A40006 | Mini MS Sample Prep Kit |
| A57864 | Micro MS Sample Prep Kit |
| A45733 | MS Sample Prep Kit |
| A45735 | Lysis Buffer |
| A57865 | Peptide Clean-up Plate |
Catalog number A45734
Price (KRW)
2,451,000
Online offer
Ends: 30-Jun-2025
2,883,000Save 432,000 (15%)
Each
-
Description:
Maxi Sample Prep Kit
Price (KRW)
2,451,000
Online offer
Ends: 30-Jun-2025
2,883,000Save 432,000 (15%)
Each
EasyPep Mini MS Sample Prep Kits enable efficient and reproducible processing of cultured mammalian cells and tissues for proteomic MS analysis. The kit contains pre-formulated buffers, MS-grade enzyme mix, peptide clean-up columns, and an optimized protocol to generate MS-compatible peptide samples in less than three hours. Kits are optimized to process protein samples from 10–100 μg with a high yield of MS-ready peptides.
• Complete—includes pre-formulated reagents for lysis through digestion, peptide clean-up columns, and an optimized protocol for up to 20 samples
• Optimized—streamlined protocol and reagents minimize the number of steps and time time needed to process samples
• Flexible—reagents and protocol have been verified using cells, plasma, and tissue samples for 10 to 100 μg samples
• Time-saving—sample processing time reduced from more than one day to less than three hours
• Compatible—final preparation is ready for direct MS analysis and other downstream applications, including TMT labeling
The sample preparation of peptides for MS analysis is complex, with numerous steps and varied protocols, resulting in sample variability in both quality and quantity. This kit has been designed to improve reproducibility while saving hands-on and processing time. The number of steps and processing time have been reduced through the addition of Universal Nuclease to reduce viscosity from nucleic acids (replacing sonication), a rapid 'one pot' reduction/alkylation solution for cysteine modification (carbamidomethylation, + 57.02), and a trypsin/Lys-C protease mix for more complete digestion. In addition, the kit includes Peptide Clean-up columns and buffers to prepare detergent-free peptide samples for MS analysis. The optimized reagents and protocol produce high-quality peptides that are compatible with TMT labeling and other downstream applications, or are ready for MS analysis.
• Optimized—streamlined protocol and reagents minimize the number of steps and time time needed to process samples
• Flexible—reagents and protocol have been verified using cells, plasma, and tissue samples for 10 to 100 μg samples
• Time-saving—sample processing time reduced from more than one day to less than three hours
• Compatible—final preparation is ready for direct MS analysis and other downstream applications, including TMT labeling
The sample preparation of peptides for MS analysis is complex, with numerous steps and varied protocols, resulting in sample variability in both quality and quantity. This kit has been designed to improve reproducibility while saving hands-on and processing time. The number of steps and processing time have been reduced through the addition of Universal Nuclease to reduce viscosity from nucleic acids (replacing sonication), a rapid 'one pot' reduction/alkylation solution for cysteine modification (carbamidomethylation, + 57.02), and a trypsin/Lys-C protease mix for more complete digestion. In addition, the kit includes Peptide Clean-up columns and buffers to prepare detergent-free peptide samples for MS analysis. The optimized reagents and protocol produce high-quality peptides that are compatible with TMT labeling and other downstream applications, or are ready for MS analysis.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionMaxi Sample Prep Kit
Final Product TypePeptides
For Use With (Equipment)Mass Spectrometer
Label or DyeUnlabeled
Quantity8 Reactions
Shipping ConditionWet Ice
Workflow StepProtein Digestion
Detection MethodMass Spectrometry
Product LineEasyPep™
Product TypeSample Preparation Kit
Starting MaterialProtein Samples
Unit SizeEach
Contents & Storage
- Lysis Solution, 25 mL, store at 4°C
- Universal Nuclease, 25 kU, store at -20°C
- Reduction Solution, 7 mL, store at 4°C
- Alkylation Solution, 7 mL, store at 4°C
- Enzyme Reconstititution solution, 7 mL, store at 4°C
- Pierce™ Trypsin/Lys-C Protease Mix, MS Grade, 8 x 100 μg, store at -20°C
- Digestion Stop Solution, 7 mL, store at 4°C
- Peptide Clean-Up Columns, 8 Ea., store at 4°C
- Wash Solution A, 40 mL, store at 4°C
- Wash Solution B, 3 x 27 mL, store at 4°C
- Elution Solution, 2 x 20 mL, store at 4°C
- Low Protein Binding Collection Tubes, 2 mL, 40 Ea., store at 4°C
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Lot #Certificate TypeDateCatalog Number(s)
3216038Certificate of AnalysisJun 27, 2025A45735
3214430ACertificate of AnalysisJun 25, 2025A40006
3214430Certificate of AnalysisJun 25, 2025A40006
3214844ACertificate of AnalysisJun 25, 2025A45733
3214844Certificate of AnalysisJun 25, 2025A45733
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Safety Data Sheets
SDSScientific Resources
Product Information
Frequently asked questions (FAQs)
As per protocol, peptide samples need to be dried down prior to LC-MS analysis. This step can be performed using vacuum pumps/dry-down devices without any harm caused to the device by the components present in the elution buffer within the EasyPep MS Sample Prep Kits.
Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.
High sample viscosity after lysis is due to release of DNA from the nucleus. Sonication or addition of a nuclease such as the Pierce Universal Nuclease (Cat. No. 88700, 88701, or 88702) can be used to degrade DNA and reduce sample viscosity.
Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.
Add 0.5 mm diameter glass beads to the sample pelleted in Easy Pep Lysis Solution and proceed as recommended in the Easy Pep sample prep protocol. Vortex vigorously for few minutes, spin at 16,000 x g for 5 mins, and extract the supernatant for protein assay and further downstream sample preparation.
Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.
Add 1-2 µL of 50 mg/mL Lysozyme Solution (Cat. No. 90082) to 200 µL of the EasyPep lysis buffer provided in the kit and proceed with the sample prep. Add 200 µL of the prepared lysis solution to 5 X 10E8 bacterial cells (E. coli cells) (OD600 equal to 1, corresponding to 1 X 10E8 CFUs). Lyse by pipetting the sample repeatedly and centrifuge at 16,000 x g for 5 mins. Alternatively, E. coli cells can be lysed in lysis buffer using bead beating or sonication instead of lysozyme. After lysis, dilute samples to 1 mg/mL using lysis buffer, proceed with the EasyPep protocol for reduction, alkylation, digestion, and clean up as described in the product manual.
Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.
High Select Top14 Abundant Protein Depletion Mini Spin columns (Cat. Nos. A36369, A36370) can be used to deplete top 14 abundant plasma/serum proteins. The depletion of highly abundant proteins from the sample enables the detection and identification of low abundant proteins. The depleted samples can be processed with EasyPep MS sample prep kit to generate digested plasma/serum samples. The clean digested samples can be processed by nanoLC-MS/MS analysis for discovery experiments or nanoLC-PRM/MS analysis for targeted experiments.
Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.
Citations & References (7)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Discovery of Orally Available and Brain Penetrant AEP Inhibitors.
Journal:Journal of medicinal chemistry
PubMed ID:38090813
Alzheimer's Disease (AD) is the most widespread form of dementia, with one of the pathological hallmarks being the formation of neurofibrillary tangles (NFTs). These tangles consist of phosphorylated Tau fragments. Asparagine endopeptidase (AEP) is a key Tau cleaving enzyme that generates aggregation-prone Tau fragments. Inhibition of AEP to reduce the
Development of a First-in-Class RIPK1 Degrader to Enhance Antitumor Immunity.
Journal:Research square
PubMed ID:38659866
The scaffolding function of receptor interacting protein kinase 1 (RIPK1) confers intrinsic and extrinsic resistance to immune checkpoint blockades (ICBs) and has emerged as a promising target for improving cancer immunotherapies. To address the challenge posed by a poorly defined binding pocket within the intermediate domain, we harnessed proteolysis targeting
Proteomic Characterization of Transfusable Blood Components: Fresh Frozen Plasma, Cryoprecipitate, and Derived Extracellular Vesicles via Data-Independent Mass Spectrometry.
Journal:Journal of proteome research
PubMed ID:39254217
Extracellular vesicles (EVs) are a heterogeneous collection of particles that play a crucial role in cell-to-cell communication, primarily due to their ability to transport molecules, such as proteins. Thus, profiling EV-associated proteins offers insight into their biological effects. EVs can be isolated from various biological fluids, including donor blood components
Targeted sulfur(VI) fluoride exchange-mediated covalent modification of a tyrosine residue in the catalytic pocket of tyrosyl-DNA phosphodiesterase 1.
Journal:Communications chemistry
PubMed ID:39284936
Developing effective inhibitors of the DNA repair enzyme tyrosyl-DNA phosphodiesterase 1 (TDP1) has been challenging because of the enzyme shallow catalytic pocket and non-specific substrate binding interactions. Recently, we discovered a quinolone-binding hot spot in TDP1's active site proximal to the evolutionary conserved Y204 and F259 residues that position DNA.
Proteomic and N-glycomic comparison of synthetic and bovine whey proteins and their effect on human gut microbiomes.
Journal:bioRxiv : the preprint server for biology
PubMed ID:39345577
Advances in food production systems and customer acceptance have led to the commercial launch of dietary proteins produced via modern biotechnological approaches as alternatives to traditional agricultural sources. At the same time, a deeper understanding of how dietary components interact with the gut microbiome has highlighted the importance of understanding
7 total citations