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Maxima H Minus Reverse Transcriptase (200 U/μL)
Thermo Scientific Maxima H Minus Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possessesRead more
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| Catalog Number | Quantity |
|---|---|
| EP0752 | 10,000 Units |
| EP0751 | 2,000 Units |
| EP0753 | 4 x 10,000 Units |
Catalog number EP0752
Price (EUR)
270,00
Each
-
Quantity:
10,000 Units
Price (EUR)
270,00
Each
Thermo Scientific Maxima H Minus Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity but lacks RNase H activity due to mutation in RNase H domain of M-MuLV RT. The engineered enzyme features dramatically improved thermostability, 50X higher processivity, robustness, and increased synthesis rate compared to wild type M-MuLV RT.
The eliminated RNase H activity enables the enzyme to produce very long RNA transcripts up to 20 kb. Due to its high thermostability, the enzyme maintains full activity during the entire reverse transcription reaction and generates high yields of cDNA. The reaction temperature can be increased to 65°C for efficient transcription of RNA regions with a high secondary structure or to improve specificity using gene specific primers. The extremely high processivity of Maxima H Minus enzyme results in increased resistance to common reaction inhibitors, such as guanidine, formamide, and ethanol.
Features of Maxima H Minus Reverse Transcriptase include:
• Thermostable—90% active after incubation at 50°C for 60 minutes in a reaction mixture
• Active up to 65°C
• High yields of full-length cDNA up to 20 kb
• High sensitivity—reproducible cDNA synthesis from a wide range of starting total RNA amounts (1 pg to 5 μg)
• Efficient—complete cDNA synthesis in 15 to 30 minutes
• Increased resistance to common reaction inhibitors
• Incorporates modified nucleotides
Applications
• First strand cDNA synthesis for RT-PCR and RT-qPCR
• Reverse transcription at elevated temperatures to reduce effects of secondary structure
• Synthesis of cDNA for cloning and expression
• Generation of labeled cDNA probes for microarrays
• Analysis of RNA by primer extension
Related products
• Maxima H Minus Reverse Transcriptase
• Maxima H Minus First Strand cDNA Synthesis Kit
• Maxima H Minus First Strand cDNA Synthesis Kit, with dsDNase
• Maxima H Minus cDNA Synthesis Master Mix
• Maxima H Minus cDNA Synthesis Master Mix, with dsDNase
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration200 U/μL
Final Product TypeFirst-Strand cDNA
FormatStand-alone Enzyme
Optimal Reaction Temperature50°C to 55°C
Quantity10,000 Units
Reaction FormatSeparate components
Reagent TypeReverse Transcription
Reverse TranscriptaseMaxima H Minus
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
Size (Final Product)Up to 20 kb
Starting MaterialRNA
TechniqueReverse Transcription
GC-Rich PCR PerformanceHigh
Reaction Speed15 to 30 min.
Unit SizeEach
Contents & Storage
• Maxima H Minus Reverse Transcriptase, 1 x 10,000 units (200 U/μL)
• 5X RT Buffer
Store at –20°C.
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Figures
High yields of cDNA over a broad temperature range
High thermostability of Maxima and Maxima H Minus Reverse Transcriptases
Reduced effect of reaction inhibitors
Amplification of targets up to 20 kb in two step RT-PCR
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Certificates
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Lot #Certificate TypeDateCatalog Number(s)
3266753Certificate of AnalysisJun 23, 2025EP0752
3259988Certificate of AnalysisJun 13, 2025EP0753
3249457Certificate of AnalysisMay 29, 2025EP0751
3249796Certificate of AnalysisMay 29, 2025EP0752
3247444Certificate of AnalysisMay 27, 2025EP0753
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Safety Data Sheets
SDSFrequently asked questions (FAQs)
It is generally beneficial to minimize RNase H activity when aiming to produce long transcripts for cDNA cloning. RNase H degrades RNA from RNA-DNA duplexes, which can result in truncated cDNA during reverse transcription of long mRNA. It is also recommended to use RNase H-minus RTs for template-independent addition of C nucleotides. In contrast, reverse transcriptases with intrinsic RNase H activity are often favored in qPCR applications.
All Thermo Scientific reverse transcriptases possess intrinsic TdT activity although at varying degrees depending upon the reaction conditions. For addition of template-independent C nucleotides (as for SMART and RACE experiments), this specific TdT activity can be induced by Mn2+. We would recommend Maxima H- or RevertAid H- minus RTs for this purpose.
cDNA synthesis at higher temperatures ensures successful transcription of RNA with high levels of secondary structure, reducing issues of primer access to template. Therefore, we do recommend to use RT enzymes with high thermostability, e.g. Maxima and Maxima H Minus Reverse Transcriptases, which provide higher yields of full-length cDNA, better sensitivity, and successful transcription of GC-rich templates.
No, this product is always provided with the buffer.
Trace amounts of reagents used in RNA purification protocols may remain in solution and inhibit first-strand synthesis, e.g., SDS, EDTA, guanidine salts, phosphate, pyrophosphate, polyamines, spermidine. To remove trace contaminants, we recommend re-precipitating the RNA with ethanol and washing the pellet with 75% ethanol, or re-purifying the RNA.