Search Thermo Fisher Scientific
Certificates
SDS
Citations & References (372)
Invitrogen™
FM™ 1-43 Dye (N-(3-Triethylammoniumpropyl)-4-(4-(Dibutylamino) Styryl) Pyridinium Dibromide)
FM 1-43 membrane probe is an excellent reagent both for identifying actively firing neurons and for investigating the mechanisms ofRead more
PromotionPromo code:P5906365
Don't miss out: buy 3, only pay for 2
PromotionPromo code:RPUZZ25
Stock up on essentials to piece your discovery together
Have Questions?
Change view
| Catalog Number | Quantity |
|---|---|
| T35356 | 10 x 100μg |
| T3163 | 1mg |
Catalog number T35356
Price (USD)
616.65
Online Exclusive
636.00Save 19.35 (3%)
10 x 100 µg
In stock
Quantity:
10 x 100μg
Price (USD)
616.65
Online Exclusive
636.00Save 19.35 (3%)
10 x 100 µg
FM 1-43 membrane probe is an excellent reagent both for identifying actively firing neurons and for investigating the mechanisms of activity-dependent vesicle cycling. This water-soluble dye, which is nontoxic to cells and virtually nonfluorescent in aqueous medium, is believed to insert into the outer leaflet of the cell membrane where it becomes intensely fluorescent. In a neuron that is actively releasing neurotransmitters, the dye becomes internalized within the recycled synaptic vesicles and the nerve terminals become brightly stained. The nonspecific staining of cell-surface membranes can simply be washed off prior to viewing. FM 1-43 membrane probe is also available as 1 mg in a single vial (T-3163). FM 1-43FX membrane probe (F-35355), an analog of FM 1-43 membrane probe that can be fixed in place using aldehyde-based fixatives, is also available.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorRed
Detection MethodFluorescence
For Use With (Equipment)Fluorescence Microscope
Product LineFM™
Quantity10 x 100μg
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeDye
SubCellular LocalizationPlasma Membrane
Unit Size10 x 100 µg
Contents & Storage
Store at room temperature and protect from light.
Have questions about this product? Ask our AI assisted search.
I want to study endosome trafficking using FM 4-64 dye. Will the label be retained after fixation? And can I label cells that have already been fixed?
I want to study endosomes trafficking using FM 4-64. Will the label be retained after fixation? And can I label already-fixed cells?
The product sheet for FM 1-43 Dye states that the maximum for excitation is 510 nm and emissions is 626 nm, but the spectrum on the product page shows a peak at 470 nm and 580 nm. Can you clarify what the excitation and emission maxima is for this dye?
This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our
Privacy Notice.
Figures
Feline mesenteric Pacinian corpuscle labeled with FM® 1-43.
Live nerve terminals of motorneurons that innervate a rat lumbrical muscle stained with FM® 1-43.
Chemical Structure
Customers who viewed this item also viewed
Documents & Downloads
Certificates
Search by lot number or partial lot number
Lot #Certificate TypeDateCatalog Number(s)
3167961Certificate of AnalysisJun 17, 2025T3163
3049995Certificate of AnalysisSep 24, 2024T35356
2856060Certificate of AnalysisFeb 22, 2024T3163
2615846Certificate of AnalysisMay 26, 2023T35356
2570384Certificate of AnalysisApr 24, 2023T3163
5 results displayed, search above for a specific certificate
Safety Data Sheets
SDSProduct Information
Frequently asked questions (FAQs)
No. For that you would need the FM 4-64FX version. The non-FX version will not be retained upon fixation, leading to loss of much of the stain and an increase in background. The FX version will be retained using an aldehyde-based fixative. Cells that are already fixed will be stained throughout the cell and the signal will not be localized; it is recommended to stain live cells and then fix.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
No. For that, you would need the FM 4-64FX version. The non-FX version will be lost, leading to loss of much of the specific label and a vast increase in background labeling. The FX version will be fixed in place with formaldehyde. Cells that have been fixed already will not label correctly, so you will need to label the cells live and then fix.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
FM lipophilic styryl dyes are known to shift their spectral properties upon binding to membranes. These dyes exhibit an appreciable solvent and environmental dependency in both free and membrane-bound forms. The emissions of the membrane-bound dye shifts to a shorter wavelength compared to the emission in the solvent.
In solvent, FM 1-43 Dye (Cat. Nos. T35356, T3163, F35355, F10317) absorbs in the range of fluorescein (~490 nm) and initially emits at around 626-636 nm. However, upon membrane integration, it exhibits emission in the 565-590 nm range. Because this dye exhibits a large Stokes shift, it does not perfectly fit into a green or orange fluorophore profile, but is rather a yellow-fluorescent dye, and may require a long-pass emission filter.
Note: FM 1-43 Dye is often used with typical fluorescein or GFP optical filter sets for biological imaging but is poorly excited through tetramethylrhodamine filters.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Citations & References (372)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Clathrin-mediated endocytosis near active zones in snake motor boutons.
Journal:The Journal of neuroscience : the official journal of the Society for Neuroscience
PubMed ID:11050119
We have used the activity-dependent probe FM1-43 with electron microscopy (EM) to examine endocytosis at the vertebrate nerveâmuscle synapse. Preparations were fixed after very brief neural stimulation at reduced temperature, and internalized FM1-43 was photoconverted into an electron-dense reaction product. To locate the reaction product, we reconstructed computer renderings of
Properties of fast endocytosis at hippocampal synapses.
Journal:Philosophical transactions of the Royal Society of London. Series B, Biological sciences
PubMed ID:10212482
Regulation of synaptic transmission is a widespread means for dynamic alterations in nervous system function. In several cases, this regulation targets vesicular recycling in presynaptic terminals and may result in substantial changes in efficiency of synaptic transmission. Traditionally, experimental accessibility of the synaptic vesicle cycle in central neuronal synapses has
Endosome fusion and microtubule-based dynamics in the early endocytic pathway of dictyostelium.
Journal:Traffic
PubMed ID:12383345
Dictyostelium amoebae, like mammalian macrophages, take up fluid by macropinocytosis. The present study used fluorescent fluid-phase markers and GFP-labeled microtubules to visualize the uptake, dynamics, and fusion of early endosomes in Dictyostelium. Consecutive labeling with two fluorescent fluid-phase markers demonstrated that within the first few minutes after uptake, new macropinosomes
Reticulated lipid probe fluorescence reveals MDCK cell apical membrane topography.
Journal:Biophys J
PubMed ID:11806917
High spatial resolution confocal microscopy of young MDCK cells stained with the lipophilic probe 1,1'-dihexadecyl-3,3,3',3'- tetramethylindocarbocyanine perchlorate (DiIC(16)) revealed a reticulated fluorescence pattern on the apical membrane. DiIC(16) was delivered as crystals to live cells to minimize possible solvent perturbations of the membrane lipids. The ratio of the integrated fluorescence
Visualization of changes in presynaptic function during long-term synaptic plasticity.
Journal:Nat Neurosci
PubMed ID:11426227
Controversy exists regarding the site of modification of synaptic transmission during long-term plasticity in the mammalian hippocampus. Here we used a fluorescent marker of presynaptic activity, FM 1-43, to directly image changes in presynaptic function during both short-term and long-term forms of plasticity at presynaptic boutons of CA3-CA1 excitatory synapses
372 total citations