Tinción Dil (perclorato de 1,1'-dioctadecil-3,3,3',3'-tetrametilindocarbocianina ('Dil'; DiIC₁₈(3)))

Tinción Dil (perclorato de 1,1'-dioctadecil-3,3,3',3'-tetrametilindocarbocianina ('Dil'; DiIC<sub>18</sub>(3)))

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Tinción Dil (perclorato de 1,1'-dioctadecil-3,3,3',3'-tetrametilindocarbocianina ('Dil'; DiIC₁₈(3)))

Name: Tinción Dil (perclorato de 1,1'-dioctadecil-3,3,3',3'-tetrametilindocarbocianina ('Dil'; DiIC 18 (3)))
SKU: D282

DiI es una tinción de membrana lipofílica que se expande lateralmente para teñir toda la célula. Es ligeramente fluorescente hastaMás información

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Número de catálogo	Cantidad
D282	100 mg, 100 mg
D3911	25mg, 25mg

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Número de catálogo D282

Precio (USD)

Cantidad:

100 mg, 100 mg

DiI es una tinción de membrana lipofílica que se expande lateralmente para teñir toda la célula. Es ligeramente fluorescente hasta que se incorpora en las membranas. Este colorante rojo/aanaranjado fluorescente, que es espectralmente similar a la tetrametilrodamina, suele utilizarse como un trazador a largo plazo para células neuronales y de otros tipos. DiI también está disponible en forma de solución (V-22885), pasta (N-22880) o sólido (D-282).

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

ColorRojo-naranja, Rojo-naranja

Método de detecciónFluorescente, Fluorescente

Emisión565 nm

Intervalo de longitud de onda de excitación549 nm

Para utilizar con (equipo)Microscopio de fluorescencia, Microscopio de fluorescencia

Cantidad100 mg, 100 mg

Condiciones de envíoTemperatura ambiente

Tipo de productoTinción dil

SubCellular LocalizationMembranas celulares y lípidos, Lipids

Unit SizeEach

Contenido y almacenamiento

Almacenar a temperatura ambiente y proteger de la luz.

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Preguntas frecuentes

I'm labeling live cells with Vybrant DiI or DiD lipophilic cyanine dyes. DiI gives a nice even membrane labeling, but DiD is more "spotty". What can be done?

This is expected. DiD (which is far-red fluorescent) is never as uniform as DiI (which is orange fluorescent). If uniformity is desired, try increasing the label time and concentration, but it still isn't likely to be as uniform as DiI. CellMask Deep Red Plasma Membrane stain is much more uniform and is about the same wavelength as DiD. However, if you intend to do cell tracking over days, CellMask stain has not been tried for that application.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to perform a cell fusion assay, where one cell line is labeled with one color and the other cell line with another color, and combine with a nucleic acid stain. What do you recommend?

A typical method is to label one cell line with orange fluorescent DiI C₁₈ and the other cell line with green fluorescent DiO C₁₈. These orange and green lipophilic cyanine dyes will stain the membranes of cells. Cells that fuse will then have both dyes, yielding a yellow color (when images are overlaid or cells are imaged in a dual-bandpass filter). These live cells can then be labeled with Hoechst 33342 (a cell-permeant blue DNA stain comparable in wavelength to DAPI), but only as an endpoint just before imaging (since DNA stains can interrupt DNA function).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to look at live cell morphology deformation over the course of a few hours. What sort of membrane dye would be useful for this?

Lipophilic cyanine dyes, such as DiI (Cat. No. D282), DiO (Cat. No. D275), DiD (Cat. No. D7757) or DiR (Cat. No. D12731), are commonly used. The longer the alkyl chain on the dye, the better the retention in lipophilic environments.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Why do I lose all signal from my neuronal tracer when I do a methanol fixation on my cells?

If the tracer you chose is a lipophilic dye and fix with methanol, the lipids are lost with the methanol. If you have to use methanol fixation then choose a tracer that will covalently bind to proteins in the neurons.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I stained my cells with a lipophilic cyanine dye, like DiI, but the signal was lost when I tried to follow up with antibody labeling. Why?

Since these dyes insert into lipid membranes, any disruption of the membranes leads to loss of the dye. This includes permeabilization with detergents like Triton X-100 or organic solvents like methanol. Permeabilization is necessary for intracellular antibody labeling, leading to loss of the dye. Instead, a reactive dye such as CFDA SE should be used to allow for covalent attachment to cellular components, thus providing for better retention upon fixation and permeabilization.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all Tinción Dil (perclorato de 1,1'-dioctadecil-3,3,3',3'-tetrametilindocarbocianina ('Dil'; DiIC₁₈(3))) FAQs

Figuras

Newborn mouse cochlea. DiI.

Chemical Structure

Neurons in ventrobasal nucleus of newborn mouse thalamus. DiI, DiD.

Espectros de fluorescencia

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Documentos y descargas

Certificados

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N.º de loteCertificate TypeDateCatalog Number(s)

3192590Certificate of Analysis13 jul 2025D282

3154627Certificate of Analysis18 may 2025D3911

3135809Certificate of Analysis30 mar 2025D282

3096414Certificate of Analysis18 nov 2024D3911

3096413Certificate of Analysis06 nov 2024D282

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Hojas de datos de seguridad

SDS

Product Information

Lipophilic Tracers — DiI, DiO, DiD, DiA and DiR

Manual de Molecular Probes®

Probes for the Endoplasmic Reticulum and Golgi Apparatus—Section 12.4

Dialkylcarbocyanine and Dialkylaminostyryl Probes—Section 13.4

Tracers for Membrane Labeling—Section 14.4

Citations & References (1179)

Search citations by name, author, journal title or abstract text

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Citations & References

Abstract

Using fluorescent dyes for fate mapping, lineage analysis, and axon tracing in the chick embryo.

Authors:Clarke JD

Journal:Methods in molecular biology (Clifton, N.J.)

PubMed ID:19030810

Angiotensin II induces LOX-1, the human endothelial receptor for oxidized low-density lipoprotein.

Authors:Morawietz H,Rueckschloss U,Niemann B,Duerrschmidt N,Galle J,Hakim K,Zerkowski HR,Sawamura T,Holtz J

Journal:Circulation

PubMed ID:10468518

Neural transplant staining with DiI and vital imaging by 2-photon laser-scanning microscopy.

Authors:Potter SM, Pine J, Fraser SE

Journal:Scanning Microsc Suppl

PubMed ID:9601539

We are developing a multielectrode silicon "neuroprobe" for maintaining a long-term, specific, two-way electrical interface with nervous tissue. Our approach involves trapping a neuron (from an embryonic rat hippocampus) in a small well with a stimulation/recording electrode at its base. The well is covered with a grillwork through which the ... More

Cultured postnatal rat septohippocampal neurons change intracellular calcium in response to ethanol and nerve growth factor.

Authors:Webb B, Suarez SS, Heaton MB, Walker DW

Journal:Brain Res

PubMed ID:9459553

Ethanol exposure affects cellular mechanisms involved in the regulation of calcium (Ca2+) homeostasis. Neurotrophins, such as nerve growth factor (NGF), stabilize intracellular Ca2+([Ca2+]i) during a variety of neurotoxic insults. In this study, changes in [Ca2+]i during treatment with ethanol and NGF were measured at the cell body of neurons using ... More

Evaluation of a flow cytometric fluorescence quenching assay of phagocytosis of sensitized sheep erythrocytes by polymorphonuclear leukocytes.

Authors:Van Amersfoort ES, Van Strijp JA

Journal:Cytometry

PubMed ID:7875036

A number of reports have been published describing phagocytosis assays for flow cytometric analysis. In some of these, the fluorescence quenching technique has been used to discriminate between adherent and ingested particles. In this report, we have evaluated the efficacy of a quantitative fluorescence quenching technique with crystal violet and ... More

1179 total citations

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Tinción Dil (perclorato de 1,1'-dioctadecil-3,3,3',3'-tetrametilindocarbocianina ('Dil'; DiIC18(3)))

Preguntas frecuentes

I'm labeling live cells with Vybrant DiI or DiD lipophilic cyanine dyes. DiI gives a nice even membrane labeling, but DiD is more "spotty". What can be done?

I want to perform a cell fusion assay, where one cell line is labeled with one color and the other cell line with another color, and combine with a nucleic acid stain. What do you recommend?

I need to look at live cell morphology deformation over the course of a few hours. What sort of membrane dye would be useful for this?

Why do I lose all signal from my neuronal tracer when I do a methanol fixation on my cells?

I stained my cells with a lipophilic cyanine dye, like DiI, but the signal was lost when I tried to follow up with antibody labeling. Why?

Figuras

Espectros de fluorescencia

Customers who viewed this item also viewed

Documentos y descargas

Certificados

Hojas de datos de seguridad

Product Information

Manual de Molecular Probes®

Citations & References (1179)

Other products to consider

Tinción Dil (perclorato de 1,1'-dioctadecil-3,3,3',3'-tetrametilindocarbocianina ('Dil'; DiIC₁₈(3)))