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CTS™ TrypLE™ Select Enzyme
Gibco CTS TrypLE Select is a recombinant enzyme capable of dissociating a wide range of adherent mammalian cells. CTS TrypLERead more
| Catalog Number | Quantity |
|---|---|
| A4738001 | 1 L |
Catalog number A4738001
Price (CNY)
9,003.00
Each
In stock
Quantity:
1 L
Price (CNY)
9,003.00
Each
Gibco CTS TrypLE Select is a recombinant enzyme capable of dissociating a wide range of adherent mammalian cells. CTS TrypLE Select Enzyme is derived from microbial fermentation and provides an animal origin free (AOF) alternative to porcine trypsin.
Gibco CTS products are designed for use in manufacturing of cell or gene products, supporting your transition from research to clinical applications.
Compared to other dissociation reagents, CTS TrypLE Select Enzyme is:
• Animal origin-free (AOF)—free of animal-derived components and formulated on dedicated animal origin-free equipment. CTS TrypLE Select Enzyme reduces risk of viral or prion contamination in your clinical manufacturing.
• Gentle on cells—CTS TrypLE Select Enzyme's exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.
• Room temperature-stable—Product remains stable at room temperature or 2–8°C, making storage and handling easy and convenient.
• Easy to use—CTS TrypLE Select substitutes directly into existing protocols, with no inactivation required avoiding the need for trypsin inhibitors, such as FBS.
The product is provided as a 1X stock solution, formulated in DPBS with 1 mM EDTA.
Closed systems compatible
The 1-liter size of CTS TrypLE Select Enzyme is provided in bioprocess container format with the chamber constructed from Aegis 5-14 film. Sterile, weldable C-Flex tubing with female luer lock provides versatile connection options for integrating into a closed system manufacturing process.
CTS quality
The Gibco CTS product line enables you to reduce your burden in qualifying reagents during your transition from research applications to clinical applications.
Products are manufactured at a site that uses methods and controls that conform to cGMP for medical devices, 21 CFR Part 820. Our FDA-registered manufacturing sites are ISO 13485- and ISO 9001–certified. Gibco CTS products follow USP 'ancillary materials for cell, gene, and tissue-engineered products' within the responsibilities applicable to a supplier.
Gibco CTS products are of high quality and are supplied with harmonized documentation such as certificates of analysis, certificates of origin, and convenient access to our drug master file (DMF).
Specifications
Concentration1X
For Use With (Application)Cell and Gene Therapy Research, Development, and Manufacturing
FormatBioProcess Container
Green FeaturesSustainable Packaging
Manufacturing QualityISO 13485, MDSAP, FDA-registered, 21 CFR 820
Quantity1 L
Shelf Life12 months from date of manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormLiquid
Product TypeCell Culture Dissociation Reagent
SterilitySterile-filtered
Without AdditivesNo Phenol Red
Unit SizeEach
Contents & Storage
Store at 15–30°C.
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Lot #Certificate TypeDateCatalog Number(s)
3135594Certificate of AnalysisApr 11, 2025A4738001
2366904Certificate of AnalysisJan 29, 2025A4738001
2854413Certificate of AnalysisJan 28, 2025A4738001
2854411Certificate of AnalysisJan 28, 2025A4738001
2927512Certificate of AnalysisJan 25, 2025A4738001
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Safety Data Sheets
SDSScientific Resources
Product Information
Frequently asked questions (FAQs)
The Gibco Cell Therapy Systems (CTS) portfolio of cell and gene therapy products are GMP manufactured, safety tested, and backed by regulatory documentation to support your transition from discovery through clinical and commercial manufacturing. Through our CTS solutions, we are committed to helping customers streamline therapeutic development, minimize risk, and ease the burden on their quality systems. Learn more here.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
We offer the Residual TrypLE ELISA Kit (Cat. No. A6533101) that can specifically detect the recombinant protease (rProtease) in TrypLE reagent.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
There is no upper limit for TrypLE enzyme incubation. You can incubate your cells with TrypLE longer to detach cells. 30-45 min at 37 degrees C will be usually fine. Or you can consider using TrypLE Select Enzyme (10X) (Cat. No. A1217701 or A1217702 or A1217703) or CTS TrypLE Select Enzyme (Cat. No. A4738001) depending upon the application.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Our TrypLE enzymes are very gentle, and there is no need to use serum or individual proteins to inactivate the enzyme. The TrypLE enzyme activity is inactivated by dilution using the following procedure:
After cell dissociation, transfer the cell suspension to a 15-mL conical tube. Centrifuge at 100 x g for 5-10 min to remove residual enzyme activity. Discard the supernatant and resuspend the cell pellet in 2-5 mL of pre-warmed complete medium.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
All the above products contain rProtease, a non-animal, trypsin-like enzyme formulation used for the dissociation of attachment-dependent cell lines. This alternative for porcine trypsin is a recombinant enzyme derived from microbial fermentation.
The TrpLE Express, TrypLE Select, and CTS TrypLE Select products differ in their production and testing methods and in the documentation provided.
TrypLE Select is for the industrial market and has release-testing both for endotoxins and enzyme activity. This material also has a Drug Master File on record. It is manufactured in AOF-dedicated equipment and is intended for Research Use/Further Cell Culture Manufacturing.
TrypLE Express is manufactured without use of dedicated equipment and is intended for Research Use Only. Not for use in diagnostic procedures.
CTS TrypLE Select is manufactured under scalable cGMP conditions using dedicated equipment and is designed for clinical applications. This product has a Drug Master File on record and is intended for Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. Not intended for direct administration into humans or animals.
To read more about TrypLE and cell release times using this reagent, please go here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin/tryple-express.html#1).
Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.