Search Thermo Fisher Scientific
Certificates
SDS
Citations & References (1)
Invitrogen™
Novex™ Tris-Glycine SDS Sample Buffer (2X)
Novex Tris-Glycine SDS Sample Buffer (2X) is used to prepare protein samples for denaturing gel electrophoresis using Tris-Glycine gels. ItRead more
PromotionPromo code:P5906365
Don't miss out: buy 3, only pay for 2
PromotionPromo code:RPUZZ25
Stock up on essentials to piece your discovery together
| Catalog Number | Quantity |
|---|---|
| LC2676 | 20 mL |
Catalog number LC2676
Price (CNY)
353.00
Each
In stock
Quantity:
20 mL
Price (CNY)
353.00
Each
Novex Tris-Glycine SDS Sample Buffer (2X) is used to prepare protein samples for denaturing gel electrophoresis using Tris-Glycine gels. It has a pH of 6.8 and contains bromophenol blue as a tracking dye.
See all available buffers and reagents available for SDS-PAGE
To use: Heat the sample in a 1X dilution (reduced or non-reduced) at 85°C for 2–5 minutes for optimal results. Heating samples at 100°C in SDS-containing buffers results in proteolysis.
See all available buffers and reagents available for SDS-PAGE
To use: Heat the sample in a 1X dilution (reduced or non-reduced) at 85°C for 2–5 minutes for optimal results. Heating samples at 100°C in SDS-containing buffers results in proteolysis.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical Name or MaterialSample Loading Buffer
Recommended StorageTris-Glycine Sample Buffer (2X) containing sodium dodecyl sulfate (SDS) at pH 6.8 with bromophenol blue.
Store at 2°C to 8°C.
Store at 2°C to 8°C.
Concentration2X
Physical FormLiquid
Product LineNovex
Quantity20 mL
Unit SizeEach
Have questions about this product? Ask our AI assisted search.
Generating response
This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our
Privacy Notice.
Customers who viewed this item also viewed
Documents & Downloads
Certificates
Search by lot number or partial lot number
Lot #Certificate TypeDateCatalog Number(s)
3158080Certificate of AnalysisApr 02, 2025LC2676
3156377Certificate of AnalysisMar 31, 2025LC2676
3001366Certificate of AnalysisOct 28, 2024LC2676
2896349Certificate of AnalysisMay 31, 2024LC2676
2844065Certificate of AnalysisFeb 10, 2024LC2676
5 results displayed, search above for a specific certificate
Safety Data Sheets
SDSFrequently asked questions (FAQs)
The formulations of buffers for our precast protein gels can be found at this link: https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-electrophoresis-buffers-reagents.html
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The Laemmli buffer or 2X SDS Buffer is composed of the following: 100 mM Tris HCl , pH 6.8, 200 mM dithiothreitol, 4% SDS, 0.2% bromophenol blue, 20% glycerol. 2X SDS gel loading buffer lacking dithiothreitol can be stored at room temperature. Dithiothreitol should then be added, just before use.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
If the Tricine gel is run with Tris-Glycine sample buffer, the bands will behave abnormally and resolve poorly. If the Tricine gel is accidentally run with Tris-Glycine running buffer, the gel will take longer to run and the resolution, especially for smaller proteins, will be worse than when the proteins are run on a Tris-Glycine gel with Tris-Glycine buffers. This is due to a combination of increase in stack area size (glycine is a slower ion than Tricine) and the higher ionic strength of the Tricine gel.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
SDS in a 4X sample buffer concentrate tends to precipitate from solution and to make the solution viscous and difficult to pipette. The LDS is much more soluble.
Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.
No, CTAB will not work with any of our gels except for the NuPAGE Tris-Acetate gels. To use CTAB, you would need to use a running buffer of 50 mM acetic acid and 50 mM beta-alanine in equal concentrations. You would also need to switch the electrodes. Since CTAB is a cationic detergent, this would establish conditions for running a basic protein towards the anode (into the gel).
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Citations & References (1)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Identification of components of protein complexes using a fluorescent photo-cross-linker and mass spectrometry.
Journal:Anal Chem
PubMed ID:12033289
'This study describes a novel method for improving the specific recognition, detection, and identification of proteins involved in multiprotein complexes. The method is based on a combination of coimmunoprecipitation, chemical cross-linking, and specific fluorescent tagging of protein components in close association with one another. Specific fluorescent tagging of the protein
1 total citations