Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Katalognummer | Beschreibung |
---|---|
A41195 | Includes 5 x MicroAmp TriFlex 3 x 32-well PCR Reaction Plates (100 plates total) |
A41194 | Includes MicroAmp 8-Tube Strips with Attached Domed Caps, 0.2 mL (125 strips) |
This MiniAmp Thermal Cycler kit includes a thermal cycler plus either MicroAmp 8-Tube Strips with Attached Domed Caps or MicroAmp TriFlex PCR Reaction Plates with MicroAmp 32-Well Clear Adhesive Films.
MiniAmp Thermal Cycler features
MicroAmp 8-Tube Strips with Attached Domed Caps
MicroAmp 8-Tube Strips with Attached Domed Caps feature individually attached domed caps, dual large end-tabs, and graduated 20-μL markers on each tube to help prevent cross-contamination, pipetting errors, and sample identification errors.
MicroAmp TriFlex 3 x 32-Well PCR Reaction Plates
MicroAmp TriFlex 3 x 32-Well PCR Reaction Plates have a unique design with three identical polypropylene 32-well PCR plates connected by dual side-tabs for use with 32-well and standard 96-well thermal blocks. These high-quality plates offer thin-wall construction for excellent heat transfer from thermal block to sample and are validated for use with Applied Biosystems PCR thermal cyclers for optimal fit and performance.
MicroAmp 32-Well Clear Adhesive Films
MicroAmp 32-Well Clear Adhesive Film is designed specifically for use with MicroAmp TriFlex 3 x 32-Well PCR Reaction Plates. The film provides an alternative to cap strips and seals 32-well samples securely to prevent PCR sample evaporation and well-to-well contamination.
There is no universal ramp rate for a thermal cycler because both the block and sample ramp rates are different for each thermal cycler. The sample ramp rate changes with the volume of the sample since more time and energy are required to heat higher sample volumes. The ramp rate may also need to be changed for a particular PCR assay or primer pair if you are trying to use the same PCR assay on two different thermal cyclers, for example.
As a reference, the block ramp rate commonly used with DreamTaq Polymerase on the MiniAmp thermal cycler is 3.0 C/sec. In a 50 µL PCR reaction, this translates to a sample ramp rate of ~1 C/sec.
Please refer to the following application note for further information about thermocycler ramp rates: https://assets.thermofisher.com/TFS-Assets/LSG/brochures/Thermal_Cycler_Ramp_RatesAppNote.pdf
Find additional tips, troubleshooting help, and resources within ourPCR Thermal Cyclers Support Center.
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