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Zitierungen und Referenzen (17)
Invitrogen™
FAST DiO™ Solid; DiOΔ9,12-C18(3), ClO4 (3,3'-Dilinoleyloxacarbocyanine Perchlorate)
FAST DiO ist ein ungesättigtes Analogon von DiO (DiOC18(3)), das Berichten zufolge ∼50 % schneller migriert. Es ist schwach fluoreszierendWeitere Informationen
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| Katalognummer | Menge |
|---|---|
| D3898 | 5 mg |
Katalognummer D3898
Preis (EUR)
664,00
Each
Geschätzte Verfügbarkeit Ausstehend
Menge:
5 mg
Preis (EUR)
664,00
Each
FAST DiO ist ein ungesättigtes Analogon von DiO (DiOC18(3)), das Berichten zufolge ∼50 % schneller migriert. Es ist schwach fluoreszierend in Wasser, aber sehr fluoreszierend und ziemlich photostabil, wenn es in Membranen integriert wird. Es hat einen extrem hohen Extinktionskoeffizienten und der angeregte Zustand in Lipidumgebungen dauert nur kurz an (∼1 Nanosekunde). Nach dem Auftragen auf Zellen diffundiert der Farbstoff seitlich innerhalb der Plasmamembran.
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
FarbeGrün, Grün
NachweisverfahrenFluoreszent, Fluoreszent
Zur Verwendung mit (Geräte)Fluoreszenzmikroskop, Fluoreszenzmikroskop
ProduktlinieFAST DiO™
Menge5 mg
VersandbedingungRaumtemperatur, Raumtemperatur
MarkertypFluorescent Dye
ProdukttypDiOΔ9 12-C18(3) ClO4
SubCellular LocalizationZellmembranen & Lipide, Lipids
Unit SizeEach
Inhalt und Lagerung
Bei -5 bis -30 °C lagern und vor Licht schützen.
Have questions about this product? Ask our AI assisted search.
I stained my cells with a lipophilic cyanine dye, like DiI, but the signal was lost when I tried to follow up with antibody labeling. Why?
How long does it take for lipophlic tracers to transport along the membrane? How much faster are the FAST lipophilic dyes?
I want to label two cell populations and then perform a cell fusion assay. Which reagents are best for imaging this?
Which form of the lipophilic tracers (DiO, DiI, DiD, etc) should I use?
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Zertifikate
Nach Chargennummer oder Teil-Chargennummer suchen
Chargen-Nr.Certificate TypeDateCatalog Number(s)
2738277Certificate of Analysis16. Nov. 2023D3898
2351950Certificate of Analysis23. Juni 2021D3898
2298130Certificate of Analysis15. Feb. 2021D3898
2181601Certificate of Analysis11. März 2020D3898
2157203Certificate of Analysis04. Okt. 2019D3898
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Sicherheitsdatenblätter
SDBProduct Information
Handbuch Molecular Probes®
Häufig gestellte Fragen (FAQ)
Since these dyes insert into lipid membranes, any disruption of the membranes leads to loss of the dye. This includes permeabilization with detergents like Triton X-100 or organic solvents like methanol. Permeabilization is necessary for intracellular antibody labeling, leading to loss of the dye. Instead, a reactive dye such as CFDA SE should be used to allow for covalent attachment to cellular components, thus providing for better retention upon fixation and permeabilization.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
The transport is fairly slow, around 6 mm/day in live tissue and slower in fixed tissue, so diffusion of lipophilic carbocyanine tracers from the point of their application to the terminus of a neuron can take several days to weeks The FAST DiO and DiI analogs (which have unsaturated alkyl tails) can improve transport rate by around 50%.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Select the dye that is compatible with your available excitation source(s) and emission filter set/channels. The solid, paste and crystal forms can be applied directly to neurons in tissues. For labeling cells in culture or microinjection, the lipophilic dyes in solution or solid form can be used.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Lipophilic cyanine dyes are preferred for this sort of assay, since they insert into cellular membranes and then, upon fusion, are shared by the fused cells as the membranes are shared. For example, one cell population can be labeled with DiI (orange-red) and another cell population can be labeled with DiO (green), and when the cells fuse, the combined color appears yellow (when imaged with a dual-bandpass filter set).
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Zitierungen und Referenzen (17)
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Zitierungen und Referenzen
Abstract
Lipid rafts in the maintenance of synapses, dendritic spines, and surface AMPA receptor stability.
Journal:J Neurosci
PubMed ID:12716933
'Cholesterol/sphingolipid microdomains (lipid rafts) in the membrane are involved in protein trafficking, formation of signaling complexes, and regulation of actin cytoskeleton. Here, we show that lipid rafts exist abundantly in dendrites of cultured hippocampal neurons, in which they are associated with several postsynaptic proteins including surface AMPA receptors. Depletion of
Fluorescence methods to detect phase boundaries in lipid bilayer mixtures.
Journal:Biochim Biophys Acta
PubMed ID:15992943
'Phase diagrams of lipid mixtures can show several different regions of phase coexistence, which include liquid-disordered, liquid-ordered, and gel phases. Some phase regions are small, and some have sharp boundaries. The identity of the phases, their location in composition space, and the nature of the transitions between the phases are
Carbocyanine dyes with long alkyl side-chains: broad spectrum inhibitors of mitochondrial electron transport chain activity.
Journal:Biochem Pharmacol
PubMed ID:7763312
'Certain indocarbocyanine, thiacarbocyanine, and oxacarbocyanine dyes possessing short alkyl side-chains (one to five carbons) are potent inhibitors of mammalian mitochondrial NADH-ubiquinone reductase (EC 1.6.99.3) activity (Anderson et al., Biochem Pharmacol 41: 677-684, 1991; Anderson et al., Biochem Pharmacol 45: 691-696, 1993; Anderson et al., Biochem Pharmacol 45: 2115-2122, 1993), and
Evidence for a role of dendritic filopodia in synaptogenesis and spine formation.
Journal:Neuron
PubMed ID:8755481
Axo-dendritic synaptogenesis was examined in live hippocampal cell cultures using the fluorescent dyes DiO to label dendrites and FM 4-64 to label functional presynaptic boutons. As the first functional synaptic boutons appeared in these cultures, numerous filopodia (up to 10 micron long) were observed to extend transiently (mean lifetime 9.5
A map of pheromone receptor activation in the mammalian brain.
Journal:Cell
PubMed ID:10219242
In mammals, the detection of pheromones is mediated by the vomeronasal system. We have employed gene targeting to visualize the pattern of projections of axons from vomeronasal sensory neurons in the accessory olfactory bulb. Neurons expressing a specific receptor project to multiple glomeruli that reside within spatially restricted domains. The
17 total citations