DMEM/F-12, HEPES
DMEM/F-12, HEPES
Gibco™

DMEM/F-12, HEPES

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth ofRead more
Have Questions?
Change viewbuttonViewtableView
Catalog NumberQuantity
31330095
also known as 31330-095
10 x 500 mL
11330032Promo Image500 mL
31330038
also known as 31330-038
500 mL
11330057Promo Image10 x 500 mL
11330107Promo Image10 L
Catalog number 31330095
also known as 31330-095
Price (EUR)
243,65
Online Exclusive
277,00
Save 33,35 (12%)
Each
Estimated availability date  Pending  
Add to cart
Quantity:
10 x 500 mL
Customize this product
Price (EUR)
243,65
Online Exclusive
277,00
Save 33,35 (12%)
Each
Add to cart
Ask our AI about this Product

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of Gibco™ DMEM / F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM / F-12 is modified as follows:

With: L-glutamine, HEPES, Phenol Red

The complete formulation is available.Gibco™ DMEM / F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components.

DMEM / F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM / F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM / F-12 uses a sodium bicarbonate buffer system and therefore requires a 5-10% CO2 environment to maintain physiological pH.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product TypeDMEM (Dulbecco's Modified Eagle Medium)/F-12
Quantity10 x 500 mL
Shelf Life12 Months
ClassificationAnimal Origin-free
Culture TypeMammalian Cell Culture
FormLiquid
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesGlutamine, HEPES, Phenol Red, High Glucose, Sodium Pyruvate
Unit SizeEach
Have questions about this product? Ask our AI assisted search.
This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our Privacy Notice.

Frequently asked questions (FAQs)

The lids of DMEM/F-12, HEPES medium (Cat. No. 31330038, 11330032, 31330095, 11330057, 11330107) have a different color now. Have there been any changes in the composition of the medium?

The color change of the lid has no effect on the sterility or functionality of the product. There has been no change to the end product DMEM/F-12, HEPES medium (Cat. No. 31330038, 11330032, 31330095, 11330057, 11330107); the medium still has the same composition.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer DMEM/F12 medium without folic acid?

We do not offer DMEM/F12 medium without folic acid as a standard catalog product. However, our Customs group can make it for you as a custom media. If you are interested, please complete this Custom order inquiry form PDF (https://www.thermofisher.com/content/dam/LifeTech/global/applied-sciences/pdfs/Bioproduction/Gibco-Custom-Media-Buffers-Request-Form.pdf) and email it to custommedia@thermofisher.com. Our Customs group will review your requirements and get back to you within a couple of days with pricing, ordering information, and lead time. After that, you have the choice of proceeding with an “official” order or declining.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Customers who viewed this item also viewed



Documents & Downloads

Certificates

Lot #Certificate TypeDateCatalog Number(s)
3166061Certificate of AnalysisJul 13, 202511330099, 11330032, 11330107
3157151Certificate of AnalysisJul 09, 202511330099, 11330032, 11330107
3203873Certificate of AnalysisJul 09, 202511330099, 11330032, 11330107
3157153Certificate of AnalysisJun 21, 202511330099, 11330032, 11330107
3013580Certificate of AnalysisJun 19, 202511330099, 11330032, 11330107
5 results displayed, search above for a specific certificate

Safety Data Sheets

Citations & References (6)

Citations & References
Abstract
Patient-derived glioblastoma organoids as real-time avatars for assessing responses to clinical CAR-T cell therapy
Authors:Meghan Logun, Xin Wang, Yusha Sun, Stephen J Bagley, Nannan Li, Arati Desai, Daniel Y Zhang, MacLean P Nasrallah, Emily Ling-Lin Pai, Bike Su Oner, Gabriela Plesa, Donald Siegel, Zev A Binder, Guo-Li Ming, Hongjun Song, Donald M O'Rourke
Journal:Cell Stem Cell
PubMed ID:39657679
Patient-derived tumor organoids have been leveraged for disease modeling and preclinical studies but rarely applied in real time to aid with interpretation of patient treatment responses in clinics. We recently demonstrated early efficacy signals in a first-in-human, phase 1 study of dual-targeting chimeric antigen receptor (CAR)-T cells (EGFR-IL13Rα2 CAR-T cells) ... More
Tissue-specific expression of the nonneuronal promoter of the aromatic L-amino acid decarboxylase gene is regulated by hepatocyte nuclear factor 1.
Authors: Aguanno A; Afar R; Albert V R;
Journal:J Biol Chem
PubMed ID:8626808
'The rat aromatic l-amino acid decarboxylase (AADC) gene contains alternative promoters which direct expression of neuronal and nonneuronal mRNAs that differ only in their 5''-untranslated regions (UTRs). We have analyzed the expression of the nonneuronal promoter of the rat AADC gene in the kidney epithelial cell line LLC-PK1 and in ... More
Fabrication of synthetic polymer coatings and their use in feeder-free culture of human embryonic stem cells.
Authors:Nandivada H, Villa-Diaz LG, O'Shea KS, Smith GD, Krebsbach PH, Lahann J,
Journal:Nat Protoc
PubMed ID:21720316
The culture of human embryonic stem (hES) cells in defined and xenogeneic-free conditions will contribute substantially to future biotechnological and medical applications. To achieve this goal, we developed the first fully defined synthetic polymer coating poly[2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium hydroxide] (PMEDSAH) that sustains long-term growth of hES cells in different culture media. ... More
Insulin and insulin-like growth factor I receptors utilize different G protein signaling components.
Authors:Dalle S, Ricketts W, Imamura T, Vollenweider P, Olefsky JM,
Journal:J Biol Chem
PubMed ID:11278773
We examined the role of heterotrimeric G protein signaling components in insulin and insulin-like growth factor I (IGF-I) action. In HIRcB cells and in 3T3L1 adipocytes, treatment with the Galpha(i) inhibitor (pertussis toxin) or microinjection of the Gbetagamma inhibitor (glutathione S-transferase-betaARK) inhibited IGF-I and lysophosphatidic acid-stimulated mitogenesis but had no ... More
Polycystin-1, the product of the polycystic kidney disease 1 gene, co-localizes with desmosomes in MDCK cells.
Authors:Scheffers MS, van der Bent P, Prins F, Spruit L, Breuning MH, Litvinov SV, de Heer E, Peters DJ,
Journal:Hum Mol Genet
PubMed ID:11063733
Polycystin-1 is a novel protein predicted to be a large membrane-spanning glycoprotein with an extracellular N-terminus and an intracellular C-terminus, harboring several structural motifs. To study the subcellular localization, antibodies raised against various domains of polycystin-1 and against specific adhesion complex proteins were used for two-color immunofluorescence staining. In Madine ... More
6 total citations

Other products to consider



Share catalog number, name or link

1x1 image pixel for data collection