SuperScript™ II Reverse Transcriptase
SuperScript™ II Reverse Transcriptase
Invitrogen™

SuperScript™ II Reverse Transcriptase

Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increasedRead more
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Catalog NumberNo. of Reactions
1806401450 Reactions
1806402210 Reactions
18064071200 Reactions
Catalog number 18064014
Price (EUR)/ Each
376,00
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Add to cart
No. of Reactions:
50 Reactions
Request bulk or custom format
Price (EUR)/ Each
376,00
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SuperScript™ II Reverse Transcriptase
Catalog number18064014
Price (EUR)/ Each
376,00
-
Add to cart

Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT. Mutations in the RNase H domain of the enzyme eliminate degradation of the RNA during first-strand cDNA synthesis, which results in higher yields of full-length cDNA.

SuperScript reverse transcriptases are the most highly trusted and widely used RTs with over 50,000 citations, reviews, and publications to date. The enzyme is purified to near homogeneity from E. coli containing the modified pol gene of Moloney Murine Leukemia Virus.

Features of SuperScript II Reverse Transcriptase include:
• High cDNA yields and more full-length product
• Generation of cDNA up to 12.3 kb
• First-strand cDNA synthesis at higher temperatures than conventional MMLV RT

Note: The latest member of the SuperScript RT family, SuperScript IV Reverse Transcriptase, features enhanced thermostability, processivity, yields, and performance with any RNA samples, including those of suboptimal purity or integrity.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatStand-alone Enzyme
GC-Rich PCR PerformanceHigh
Reaction Speed50 min.
TechniqueReverse Transcription
Optimal Reaction Temperature42°C
Quantity10,000 Units
Reverse TranscriptaseSuperScript II
Ribonuclease H ActivityReduced
Shipping ConditionWet Ice
Concentration200 U/μL
Final Product TypeFirst-Strand cDNA
No. of Reactions50 Reactions
Reaction FormatSeparate components
Reagent TypeReverse Transcription
Size (Final Product)Up to 12.3 kb
Starting MaterialRNA
Unit SizeEach
Contents & Storage

• SuperScript II Reverse Transcriptase, 50 μL (10,000 units at 200 U/μL)
• 5X First-strand buffer
• 100 mM DTT

Store at –20°C.