Fetal Bovine Serum, qualified, United States

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*** NEW CATALOG NUMBER***

These products are being replaced with Premium (FBS) performance-based products. The Premium product specifications are improved and product stability, performance, manufacturing, storage, and transport processes are unchanged.

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Fetal Bovine Serum, qualified, United States

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Citations & References (33)

Gibco™

Fetal Bovine Serum, qualified, United States

Name: Fetal Bovine Serum, qualified, United States
SKU: 26140079

Important Update: We've recently introduced Premium Fetal Bovine Serum (FBS) specification-based products. These improved products are alternatives to our Qualified US origin SKUs. Product stability, performance, manufacturing, storage, and transport processes are unchanged.

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Catalog Number	Quantity	Format
26140079	500 mL	Bottle
A3160501	50 mL	One Shot
A3160502	10 x 50 mL	One Shot

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Our improved Premium Fetal Bovine Serum (FBS) features enhanced specifications with unchanged stability, manufacturing, storage, and transport processes.

Fetal Bovine Serum (FBS), Premium, bottle, 500 mL

Catalog number: A5670701

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Catalog number 26140079

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Gibco fetal bovine sera offer excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirements

Gibco Sera: Trusted Quality for Consistent Performance

Gibco Sera Category: Value Plus (Performance)

Use for general cell culture with common cell lines
Low endotoxin and hemoglobin levels
Endotoxin level: <10 EU/mL
Hemoglobin level: <25 mg/dL
Origin: United States

Gibco Serum Delivers

ISO 13485 certified, processed in FDA registered facilities
Triple filtered at 0.1 μm
Gibco bottle is easier to use in the hood, reduces the risk of contamination and helps you perform cell culture more consistently
Peel-off sticker on label provides handy reference for lot number and expiration date recording in lab notebook

For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.

Specifications

Endotoxin Concentration≤10 EU/mL

Hemoglobin Concentration≤25 mg/dL

Purity or Quality GradeQualified

Shipping ConditionFrozen

SpeciesCattle/Bovine

AgeFetal

Country of OriginUnited States

FormLiquid

FormatBottle

Product TypeFetal Bovine Serum

Quantity500 mL

Serum TreatmentStandard (Sterile-filtered)

SterilitySterile

Sterilization MethodTriple-filtered, 0.1 μm

Unit SizeEach

Contents & Storage

Storage conditions: ≤-10°C
Shipping conditions: Frozen

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Frequently asked questions (FAQs)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol¹.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How long can I store Gibco Fetal Bovine Serum at 4 degrees C?

This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I thaw Gibco Fetal Bovine Serum?

We recommend thawing the serum overnight at 4 degrees C or in a 37 degrees C water bath, removing as soon as it is thawed. Once thawed, aliquot into single-use sizes and freeze the aliquots. Each aliquot should ideally be thawed only one additional time as repeated freeze-thaw cycles are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I minimize lot-to-lot variability in serum?

You can test each new lot to be sure that it works for your specific applications. Alternatively, our serum sales team can determine the most consistent, highest-performing serum lot available using the Gibco iMatch Sera Lot Matching Tool.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I aliquot and refreeze serum?

Yes, after the overnight thaw, you can aliquot the serum into smaller, single-use aliquots and refreeze it.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Fetal Bovine Serum, qualified, United States FAQs

Figures

Consistency of HEK293 Cell Growth in Gibco® FBS:

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Documents & Downloads

Certificates

Search by lot number or partial lot number

Lot #Certificate TypeDateCatalog Number(s)

U3241226RPCertificate of AnalysisJun 21, 202526140079, 26140087, 26140095

U3126623PCertificate of AnalysisMar 30, 202526140079, 26140087, 26140095

U3129988PCertificate of AnalysisFeb 20, 202526140079, 26140087, 26140095

U3129988PCertificate of AnalysisFeb 20, 2025A3160502, A3160501

U3116590PCertificate of AnalysisFeb 14, 2025A3160502, A3160501

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Safety Data Sheets

SDS

Scientific Resources

Brochure: Gibco Sera Guide

Brochure: Gibco Cell Culture Sera for Bioprocessing

Brochure: One Shot FBS 50 mL Bottle

Application Note: Performance comparison of maxi-scale plasmid DNA isolation kits

Flyer: Gibco FBS FAQs (frequently asked questions)

Technical Note: Bone Metastasis Models using Cell Culture inserts

Green Features Fact Sheet: One Shot FBS 50 mL bottles

Citations & References (33)

Search citations by name, author, journal title or abstract text

Citations & References

Abstract

A functional role for the B56 alpha-subunit of protein phosphatase 2A in ceramide-mediated regulation of Bcl2 phosphorylation status and function.

Authors: Ruvolo Peter P; Clark Warren; Mumby Marc; Gao Fengqin; May W Stratford;

Journal:J Biol Chem

PubMed ID:11929874

'Recently it has been shown that the potent apoptotic agent ceramide activates a mitochondrial protein phosphatase 2A (PP2A) and promotes dephosphorylation of the anti-apoptotic molecule Bcl2 (Ruvolo, P. P., Deng, X., Ito, T., Carr, B. K., and May, W. S. (1999) J. Biol. Chem. 274, 20296-20300). In cells expressing Bcl2, ... More

Metabolism of 4 beta -hydroxycholesterol in humans.

Authors: Bodin Karl; Andersson Ulla; Rystedt Eva; Ellis Ewa; Norlin Maria; Pikuleva Irina; Eggertsen GÃ¶sta; BjÃ¶rkhem Ingemar; Diczfalusy Ulf;

Journal:J Biol Chem

PubMed ID:12077124

'One of the major oxysterols in the human circulation is 4 beta-hydroxycholesterol formed from cholesterol by the drug-metabolizing enzyme cytochrome P450 3A4. Deuterium-labeled 4 beta-hydroxycholesterol was injected into two healthy volunteers, and the apparent half-life was found to be 64 and 60 h, respectively. We have determined earlier the half-lives ... More

Activation of retinoic acid receptor-dependent transcription by all-trans-retinoic acid metabolites and isomers.

Authors: Idres Nadia; Marill Julie; Flexor Maria A; Chabot Guy G;

Journal:J Biol Chem

PubMed ID:12070176

'We have shown that four metabolites of all-trans-retinoic acid (ATRA) (4-oxo-, 4-OH-, 18-OH-, and 5,6-epoxy-RA) can induce maturation of NB4 promyelocytic leukemia cells (Idres, N., Benoit, G., Flexor, M. A., Lanotte, M., and Chabot, G. G. (2001) Cancer Res. 61, 700-705). To better understand the mechanism of action of ATRA ... More

Interaction of human breast fibroblasts with collagen I increases secretion of procathepsin B.

Authors: Koblinski Jennifer E; Dosescu Julie; Sameni Mansoureh; Moin Kamiar; Clark Katherine; Sloane Bonnie F;

Journal:J Biol Chem

PubMed ID:12072442

'Interactions of stromal and tumor cells with the extracellular matrix may regulate expression of proteases including the lysosomal proteases cathepsins B and D. In the present study, we determined whether the expression of these two proteases in human breast fibroblasts was modulated by interactions with the extracellular matrix component, collagen ... More

A ligand-inducible epidermal growth factor receptor/anaplastic lymphoma kinase chimera promotes mitogenesis and transforming properties in 3T3 cells.

Authors: Piccinini Gina; Bacchiocchi Roberta; Serresi Michela; Vivani Caterina; Rossetti Silvia; Gennaretti Claudia; Carbonari Damiano; Fazioli Francesca;

Journal:J Biol Chem

PubMed ID:11919185

'Oncogenic rearrangements of the anaplastic lymphoma kinase (ALK) gene, encoding a receptor type tyrosine kinase, are frequently associated with anaplastic large cell lymphomas. Such rearrangements juxtapose the intracellular domain of ALK to 5''-end sequences belonging to different genes and create transforming fusion proteins. To understand how the oncogenic versions of ... More

33 total citations