Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
產品號碼 | Quantity |
---|---|
15250061 | 100 mL |
There are a number of reagents used to quench fluorescence on the outside of live cells or in the media (background fluorescence). Our Backdrop Background Suppressor reagents are a great option to quench blue, green, and red fluorescent dyes, various media components and fluorescent proteins. Another commonly used reagent is the cell impermeant colorimetric dye, Trypan blue.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Trypan Blue is filter sterilized through a 0.22 µm filter.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
It is a 0.85% saline solution.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Unfortunately, there is no definitive answer to this question. The rate at which the cell-impermeant dye is absorbed depends on the cell type, their state of health, nourishment, engulfment activity, etc.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Exposure to light may degrade the dye and these contaminants may promote precipitation. Trypan blue can also form orange/red fibrous aggregates if exposed to refrigeration or freezing temperatures.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
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