Human Dermal Fibroblasts, neonatal (HDFn)
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Human Dermal Fibroblasts, neonatal (HDFn)

Primary human dermal fibroblasts isolated from neonatal foreskin, cryopreserved at the end of the primary culture. • Viability at leastRead more
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Catalog NumberQuantity
C0045C1 vial
Catalog number C0045C
Price (TWD)
22,890.00
Online offer
Ends: 30-Sep-2025
32,700.00
Save 9,810.00 (30%)
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Quantity:
1 vial
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Price (TWD)
22,890.00
Online offer
Ends: 30-Sep-2025
32,700.00
Save 9,810.00 (30%)
Each
Add to cart
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Primary human dermal fibroblasts isolated from neonatal foreskin, cryopreserved at the end of the primary culture.
• Viability at least 70%
• ≥500,000 viable cells/vial
• Capable of at least 16 population doublings
• Mycoplasma: Not Detected
• Hepatitis B: Not Detected
• Hepatitis C: Not Detected
• HIV-1: Not Detected
• Bacteria, yeast, and other fungi: Not Detected
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Donor SourceSingle Donor
No. of Cells≥500000
Product LineCascade Biologics™
Product TypeHuman Dermal Fibroblasts, Neonatal
Quantity1 vial
Tested ForHepatitis B (HBV), Hepatitis C (HCV), Sterility Testing (Bacteria & Fungi), Mycoplasma, Growth Performance Assay, HIV-1
AgeNewborn (14 days or less)
Cell TypeFibroblast Cells (Dermal)
Culture TypeAdherent Cell Culture
FormCryopreserved
SpeciesHuman
Unit SizeEach
Contents & Storage
Liquid nitrogen temperature
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Frequently asked questions (FAQs)

Can I get passage information for Human Dermal Fibroblasts, adult (HDFa) (Cat. No. C0135C) and Human Dermal Fibroblasts, neonatal (HDFn) (Cat. No. C0045C)?

Human Dermal Fibroblasts, adult (HDFa) (Cat. No. C0135C) and Human Dermal Fibroblasts, neonatal (HDFn) (Cat. No. C0045C) are primary human dermal fibroblasts isolated from adult skin and neonatal foreskin, respectively. They are cryopreserved at the end of the primary culture stage and not passaged.

What are your Human Dermal Fibroblast (HDF) cells grown in?

HDF cells are grown in Medium 106 with the addition of Low Serum Growth Supplement.

What media do you use during the first passage of primary cells?

We use various media and supplements for the various primary cells. Details can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/primary-cell-culture.html).

How is the population doubling calculated for primary cells?

Cumulative population doublings are calculated from the seeding density and harvesting density from each culture level.

What are typical primary cell yields from tissue?

It varies a lot depending on the age of donor, size of tissues, locations, and conditions arrived to our facility. Sometime a small amount of tissue gives us many cells and sometimes a large amount of tissue gives us a very small lot. Typically, we can get 30-300 vials.

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Lot #Certificate TypeDateCatalog Number(s)
2456041Certificate of AnalysisJan 29, 2025C0045C
2505787Certificate of AnalysisJan 29, 2025C0045C
2582026Certificate of AnalysisJan 29, 2025C0045C
2584360Certificate of AnalysisJan 29, 2025C0045C
2551143Certificate of AnalysisJan 29, 2025C0045C
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