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CellTrace™ Far Red Cell Proliferation Kit, for flow cytometry
CellTrace™ Far Red Cell Proliferation Kit, for flow cytometry
Invitrogen™

CellTrace™ Far Red Cell Proliferation Kit, for flow cytometry

CellTrace™ Far Red Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multipleRead more
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Catalog NumberQuantity
C34564Promo Image180 Assays
C34572Promo Image20 Assays
Catalog number C34564
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CellTrace™ Far Red Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry.

• Superior performance—bright, single-peak staining enables visualization of multiple generations
• Long-term signal stability—well-retained in cells for several days post stain
• Versatile—multiple colors available to easily combine with antibodies or markers of cell function, such as GFP
• Simple, robust staining protocol

View a selection guide for all CellTrace™ Cell Proliferation Kits for flow cytometry.

Superior fluorescent staining
Successful proliferation analysis by dye dilution requires an extremely bright dye to distinguish fluorescently labeled cells from auto-fluorescence after several cell divisions. The intense fluorescent staining provided by CellTrace™ Far Red dye enables the visualization of six or more generations of proliferating cells before the signal is overwhelmed by intrinsic cellular auto-fluorescence. This kit enables consistent, homogeneous staining results with very little fluorescence variation between cells in a population, so distinct generations can be seen without any requirement for complex modeling software.

Long-term signal retention
Unlike stains that label the lipid membrane of cells, CellTrace™ Far Red stain easily crosses the plasma membrane and covalently binds inside cells where the stable, well-retained fluorescent dye offers a consistent signal, even after several days in a cell culture environment. CellTrace™ Far Red dye binds covalently to all free amines on the surface and inside of cells and shows little cytotoxicity, with minimal observed effect on the proliferative ability or biology of cells.

Easy multiplexing with other fluorophores
The red excitation at 630 nm and emission at 661 nm of CellTrace™ Far Red dye make it ideal for multiplexing due to the limited spectral overlap with other common dyes (Alexa Fluor™ 488, FITC, and RPE) and fluorescent proteins (Green Fluorescent Protein (GFP) and mCherry).

Simple, robust staining protocol
The CellTrace™ Far Red Cell Proliferation Kit contains convenient single-use vials of dry dye to permit small-scale experiments without preparing excess quantities of dye. A stock solution is prepared by dissolving the contents of a vial in anhydrous DMSO prior to use. To stain 1 mL of cells in protein-free medium, 1 μL of this stock solution is typically used. Cells should be stained for 20 minutes at room temperature with gentle agitation. A brief wash with complete medium will then quench any dye remaining in solution.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Dye TypeContains 9 vials of CellTrace™ Far Red (lyophilized powder) and 1 vial of DMSO (500μL)
FormLyophilized
FormatTube(s)
Quantity180 Assays
Shipping ConditionRoom Temperature
Emission633/635
For Use With (Application)Proliferation Assay
For Use With (Equipment)Flow Cytometer
Product LineCellTrace
Product TypeCell Proliferation Kit
Unit SizeEach
Contents & Storage
Continas 9 vials of CellTrace™ Far Red (lyophilized powder) and 1 vial of DMSO (500 μL). Store in freezer (-5 to -30°C).
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Fluorescence spectra

Fluorescence spectra

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Lot #Certificate TypeDateCatalog Number(s)
3143148Certificate of AnalysisJun 17, 2025C34564
3148261Certificate of AnalysisJun 17, 2025C34572
3143116Certificate of AnalysisApr 13, 2025C34564
2961100Certificate of AnalysisAug 05, 2024C34564
2929975Certificate of AnalysisJul 26, 2024C34572
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Safety Data Sheets

Frequently asked questions (FAQs)

The approximate excitation and emission peaks of this product after hydrolysis are 630 nm and 661 nm, respectively. Cells labeled with CellTrace Far Red reagent can be visualized by fluorescence microscopy using standard Cy 5 filter sets or analyzed by flow cytometry in an instrument equipped with a 633/635 nm excitation source and APC channel.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Calcein, AM and FDA (fluorescein diaceate) are examples of some dyes used for this application. Since these dyes are not incorporated or covalently attached to any cellular components, they may have a short retention time as some cell types may actively efflux the dye out of the cells. The CellTracker and CellTrace dyes include either a mild thiol-reactive chloromethyl group or amine-reactive succinnimidyl ester group to allow for covalent binding to cellular components, providing for better retention. As with any reagent, one should empirically determine retention times for the cell type used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Calcein, AM is a good choice for cell tracking and as a general cytoplasmic stain. However, it doesn't bind to anything and may be actively pumped out of the cells within a couple hours, which is likely what happened. The retention of Calcein within live cells is dependent upon the inherent properties of the cell type and culture conditions.

For long-term imaging, you may wish to consider a reactive cytoplasmic stains such as CFDA, SE or the CellTracker and CellTrace dyes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Yes, the CellTrace Far Red dye can be fixed with paraformaldehyde (PFA). The dye covalently binds to cells and will not wash out after permeabilization or fixation.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

We have not tested the use of the CellTrace reagents for co-culture applications. In theory, this may work, but you would have to test this on your cells of interest.

Find additional tips, troubleshooting help, and resources within our Cell Tracing and Tracking Support Center.

Citations & References (8)

Citations & References
Abstract
Appraising the suitability of succinimidyl and lipophilic fluorescent dyes to track proliferation in non-quiescent cells by dye dilution.
Authors:Filby A, Begum J, Jalal M, Day W,
Journal:
PubMed ID:25802116
Successful completion of the cell cycle usually results in two identical daughter progeny. This process of generational doubling is termed proliferation and when it occurs in a regulated fashion the benefits range from driving embryonic development to mounting a successful immune response. However when it occurs in a dis-regulated fashion, ... More
Microfluidic Encapsulation Supports Stem Cell Viability, Proliferation, and Neuronal Differentiation.
Authors:Hidalgo San Jose L, Stephens P, Song B, Barrow D,
Journal:Tissue Eng Part C Methods
PubMed ID:29258387
'Stem cell encapsulation technology demonstrates much promise for the replacement of damaged tissue in several diseases, including spinal cord injury (SCI). The use of biocompatible microcapsules permits the control of stem cell fate in situ to facilitate the replacement of damaged/lost tissue. In this work, a novel customized microfluidic device ... More
Isolation and Activation of Murine Lymphocytes.
Authors:Lim JF, Berger H, Su IH
Journal:J Vis Exp
PubMed ID:27842342
'B and T cells, with their extremely diverse antigen-receptor repertoires, have the ability to mount specific immune responses against almost any invading pathogen(1,2). Understandably, such intricate abilities are controlled by a large number of molecules involved in various cellular processes to ensure timely and spatially regulated immune responses(3). Here, we ... More
Coordinated Splicing of Regulatory Detained Introns within Oncogenic Transcripts Creates an Exploitable Vulnerability in Malignant Glioma.
Authors:Braun CJ, Stanciu M, Boutz PL, Patterson JC, Calligaris D, Higuchi F, Neupane R, Fenoglio S, Cahill DP, Wakimoto H, Agar NYR, Yaffe MB, Sharp PA, Hemann MT, Lees JA,
Journal:Cancer Cell
PubMed ID:28966034
Glioblastoma (GBM) is a devastating malignancy with few therapeutic options. We identify PRMT5 in an in vivo GBM shRNA screen and show that PRMT5 knockdown or inhibition potently suppresses in vivo GBM tumors, including patient-derived xenografts. Pathway analysis implicates splicing in cellular PRMT5 dependency, and we identify a biomarker that predicts sensitivity ... More
Synergistic activity of sorafenib and betulinic acid against clonogenic activity of non-small cell lung cancer cells.
Authors:Kutkowska J, Strzadala L, Rapak A,
Journal:Cancer Sci
PubMed ID:28846180
The highly selective multi-targeted agent sorafenib is an inhibitor of a number of intracellular signaling kinases with anti-proliferative, anti-angiogenic and pro-apoptotic effects in various types of tumors, including human non-small cell lung cancer (NSCLC). Betulin displays a broad spectrum of biological and pharmacological properties, including anticancer and chemopreventive activity. Combination ... More
8 total citations

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