sh-sy5y Cells | Thermo Fisher Scientific

Organism Type	Human	A subline of the neuroblastoma SK-N-SH line derived from a metastatic bone tumor. Grows as a mixture of floating and adherent cells.
Tissues	Brain
Phenotype	Either
Primary	no
Application	Cell Culture / Growth Conditions,Protein Expression,Stable Cell Transfection,Transient Transfection

Recommended Transfection Reagents

Plasmid DNA transfection and co-transfection: Lipofectamine® 3000 ( <30% transfection efficiency and 1 fold protein expression improvement over Lipofectamine® 2000)
RNAi transfection (siRNA/miRNA) and gene silencing: Lipofectamine® RNAiMAX

Products

Catalog #	Name	Size	Price (USD)	Qty
10569010	DMEM, high glucose, GlutaMAX™ Supplement, pyruvate, 500 mL	Each	42.65 Online Exclusive ~~48.40~~ Save 5.75 (12%)
11095080	MEM, 500 mL	Each	32.65 Online Exclusive ~~37.45~~ Save 4.80 (13%)
11765047	Ham's F-12 Nutrient Mix, 1000 mL	Each	56.65 Online Exclusive ~~65.00~~ Save 8.35 (13%)
11765054	Ham's F-12 Nutrient Mix, 500 mL	500 mL	36.65 Online Exclusive ~~41.77~~ Save 5.12 (12%)
11765062	Ham's F-12 Nutrient Mix, 10 x 500 mL	Each	312.65 Online Exclusive ~~360.00~~ Save 47.35 (13%)
11765070	Ham's F-12 Nutrient Mix, 6 x 1000 mL	Each	305.65 Online Exclusive ~~348.00~~ Save 42.35 (12%)
11875085	RPMI 1640 Medium, 1000 mL	Each	59.75
11875093	RPMI 1640 Medium, 500 mL	Each	37.37
11875101	RPMI 1640 Medium, 100 mL	Each	17.08
11875119	RPMI 1640 Medium, 10 x 500 mL	Each	322.00
11875127	RPMI 1640 Medium, 20 x 100 mL	Each	295.00
11875135	RPMI 1640 Medium, 6 x 1000 mL	Each	309.00
15290018	Amphotericin B, 20 mL	Each	32.65 Online Exclusive ~~37.62~~ Save 4.97 (13%)
17502048	N-2 Supplement (100X), 5 mL	Each	132.00
17504044	B-27™ Supplement (50X), serum free, 10 mL	Each	134.65 Online Exclusive ~~152.00~~ Save 17.35 (11%)
18292011	Lipofectin™ Transfection Reagent, 1 mL	Each	710.00
18324012	Lipofectamine™ Transfection Reagent, 1 mL	Each	682.00
21103049	Neurobasal™ Medium	Each	110.00
25030149	L-Glutamine (200 mM), 20 mL	Each	12.65 Online Exclusive ~~14.67~~ Save 2.02 (14%)
26140079	Fetal Bovine Serum, qualified, United States, 500 mL	Each	Request A Quote	-
K278001	Calcium Phosphate Transfection Kit	Each	734.00
V102020	pcDNA™4/TO Mammalian Expression Vector	20 µg	1,024.00
V102520	pcDNA™6/TR vector Mammalian Expression Vector	20 µg	886.00

Every step of the way, a wide range of cell health products

Maintaining healthy cells is the key to experimental success and reproducible research results. To give you confidence in the health of your cells every step of the way, we’ve highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. No matter how you are using your cells, you can count on these products to help keep them healthy.

GROW Gibco cell culture media & reagents Gibco growth factors Gibco FBS Countess II FL Automated Cell Counter		MODIFY Lipofectamine transfection reagents Neon electroporation transfection system Invitrogen RNAi products Recombinant proteins Dynabeads cell separation technology		DETECT Invitrogen imaging reagents Invitrogen flow cytometry reagents		ANALYZE EVOS cell imaging systems Attune NxT Flow Cytometer Qubit fluorometric quantification