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Gibco™
Amphotericin B
Amphotericin B is the generic version of Fungizone. 'Fungizone' is a trademark of E.R. Squibb & Sons, LLC. Amphotericin BRead more
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| Catalog Number | Quantity |
|---|---|
| 15290018 | 20 mL |
| 15290026 | 50 mL |
Catalog number 15290018
Price (USD)
34.65
Online Exclusive
37.62Save 2.97 (8%)
Each
-
Quantity:
20 mL
Price (USD)
34.65
Online Exclusive
37.62Save 2.97 (8%)
Each
Amphotericin B is the generic version of Fungizone. 'Fungizone' is a trademark of E.R. Squibb & Sons, LLC. Amphotericin B is an antifungal, produced by Streptomyces nodosus. It prevents the growth of fungi by causing an increase in fungal plasma membrane permeability. It actively binds to sterols and leads to the formation of pores. It is used to prevent the contamination of cell cultures by yeast and multicellular fungi. Gibco Amphotericin B contains 250 μg of amphotericin B and 205 μg of sodium deoxycholate per mL of distilled water. The recommended working concentration ranges from 0.25 to 2.50 μg/mL.
Dual-site cGMP manufacturing
For supply chain continuity, we manufacture Gibco Amphotericin B at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to the ISO 13485 standard, and are registered with the FDA as medical device manufacturers.
Dual-site cGMP manufacturing
For supply chain continuity, we manufacture Gibco Amphotericin B at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to the ISO 13485 standard, and are registered with the FDA as medical device manufacturers.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration0.25 to 2.5 μg/mL
For Use With (Application)Prevention of Cell Culture Contamination
Product LineFungizone™
Quantity20 mL
Shelf Life12 Months
Shipping ConditionDry Ice
FormLiquid
Product TypeAntifungal
SterilitySterile-filtered
Unit SizeEach
Contents & Storage
Storage conditions: -5°C to -20°C
Shipping conditions: Frozen on dry ice
Shelf life: 12 months from date of manufacture
Shipping conditions: Frozen on dry ice
Shelf life: 12 months from date of manufacture
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Lot #Certificate TypeDateCatalog Number(s)
3168690Certificate of AnalysisJun 08, 202515290026, 15290018
3203969Certificate of AnalysisJun 06, 202515290026, 15290018
3118244Certificate of AnalysisJun 04, 202515290026, 15290018
3150459Certificate of AnalysisMay 29, 202515290026, 15290018
3118240Certificate of AnalysisMay 22, 202515290026, 15290018
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Safety Data Sheets
SDSFrequently asked questions (FAQs)
Amphotericin B can be freeze-thawed 2-3 times without appreciable loss of potency.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Amphotericin B in solution is stable at 2-8 degrees C for approximately 4 weeks.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Amphotericin B is the generic version of Fungizone. Fungizone is a trademark of E.R. Squibb & Sons, LLC.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.
1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.
The following is a suggested procedure for determining toxicity levels and decontaminating cultures:
1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Citations & References (13)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Use of human tissue explants to study human infectious agents.
Journal:Nat Protoc
PubMed ID:19197269
The study of human cell-cell and cell-pathogen interactions that occur in the context of complex tissue cytoarchitecture is critical for deciphering the mechanisms of many normal and pathogenic processes. This protocol describes methods for culturing and infecting explants of human tissues to study the pathogenesis of human infectious agents and
Specific inhibition of ICAM-1 expression mediated by gene targeting with Triplex-forming oligonucleotides.
Journal:J Biol Chem
PubMed ID:12080053
Selected sequences in the DNA double helix can be specifically recognized by oligonucleotides via hydrogen bonding interactions. The resulting triple helix can modulate DNA metabolism and especially interfere with transcription in a gene-specific manner. To explore the potential of triplex-forming oligonucleotides (TFOs) as gene repressors, a TFO was designed to
Endogenous G Protein-coupled Receptor Kinase 6 Regulates M3 Muscarinic Acetylcholine Receptor Phosphorylation and Desensitization in Human SH-SY5Y Neuroblastoma Cells.
Journal:J Biol Chem
PubMed ID:11856737
We have previously shown that overexpression of G protein-coupled receptor kinase 6 (GRK6) enhanced the phosphorylation and desensitization of the endogenously expressed M(3) muscarinic acetylcholine (mACh) receptor in human SH-SY5Y neuroblastoma cells. In this study we have examined the potential role of endogenous GRK6 in the regulation of M(3) mACh
CD40-mediated Activation of NF-kappa B in Airway Epithelial Cells.
Journal:J Biol Chem
PubMed ID:12122011
We have reported previously that airway epithelial cells (AEC) express CD40 and that activation of this molecule stimulates the expression of inflammatory mediators, including the chemokine RANTES (regulated on activation normal T cell expressed and secreted). Because NF-kappaB regulates the expression of many inflammatory mediators, such as RANTES, we utilized
PC12 Cell Line: Cell Types, Coating of Culture Vessels, Differentiation and Other Culture Conditions.
Journal:Cells
PubMed ID:32295099
'The PC12 cell line is one of the most commonly used in neuroscience research, including studies on neurotoxicity, neuroprotection, neurosecretion, neuroinflammation, and synaptogenesis. Two types of this line are available in the ATCC collection: traditional PC12 cells grown in suspension and well-attached adherent phenotype. PC12 cells grown in suspension tend
13 total citations