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Compatible reagents
iBright Imaging Systems offer up to five imaging modes to support your applications. Efficiently and easily capture data from protein gels, nucleic acid gels, chemiluminescent western blots, fluorescent western blots, and more. Get the most from your investment by having the freedom to choose from multiple detection strategies to meet your experimental needs.
| Image mode | What kind of signal can be captured? |
Protein gel |
Colorimetric staining of gels (e.g., Coomassie, silver) and membranes (e.g., Ponceau S, Thermo Scientific Pierce Reversible Protein Stain), fluorescent staining of gels (e.g., Invitrogen SYPRO Ruby stain) |
Nucleic acid gel |
Ethidium bromide and a variety of fluorescent nucleic acid stains (e.g. Invitrogen SYBR stains) |
Chemiluminescent blot |
Chemiluminescence using all popular HRP and AP substrates (e.g., Thermo Scientific SuperSignal and Invitrogen WesternBreeze substrates) |
Fluorescent blot |
Fluorescence with popular RGB (visible range) and near-IR fluorophores (e.g., Invitrogen Alexa Fluor and Alexa Fluor Plus conjugates) |
Universal |
Custom mode to image samples containing multiple signals, such as chemiluminescence, fluorescence, colorimetric stains, and/or visible images; image display is similar to fluorescent blot mode and allows one to assign false color to any sample |
Fluorescent western blot imaging
With the five fluorescent channels of the iBright FL1500 Imaging System, one can image with many different fluorophore options. Up to 4-channel multiplexing is possible, utilizing fluorophores in both the visible (RGB) fluorescent range and the near-infrared (near-IR) range. The table below shows available fluorescence channels on iBright Imaging Systems and some examples of fluorophores that can be detected in each.
Fluorescence channels on the iBright FL1500 Imaging System
Excitation channel |
Filter range (nm) |
Emission channel |
Filter range (nm) |
EX1 |
455-485 |
EM1 |
508-557 |
EX2 |
515-545 |
EM2 |
568-617 |
EX3 |
608-632 |
EM3 |
675-720 |
EX4 |
610-660 |
EM4 |
710-730 |
EX5 |
745-765 |
EM5 |
800-850 |
Fluorescence channels on the iBright FL1500 Imaging System
Chemiluminescent western blot imaging
Examples of chemiluminescent reagents that can be detected using the iBright FL1500, iBright CL1500, and iBright CL750 Imaging Systems:
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FL1500 |
CL1500 |
CL750 |
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Protein gel imaging
Example protein gel stains that can be detected using the iBright FL1500, iBrightCL1500, and iBright CL750 Imaging Systems:
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FL1500 |
CL1500 |
CL750 |
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Deep purple stains |
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Oriole fluorescent stain |
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Flamingo fluorescent stain |
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Nucleic acid gel imaging
Examples of nucleic acid dyes and stains that can be detected using the iBright FL1500, iBright CL1500, and iBright CL750 Imaging Systems:
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FL1500 |
CL1500 |
CL750 |
Ethidium bromide |
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Hoechst blue |
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Colony counting
Examples of bacterial and mammalian cell colony labeling reagents that can be detected using the iBright FL1500, iBright CL1500, and iBright CL750 Imaging Systems:
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FL1500 |
CL1500 |
CL750 |
GFP (using transillumination) |
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GFP (using epi-illumination) |
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Crystal violet (using transillumination) |
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Crystal violet (using epi-illumination) |
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Blue/white visible (β-galactosidase/X-Gal/IPTG) |
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