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TrypLE™ Express Enzyme (1X), phenol red
TrypLE™ Express Enzyme (1X), phenol red
Actual product may vary
Gibco™

TrypLE™ Express Enzyme (1X), phenol red

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TrypLE Express is an animal origin-free, recombinant enzyme used for dissociating a wide range of adherent mammalian cells, including CHO,Read more
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Catalog NumberQuantity
12605093Promo Image5 L
12605010Promo Image100 mL
12605036Promo Image20 x 100mL
12605028Promo Image500 mL
Catalog number 12605093
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923.65
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TrypLE Express is an animal origin-free, recombinant enzyme used for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A549, primary human keratinocytes, and embryonic stem cells. TrypLE Express cleaves peptide bonds on the C-terminal sides of lysine and arginine, and is a direct replacement for trypsin. Its exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts.

We offer a variety of TrypLE formulations for a range of cell culture applications.

Compared to trypsin and other dissociation reagents, Gibco TrypLE Express is:

Gentle on cells

Room-temperature stable
Easy to use
Animal origin-free (AOF)

This TrypLE Express is modified as follows:

With

• Phenol Red

• EDTA

The formulation of TrypLE solutions is available, although we keep the enzyme concentration confidential.

Gentle On Cells
TrypLE Express' exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from enzymes present in some trypsin preparations and in other protease extracts.

Room-Temperature Stable
TrypLE Express is room-temperature stable and ready to use. TrypLE Express remains stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.

Easy to Use
TrypLE Express can be directly substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE Express, avoiding the need for trypsin inhibitors, such as FBS.

Animal Origin-Free (AOF)
Unlike porcine trypsin, TrypLE Express is free of animal-derived components.

cGMP Manufacturing and Quality System
For supply chain continuity, we manufacture Gibco TrypLE Express at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to the ISO 13485 standard, and are registered with the FDA as medical device manufacturers.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeMammalian Cells
ChelatorsEDTA
Concentration1 X
Culture TypeAdherent Cell Culture
Green FeaturesEnergy efficient, Sustainable packaging
OsmolalityCheck and Record
Product LineGibco™
Quantity5 L
RemarksRoom Temperature Stable
Shelf Life24 Months
Shipping ConditionRoom Temperature
SourceAnimal origin-free
Tested ForIn vitro Bioassay
ClassificationAnimal Origin-free
FormLiquid
Product TypeCell Culture Dissociation Reagent
SterilitySterile-filtered
With AdditivesPhenol Red, EDTA
Unit SizeEach
Contents & Storage
Storage conditions: 15°C to 30°C, protect from light
Alternate storage conditions: 2°C to 8°C or -5°C to -20°C
Shipping conditions: Ambient
Shelf life: 24 months from date of manufacture
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Lot #Certificate TypeDateCatalog Number(s)
3142768Certificate of AnalysisJun 25, 202512605010, 12605093, 12605028
3180905Certificate of AnalysisJun 25, 202512605010, 12605093, 12605028
3150523Certificate of AnalysisJun 15, 202512605010, 12605093, 12605028
3118112Certificate of AnalysisJun 15, 202512605010, 12605093, 12605028
3209703Certificate of AnalysisMay 29, 202512605010, 12605093, 12605028
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Safety Data Sheets

Limited Use Label Licenses (LULL)

'

Notice to Purchaser: The purchase of this product conveys to the purchaser the limited, non- transferable right to use the purchased amount of the product for mammailian and insect cell culture only, to (a) perform internal research for the sole benefit of the purchaser; (b) manufacture protein (or other biological material) for resale; and (c) perform research or manufacturing services by the purchaser on a fee for service or contract basis for or on behalf of third parties. However, the purchaser may transfer this product, its components, or materials made using this product to a third party (including contract research/manufacturing organizations), provided that each such third party agrees in writing to use such product, components, or materials solely on behalf of the purchaser, and such third party is restricted from further transferring any such product, components, or materials to any individual or entity other than the purchaser. No additional rights are granted. By purchasing this product, the purchaser agrees not to: (1) resell the product in any form; (2) use the product as a therapeutic agent or diagnostics test component (including, but not limited to, direct use for therapeutic purposes, use as an excipient in finished drug formulations, or use as an active pharmaceutical ingredient); (3) use the product in any applications for direct topical or in vivo use; (4) use the product in any applications for in vitro fertilization; (5) reverse engineer the product or cause the product to be reverse engineered; or (6) use the product for purposes other than what is indicated in this Limited Use Label License. The purchaser is responsible for obtaining all regulatory approvals necessary for any therapeutic or diagnostic use of the protein (or biological material) manufactured using this product. For information on obtaining additional rights, please contact outlicensing@thermofisher.com, Licensing and Commercial Supply, Thermo Fisher Scientific, 5823 Newton Drive, Carlsbad, CA, 92008, United States.

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Frequently asked questions (FAQs)

We offer the Residual TrypLE ELISA Kit (Cat. No. A6533101) that can specifically detect the recombinant protease (rProtease) in TrypLE reagent.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

We recommend using TrypLE Express Enzyme or TrypLE Select Enzyme for passaging mesenchymal stem cells (MSCs). The usage of trypsin will result in lower cell viability upon passage. TrypLE Express and TrypLE Select are defined, animal origin-free, and stable at both room and refrigerated temperatures.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

TrypLE Express Enzyme does not have a His-tag

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

There is no upper limit for TrypLE enzyme incubation. You can incubate your cells with TrypLE longer to detach cells. 30-45 min at 37 degrees C will be usually fine. Or you can consider using TrypLE Select Enzyme (10X) (Cat. No. A1217701 or A1217702 or A1217703) or CTS TrypLE Select Enzyme (Cat. No. A4738001) depending upon the application.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Our TrypLE enzymes are very gentle, and there is no need to use serum or individual proteins to inactivate the enzyme. The TrypLE enzyme activity is inactivated by dilution using the following procedure:

After cell dissociation, transfer the cell suspension to a 15-mL conical tube. Centrifuge at 100 x g for 5-10 min to remove residual enzyme activity. Discard the supernatant and resuspend the cell pellet in 2-5 mL of pre-warmed complete medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations & References (3)

Citations & References
Abstract
Directed differentiation of pancreatic δ cells from human pluripotent stem cells.
Authors:Chen L,Wang N,Zhang T,Zhang F,Zhang W,Meng H,Chen J,Liao Z,Xu X,Ma Z,Xu T,Liu H
Journal:Nature communications
PubMed ID:39068220
Dysfunction of pancreatic δ cells contributes to the etiology of diabetes. Despite their important role, human δ cells are scarce, limiting physiological studies and drug discovery targeting δ cells. To date, no directed δ-cell differentiation method has been established. Here, we demonstrate that fibroblast growth factor (FGF) 7 promotes pancreatic ... More
Non-viral-mediated gene transfer of OX40 ligand for tumor immunotherapy.
Authors:Rakitina OA,Kuzmich AI,Bezborodova OA,Kondratieva SA,Pleshkan VV,Zinovyeva MV,Didych DA,Sass AV,Snezhkov EV,Kostina MB,Koksharov MO,Alekseenko IV
Journal:Frontiers in immunology
PubMed ID:39007148
BACKGROUND: Immune checkpoint blockade (ICB) is rapidly becoming a standard of care in the treatment of many cancer types. However, the subset of patients who respond to this type of therapy is limited. Another way to promote antitumoral immunity is the use of immunostimulatory molecules, such as cytokines or T ... More
A simple tool to improve pluripotent stem cell differentiation.
Authors:Chetty S, Pagliuca FW, Honore C, Kweudjeu A, Rezania A, Melton DA,
Journal:Nat Methods
PubMed ID:23584186
We describe a method to help overcome restrictions on the differentiation propensities of human pluripotent stem cells. Culturing pluripotent stem cells in dimethylsulfoxide (DMSO) activates the retinoblastoma protein, increases the proportion of cells in the early G1 phase of the cell cycle and, in more than 25 embryonic and induced ... More
3 total citations

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