Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Catalog Number | Quantity |
---|---|
12605093 | 5 L |
12605010 | 100 mL |
12605036 | 20 x 100mL |
12605028 | 500 mL |
With |
• Phenol Red |
• EDTA |
Follow the 5 steps to publication-quality fixed-cell imaging.
Download your free 5-step guide today ›
Notice to Purchaser: The purchase of this product conveys to the purchaser the limited, non- transferable right to use the purchased amount of the product for mammailian and insect cell culture only, to (a) perform internal research for the sole benefit of the purchaser; (b) manufacture protein (or other biological material) for resale; and (c) perform research or manufacturing services by the purchaser on a fee for service or contract basis for or on behalf of third parties. However, the purchaser may transfer this product, its components, or materials made using this product to a third party (including contract research/manufacturing organizations), provided that each such third party agrees in writing to use such product, components, or materials solely on behalf of the purchaser, and such third party is restricted from further transferring any such product, components, or materials to any individual or entity other than the purchaser. No additional rights are granted. By purchasing this product, the purchaser agrees not to: (1) resell the product in any form; (2) use the product as a therapeutic agent or diagnostics test component (including, but not limited to, direct use for therapeutic purposes, use as an excipient in finished drug formulations, or use as an active pharmaceutical ingredient); (3) use the product in any applications for direct topical or in vivo use; (4) use the product in any applications for in vitro fertilization; (5) reverse engineer the product or cause the product to be reverse engineered; or (6) use the product for purposes other than what is indicated in this Limited Use Label License. The purchaser is responsible for obtaining all regulatory approvals necessary for any therapeutic or diagnostic use of the protein (or biological material) manufactured using this product. For information on obtaining additional rights, please contact outlicensing@thermofisher.com, Licensing and Commercial Supply, Thermo Fisher Scientific, 5823 Newton Drive, Carlsbad, CA, 92008, United States.
'We offer the Residual TrypLE ELISA Kit (Cat. No. A6533101) that can specifically detect the recombinant protease (rProtease) in TrypLE reagent.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
We recommend using TrypLE Express Enzyme or TrypLE Select Enzyme for passaging mesenchymal stem cells (MSCs). The usage of trypsin will result in lower cell viability upon passage. TrypLE Express and TrypLE Select are defined, animal origin-free, and stable at both room and refrigerated temperatures.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
TrypLE Express Enzyme does not have a His-tag
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
There is no upper limit for TrypLE enzyme incubation. You can incubate your cells with TrypLE longer to detach cells. 30-45 min at 37 degrees C will be usually fine. Or you can consider using TrypLE Select Enzyme (10X) (Cat. No. A1217701 or A1217702 or A1217703) or CTS TrypLE Select Enzyme (Cat. No. A4738001) depending upon the application.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Our TrypLE enzymes are very gentle, and there is no need to use serum or individual proteins to inactivate the enzyme. The TrypLE enzyme activity is inactivated by dilution using the following procedure:
After cell dissociation, transfer the cell suspension to a 15-mL conical tube. Centrifuge at 100 x g for 5-10 min to remove residual enzyme activity. Discard the supernatant and resuspend the cell pellet in 2-5 mL of pre-warmed complete medium.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Share catalog number, name or link