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Gibco™
RPMI 1640 Medium, no glutamine
RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park MemorialRead more
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| Catalog Number | Quantity |
|---|---|
| 21870100 | 6 x 1000 mL |
| 21870076 | 500 mL |
| 21870092 | 10 x 500 mL |
| 21870084 | 1000 mL |
Catalog number 21870100
Price (USD)
278.65
Online Exclusive
314.00Save 35.35 (11%)
Each
Estimated availability date 15-Jul-2025
Quantity:
6 x 1000 mL
Price (USD)
278.65
Online Exclusive
314.00Save 35.35 (11%)
Each
RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This RPMI is modified as follows:
| With | Without |
| • Phenol Red | • HEPES |
| • L-glutamine |
The complete formulation is available.
Using RPMI
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell LineHeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Cell TypeLeukemic Cells
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Product TypeRPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Quantity6 x 1000 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormLiquid
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesPhenol Red
Without AdditivesNo Sodium Pyruvate, No Glutamine, No HEPES
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Media Formulations
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Lot #Certificate TypeDateCatalog Number(s)
3149783Certificate of AnalysisJul 05, 202521870084, 21870076, 21870100
3138592Certificate of AnalysisApr 27, 202521870084, 21870076, 21870100
3096208Certificate of AnalysisMar 07, 202521870084, 21870076, 21870100
2375462Certificate of AnalysisFeb 21, 202521870084, 21870076, 218700250, 21870100, 218701000, 218702500
2537758Certificate of AnalysisFeb 21, 202521870084, 21870076, 218700250, 21870100, 218701000, 218702500
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Citations & References (6)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Fatty acids regulate stress resistance and virulence factor production for Listeria monocytogenes.
Journal:J Bacteriol
PubMed ID:22843841
'Fatty acids (FAs) are the major structural component of cellular membranes, which provide a physical and chemical barrier that insulates intracellular reactions from environmental fluctuations. The native composition of membrane FAs establishes the topological and chemical parameters for membrane-associated functions and is therefore modulated diligently by microorganisms especially in response
Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O.
Journal:Proc Natl Acad Sci U S A
PubMed ID:11248053
'The pore-forming toxin streptolysin O (SLO) can be used to reversibly permeabilize adherent and nonadherent cells, allowing delivery of molecules with up to 100 kDa mass to the cytosol. Using FITC-labeled albumin, 10(5)-10(6) molecules were estimated to be entrapped per cell. Repair of toxin lesions depended on Ca(2+)-calmodulin and on
Novel in vitro and in vivo models and potential new therapeutics to break the vicious cycle of Cryptosporidium infection and malnutrition.
Journal:J Infect Dis
PubMed ID:22454464
Although several animal models of cryptosporidiosis have been reported, most involve genetically or pharmacologically immune-suppressed hosts.
A novel zinc-regulated human zinc transporter, hZTL1, is localized to the enterocyte apical membrane.
Journal:J Biol Chem
PubMed ID:11937503
Zinc is essential to a wide range of cellular processes; therefore, it is important to elucidate the molecular mechanisms of zinc homeostasis. To date, no zinc transporters expressed at the enterocyte apical membrane, and so essential to mammalian zinc homeostasis, have been discovered. We identified hZTL1 as a human expressed
Regulation of Fas-associated death domain interactions by the death effector domain identified by a modified reverse two-hybrid screen.
Journal:J Biol Chem
PubMed ID:12107169
The adapter protein FADD consists of two protein interaction domains and is an essential component of the death inducing signaling complex (DISC) that is formed by activated death receptors of the tumor necrosis factor (TNF) receptor family. The FADD death domain binds to activated receptors such as Fas or other
6 total citations