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Ion Universal Library Quantitation Kit
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Ion Universal Library Quantitation Kit
Thermo Scientific™

Ion Universal Library Quantitation Kit

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Catalog NumberQuantity
A26217250 Reactions
Catalog number A26217
Price (USD)
1,686.00
Each
In stock
Add to cart
Quantity:
250 Reactions
Price (USD)
1,686.00
Each
Add to cart
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The Ion Universal Library Quantitation Kit is an easy-to-use, flexible solution for the accurate quantitation of library input generated by the Ion 16STM Metagenomics Kit for Ion Torrent™ semiconductor sequencing. This kit provides the TaqMan™ assay, TaqMan™ Universal Master Mix, and library standard needed to quantify femtomolar amounts of Ion fragment libraries. With cycling conditions that allow both fast and standard cycling, the Ion Universal Library Quantitation Kit supports most real-time platforms. This real-time PCR kit enables greater accuracy and specificity compared to non-PCR or non-probe-based methods, delivering simplicity and speed to the Ion PGM™ System sequencing workflow.

The Ion Universal Library Quantitation Kit enables:

  • Precision—TaqMan™ assays enable greater specificity and accuracy in detecting properly adapted, amplifiable templates over non-probe-based quantitation methods
  • Sensitivity—the protocol supports quantitation of femtomolar amounts of fragment libraries
  • Simplicity—the validated, ready-to-use qPCR standard requires only a simple serial dilution to determine the optimal concentration of library to use for templated bead preparation
  • Versatility—the highly robust qPCR mix accommodates a wide range of cycling conditions and reaction volumes, and combines highly sensitive detection with a broad quantification range
  • Efficiency—qPCR-based methods use less material for quantification

This kit includes:

  • A TaqMan™ assay designed for Ion library adaptors
  • TaqMan™ Universal PCR Master Mix
  • An adapted E. coli DH10B library control of a defined size constructed using the Ion Fragment Library Kit to yield an adapted library ∼190 bp in size

Note: In addition to use with the Ion 16S™ Metagenomics Kit workflow, the Ion Universal Library Quantitation kit is also compatible with sequencing workflows for amplicons generated using any master mix.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatKit
For Use With (Application)Sequencing
No. of Reactions250 reactions
Product LineIon Torrent™
Product TypeIon Universal Library Quantitation Kit
Quantity250 Reactions
Unit SizeEach
Contents & Storage
• 2 tubes TaqMan Fast Universal Master Mix (1.54 mL per tube); store at 2-8°C.
• Assay mix (250 μL); store at -5 to -30°C.
• 2 tubes E.coli DH10B control library (25 μL per tube); store at -5 to -30°C.
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Frequently asked questions (FAQs)

If I accidentally use the Ion Library TaqMan Quantitation Kit instead of the Ion Universal Library Quantitation Kit with my Ion 16S Metagenomics libraries, what might happen?

This may result in a lower observed library yield as determined by qPCR.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What are the components of the Ion Universal Library Quantitation Kit (Cat. No. A26217)?

The components are listed below:

- TaqMan Fast Universal PCR Master Mix: 2 tubes (1.54 mL per tube)
- Ion Library TaqMan Quantitation Assay, 20X: 250 µL
- E. coli DH10B Ion Control Library: 2 tubes (25 µL per tube)

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How is the Ion Universal Library Quantitation Kit different from the Ion Library TaqMan Quantitation Kit?

The Ion Universal Library Quantitation Kit is compatible with U-containing amplicons generated by the Ion 16S Metagenomics Kit whereas the Ion Library TaqMan Quantitation Kit is not. For most other Ion libraries, use the Ion Library TaqMan Quantitation Kit [exceptions: (1) RNA Seq libraries prepared using the Ion Total RNA-Seq Kit v2 and (2) amplicon libraries prepared following the Ion Amplicon Library Preparation (Fusion Method) User Guide (http://tools.thermofisher.com/content/sfs/manuals/4468326_IonAmpliconLibraryPrep_FusionMethod_UG.pdf), as these libraries have a truncated P1 (trP1) adapter sequence].

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What kit should I use to quantitate library input generated by the Ion 16S Metagenomics Kit?

We recommend using the Ion Universal Library Quantitation Kit, Cat. No. A26217. It is compatible with the uracil-containing amplicons generated by the Ion 16S Metagenomics Kit.

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Lot #Certificate TypeDateCatalog Number(s)
1611012Certificate of AnalysisMay 24, 2021A26217
1809023Certificate of AnalysisOct 24, 2018A26217
1804017Certificate of AnalysisMay 18, 2018A26217
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