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Poly-D-Lysine
Poly-D-Lysine is a chemically synthesized extracellular matrix used to facilitate cell adhesion to tissue culture-treated plastic and glass surfaces. Poly-D-LysineRead more
| Catalog Number | Quantity |
|---|---|
| A3890401 | 100 mL |
Catalog number A3890401
Price (EUR)
125,00
Each
-
Quantity:
100 mL
Price (EUR)
125,00
Each
Poly-D-Lysine is a chemically synthesized extracellular matrix used to facilitate cell adhesion to tissue culture-treated plastic and glass surfaces. Poly-D-Lysine contains polymers with a molecular weight of 50,000–150,000 Daltons, which makes this product ideal for neuronal culture applications. This ready-to-use product is provided as a liquid at a concentration of 0.1 mg/mL. When used on Nunc cell culture plastics with B-27 supplements or the B-27 Plus Neuronal Culture System, Poly-D-Lysine maintains cell viability and normal growth characteristics in multiple neuronal cell types.
Key features include:
• Synthetic and animal origin-free
• Sterile-filtered for cell culture applications
• Validated with B-27 Supplement and B-27 Plus Neuronal Culture System when used with Nunc plastic and glass bottom dishes
Key features include:
• Synthetic and animal origin-free
• Sterile-filtered for cell culture applications
• Validated with B-27 Supplement and B-27 Plus Neuronal Culture System when used with Nunc plastic and glass bottom dishes
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeMammalian Cells
Concentration0.1 mg/mL
Culture TypeMammalian Cell Culture
Product LineGibco™
Purity or Quality GradeCell Culture Grade
Quantity100 mL
Shelf Life12 Months
Shipping ConditionApproved for shipment on Wet Ice
SourceAnimal origin-free, Synthetic
ClassificationAnimal Origin-free
FormLiquid
Product TypeExtracellular Matrix
SterilitySterile
Unit SizeEach
Contents & Storage
Store in refrigerator (2–8°C).
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Figures
Primary rat cortical neurons seeded on Poly-D-Lysine
Recommended Poly-D-Lysine coating volume for different formats of cultureware
Primary rat cortical neurons cultured on Poly-D-Lysine
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Certificates
Search by lot number or partial lot number
Lot #Certificate TypeDateCatalog Number(s)
1021001Certificate of AnalysisJun 09, 2025A3890401
1020999Certificate of AnalysisApr 30, 2025A3890401
1020997Certificate of AnalysisMar 11, 2025A3890401
1020995Certificate of AnalysisFeb 12, 2025A3890401
1020993Certificate of AnalysisFeb 12, 2025A3890401
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Safety Data Sheets
SDSFrequently asked questions (FAQs)
Poly-D-Lysine is in PBS solution.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Yes, Poly-D-Lysine can undergo one freeze-thaw cycle without impacting performance. Please note that this does not extend the shelf-life of the product.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
The Poly-D-Lysine matrix is animal origin-free.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
It is possible that the plates were not adequately rinsed with water prior to plating the cells. Residual Poly-D-Lysine is toxic and may be the cause of cell death. It is recommended to rinse the plates with water three times before exposing the cells to the matrix.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
When coating the surface with Poly-D-Lysine, make sure that the entire surface is fully covered and that the appropriate volume of coating matrix is applied.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.