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RiboPure™ RNA Purification Kit, yeast
For the isolation of extremely high-quality RNA from yeast without the use of enzymatic pretreatments that may alter gene expressionRead more
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| Catalog Number | Quantity |
|---|---|
| AM1926 | 50 Preps |
Catalog number AM1926
Price (USD)
670.65
Online Exclusive
700.00Save 29.35 (4%)
Each
Estimated availability date Pending
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Quantity:
50 Preps
Price (USD)
670.65
Online Exclusive
700.00Save 29.35 (4%)
Each
For the isolation of extremely high-quality RNA from yeast without the use of enzymatic pretreatments that may alter gene expression profiles. Each Ambion™ kit includes sufficient reagents for 50 purifications each from 107 to 108 yeast cells.
• Highest yields of total RNA available
• No enzymatic pretreatment required
• Includes DNA-free™ Reagents
It has traditionally been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells. Many protocols use enzymatic pretreatments that may affect gene expression patterns. The RiboPure™-Yeast RNA Isolation Kit uses no enzymatic pretreatments and thus does not alter expression profiles. It combines a simple and efficient glass bead/one step phenol–mediated disruption using Ambion's Vortex Bead Beater Adapter (see Accessory Products) followed by a glass fiber filter– based RNA purification step. After DNase treatment with the included DNA-free™ Reagents, the resulting RNA is exceptionally pure containing little to no genomic DNA (less than 0.05%). The kit has been used extensively at Ambion to isolate high-quality RNA from Saccharomyces cerevisiae, Schizosaccharomyces pombe, Pichia pastoris, and Candida albicans (see Figure). The kit provides the highest yield of any method tested from each of these species (see Figure).
Accessory Products:
The Kit requires the use of the Vortex Adapter (Cat. No. AM10024), which turns a standard laboratory vortex into a bead beater. This adapter fits the Vortex Genie™ 2 and holds 12 tubes.
• Highest yields of total RNA available
• No enzymatic pretreatment required
• Includes DNA-free™ Reagents
It has traditionally been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells. Many protocols use enzymatic pretreatments that may affect gene expression patterns. The RiboPure™-Yeast RNA Isolation Kit uses no enzymatic pretreatments and thus does not alter expression profiles. It combines a simple and efficient glass bead/one step phenol–mediated disruption using Ambion's Vortex Bead Beater Adapter (see Accessory Products) followed by a glass fiber filter– based RNA purification step. After DNase treatment with the included DNA-free™ Reagents, the resulting RNA is exceptionally pure containing little to no genomic DNA (less than 0.05%). The kit has been used extensively at Ambion to isolate high-quality RNA from Saccharomyces cerevisiae, Schizosaccharomyces pombe, Pichia pastoris, and Candida albicans (see Figure). The kit provides the highest yield of any method tested from each of these species (see Figure).
Accessory Products:
The Kit requires the use of the Vortex Adapter (Cat. No. AM10024), which turns a standard laboratory vortex into a bead beater. This adapter fits the Vortex Genie™ 2 and holds 12 tubes.
WARNING: Cancer and Reproductive Harm – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Elution Volume50–100 μL
Final Product TypeTotal RNA
For Use With (Application)RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
High-throughput CompatibilityNot High-throughput Compatible (Manual)
Purification Time1.5 hr. (Including DNase treatment)
Quantity50 Preps
Shipping ConditionBox 1: Dry Ice
Box 2: Room Temperature
Box 2: Room Temperature
Starting Material AmountUp to 108 cells
YieldUp to 300 μg
Isolation TechnologySpin Column (Glass Fiber Filter)
Sample TypeYeast
Unit SizeEach
Contents & Storage
• 50 1.5-mL Screw Cap Tubes; store at room temperature
• 50 Filter Cartridges; room temperature
• 100 Collection Tubes (2 mL); room temperature
• 24 mL Lysis Buffer; 4°C
• 2.4 mL 10% SDS; 4°C
• 24 mL Phenol:Chloroform:IAA; 4°C
• 95 mL Binding Buffer; 4°C
• 35 mL Wash Solution 1; 4°C
• 50 mL Wash Solution 2/3 (concentrated); 4°C
• 5 mL Elution Solution; 4°C
• 80 g Zirconia Beads; -20°C
• 550 μL 10X DNase 1 Buffer; -20°C
• 200 μL DNase 1; -20°C
• 550 μL DNase Inactivation Reagent; -20°C
• 50 Filter Cartridges; room temperature
• 100 Collection Tubes (2 mL); room temperature
• 24 mL Lysis Buffer; 4°C
• 2.4 mL 10% SDS; 4°C
• 24 mL Phenol:Chloroform:IAA; 4°C
• 95 mL Binding Buffer; 4°C
• 35 mL Wash Solution 1; 4°C
• 50 mL Wash Solution 2/3 (concentrated); 4°C
• 5 mL Elution Solution; 4°C
• 80 g Zirconia Beads; -20°C
• 550 μL 10X DNase 1 Buffer; -20°C
• 200 μL DNase 1; -20°C
• 550 μL DNase Inactivation Reagent; -20°C
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Figures
Yeast Total RNA Isolated Using the RiboPure-Yeast Kit
RNA Yields From 3 Yeast Species Using Ambion's RiboPure-Yeast or Competitors' Kits
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Safety Data Sheets
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Frequently asked questions (FAQs)
The column cut-off size is 200 nt. Using a RiboPure kit is not appropriate for miRNA recovery. For miRNA isolation, we would suggest using a mirVana kit, the TRIzol Plus RNA Purification Kit, or the PureLink RNA Micro Kit.
The PureLink RNA Mini Kit provides rapid column-based purification of total RNA, without organic lysis (phenol/chloroform). You can obtain up to 1,000 µg of purified RNA from a single extraction. The RiboPure RNA Purification Kits combine a phenol/guanidine thiocyanate solution with a glass-fiber filter purification method.