GeneJET Plasmid Miniprep Kit
For better long-term performance store purification columns at 2°C to 8°C.
GeneJET Plasmid Miniprep Kit
Thermo Scientific™

GeneJET Plasmid Miniprep Kit

Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column.
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Catalog NumberQuantity
K050250 Preps
K0503250 Preps
Catalog number K0502
Price (USD)
80.65
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96.00
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Quantity:
50 Preps
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Price (USD)
80.65
Online Exclusive
96.00
Save 15.35 (16%)
Each
Add to cart
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Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. The kit recovers up to 20 μg of high copy plasmid DNA per isolation procedure.

Highlights

  • Efficient—high yields of up to 20 μg of high quality plasmid DNA
  • Fast—procedure takes less than 14 minutes
  • Convenient—no phenol/chloroform extraction or alcohol precipitation required
  • Pure—purified DNA is immediately ready to use

Applications

Fast isolation of high purity plasmid DNA suitable for all conventional molecular biology procedures, including:

  • FastDigest or conventional restriction digestion
  • Automated fluorescent and radioactive sequencing
  • PCR
  • In vitro transcription
  • Transformation

Principle

A bacterial culture is harvested and lysed. The lysate is then cleared by centrifugation and applied on the silica column to selectively bind DNA molecules at a high salt concentration. The adsorbed DNA is washed to remove contaminants, and the pure plasmid DNA is eluted in a small volume of elution buffer or water. The purified DNA is ready for immediate use in all molecular biology procedures, such as fast and conventional digestion with restriction enzymes, PCR, in vitro transcription, transformation, and automated sequencing.

Components

  • Resuspension Solution
  • Lysis Solution
  • Neutralization Solution Wash Solution (concentrated)
  • RNase A Solution
  • Elution Buffer
  • Spin Columns
  • Collection Tubes
  • Detailed Protocol

Related Products

  • RNase A (Part No.: R1253)
  • Elution Buffer for GeneJET Plasmid Miniprep Kit (Part No.: R1263)
  • Lysis Solution for GeneJET Plasmid Miniprep Kit (Part No.: R1223)
  • Resuspension Solution for GeneJET Plasmid Miniprep Kit (Part No.: R1213)
  • Neutralization Solution for GeneJET Plasmid Miniprep Kit (Part No.: R1233)
  • Wash Solution for GeneJET Plasmid Miniprep Kit (Part No.: R1243)
  • Binding Buffer for GeneJET Plasmid Miniprep Kit(Part No.: R1331)
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Column TypeSpin Column
For Use With (Application)PCR, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, In Vitro Transcription
High-throughput CompatibilityCompatible (Vacuum manifold)
Prep Scale< 100 μg (Small-Scale) Plasmid DNA
Product LineGeneJET
Product TypePlasmid MiniPrep Kit
Quantity50 Preps
Sample TypeBacteria
ScaleMini
TargetPlasmid DNA
Test Time<14 min
FormatSpin Column
Isolation TechnologySilica Column
Unit SizeEach
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Frequently asked questions (FAQs)

Can I use water instead of Elution Buffer in the elution step of GeneJET and MagJET nucleic acid purification kits?

We would highly recommend using the provided Elution Buffer in the elution step for best results. You may use nuclease-free water; however, it may affect the yield of the nucleic acids during purification and their stability during storage.

Are MagJET and GeneJET plasmid purification kits compatible with EndA+ bacterial strains?

Yes, MagJET and GeneJET Plasmid DNA Purification Kits are suitable for plasmid DNA purification from EndA+ strains. Please refer to the specific kit protocol for details. GeneJET Plasmid Miniprep Kit requires Wash Solution I (Cat. No. R1611, not included in the kit) for EndA+ strains.

Can I use a vacuum manifold with GeneJET kits?

The GeneJET spin columns are compatible with vacuum manifolds of standard size (e.g. Qiagen, Sigma-Aldrich, Promega). However, not all GeneJET kits are supported with a vacuum protocol. Please refer to the particular kit protocol for the detailed procedure.

At what temperature should I perform centrifugation steps in the GeneJET kits?

We recommend performing centrifugation steps at room temperature when the temperature is not specified.

Are GeneJET spin columns or MagJET magnetic beads available separately?

No, we do not offer GeneJET columns or MagJET magnetic beads separately at this time.

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3272406Certificate of AnalysisJul 02, 2025K0503
3270029Certificate of AnalysisJun 27, 2025K0503
3269844Certificate of AnalysisJun 27, 2025K0503
3262525Certificate of AnalysisJun 17, 2025K0502
3257799Certificate of AnalysisJun 12, 2025K0503
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