Countess™ Cell Counting Chamber Slides and Holder, disposable
Experience nuclei counting with fluorescent-mode counting algorithms, simplified workflows, built-in dilution calculators and reports using the latest version of Countess software with optional 21 CFR Part 11 compliance. Download now!
Countess™ Cell Counting Chamber Slides and Holder, disposable
Invitrogen™

Countess™ Cell Counting Chamber Slides and Holder, disposable

Countess Cell Counting Chamber Slides are conveniently packaged for use with the Countess Automated Cell Counter. Each disposable slide features two enclosed chambers. All Countess Cell Counting Chamber Slides (except Cat. No. C10283) are available with vials of trypan blue.
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Catalog NumberTypeQuantity
T10282Trypan Blue Stain2 x 1 mL
C10228Cell Counting Chamber Slide50 Slides
C10312Cell Counting Chamber Slide500 Slides
C10313Cell Counting Chamber Slide1250 Slides
C10314Cell Counting Chamber Slide2500 Slides
C10315Cell Counting Chamber Slide5000 Slides
AMEP4745Slide Holder1 unit, 1 unit, 1 unit, 1 unit
C10283Cell Counting Chamber Slide50 Slides
Catalog number T10282
Price (USD)
42.65
Online Exclusive
44.54
Save 1.89 (4%)
2 x 1 mL
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Type:
Trypan Blue Stain
Quantity:
2 x 1 mL
Recurring order eligible. Learn more »
Price (USD)
42.65
Online Exclusive
44.54
Save 1.89 (4%)
2 x 1 mL
Add to cart
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Countess Cell Counting Chamber Slides are designed exclusively for automated cell counting on the Countess Automated Cell Counter. These individually packaged slides feature two enclosed chambers and are disposable, offering convenience when performing cell culture. The Countess family of automated cell counters employs the widely used trypan blue staining method for cell counting to identify dead cells and measure cellular viability using brightfield.

The disposable Countess Cell Counting Chamber Slides offer convenience in automated cell counting for processing multiple cell samples, busy cell culture facilities, and easy disposal of contaminated or hazardous samples. They also eliminate the risk of contamination from residual samples. After use, Countess slides can be disposed of as standard biohazard waste. Unlike a hemocytometer, there is no need to clean Countess slides before or after use.

Each Countess Cell Counting Chamber Slide has two separate, enclosed chambers for counting one sample in duplicate or two different samples. Cell counting occurs in a central location of the counting chamber. The volume counted is 0.4 μL, the same as counting four 1-mm2 squares in a standard hemocytometer.

The Countess Automated Cell Counter works in three simple steps:

  • Mix 10 μL of sample with 10 μL of trypan blue stain or other desired reagent, including ReadyCount™ stains, and pipet into a disposable Countess chamber slide
  • Insert the slide into the instrument
  • Press 'Count'

That's it—get the total cell count, percent viable cells, and average cell size in as little as 30 seconds.

The Countess Cell Counting Chamber Slides include two vials of trypan blue for brightfield automated cell counting and viability analysis (Cat. Nos. C10228, C10312, C10314, C10313, and C10315). The slides can also be purchased as 1 box of 50 slides without trypan blue (Cat. No. C10283) for use with other stains such as the ReadyCount fluorescent stains. The trypan blue vials are also available for purchase without slides (Cat. No. T10282).

Trypan blue staining is a long-standing and widely used method to measure cellular viability by identifying dead cells. Live cells with intact membranes exclude the dye, while dead cells with compromised membranes become stained, allowing for the differentiation and counting of live and dead cells to assess cellular viability. Trypan blue staining is the method employed by the Countess family of automated cell counters for cell counting, and this 0.4% trypan blue solution is the correct concentration for use on these instruments.

Note: Precipitates are quite common in trypan blue, and typically increase with storage time and freezing. The three approaches below may help minimize trypan blue precipitate in your cell counting samples:

  • Sterile filtration of trypan blue using 0.3-micron filter
  • Centrifugation or passive settling (avoid mixing or vortexing trypan stock solutions)
  • Gentle warming of the 1-mL trypan solution to 37°C for 10 minutes

The Countess Disposable Slide Holder is used to hold the Countess disposable slides. It is designed for use with Countess II FL, Countess 3, and Countess 3 FL automated cell counters and is included with these cell counters. A replacement Countess Disposable Slide Holder (Cat. No. AMEP4745 ) is available for separate purchase. This Countess Disposable Slide Holder is not compatible with the Countess Reusable Slide (Cat. No. A25750).

WARNING: Cancer – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorBlue
Detection MethodColorimetric
For Use With (Equipment)Countess Automated Cell Counter
Label or DyeTrypan Blue
Quantity2 x 1 mL
Shipping ConditionRoom Temperature
TypeTrypan Blue Stain
Unit Size2 x 1 mL
Contents & Storage
Trypan blue stain, 0.4% solution, 2 x 1 mL vials

Store at room temperature.
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Frequently asked questions (FAQs)

Why am I getting a much higher bead count on the Countess 3 Standard Slide (Cat. No. A51876)?

When counting cells that have been mixed with Trypan Blue, the Countess accounts for this 1:1 dilution and multiplies the count by 2.
When using the Countess 3 Standard Slide, the Trypan Blue correction box needs to be unchecked, since a 1:1 dilution was not made with Trypan Blue.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is there a limit to the number of times I can warm up Trypan Blue Stain (0.4%) for use with the Countess Automated Cell Counter (Cat. No. T10282)? What is the importance of warming up in aliquots of 1 mL?

The Trypan Blue Stain (0.4%) can be warmed up to 37 degrees C numerous times. The entire 1 mL volume of the stain should be warmed up to ensure that you are getting the entire amount at the proper concentration of 0.4%; if you took out an aliquot to warm up, and left behind some precipitate, the aliquot that is warmed up may not contain the complete amount of dye and the final concentration may be less than 0.4%.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the shelf life of Trypan Blue Stain (0.4%) for use with the Countess Automated Cell Counter?

The shelf life of the product is 36 months from the date of production.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can cells be exposed to trypan blue before all cells are stained with the dye?

Unfortunately, there is no definitive answer to this question. The rate at which the cell-impermeant dye is absorbed depends on the cell type, their state of health, nourishment, engulfment activity, etc.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What causes trypan blue to form a precipitate?

Exposure to light may degrade the dye and these contaminants may promote precipitation. Trypan blue can also form orange/red fibrous aggregates if exposed to refrigeration or freezing temperatures.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

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Lot #Certificate TypeDateCatalog Number(s)
3192626Certificate of AnalysisJun 29, 2025T10282
3174415Certificate of AnalysisJun 29, 2025T10282
3192625Certificate of AnalysisJun 20, 2025T10282
3174420Certificate of AnalysisJun 20, 2025T10282
3158433Certificate of AnalysisJun 17, 2025T10282
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Citations & References (1)

Citations & References
Abstract
Role of hepatic transporters in the disposition and hepatotoxicity of a HER2 tyrosine kinase inhibitor CP-724,714.
Authors:Feng B, Xu JJ, Bi YA, Mireles R, Davidson R, Duignan DB, Campbell S, Kostrubsky VE, Dunn MC, Smith AR, Wang HF,
Journal:Toxicol Sci
PubMed ID:19223659
'CP-724,714, a potent and selective orally active HER2 tyrosine kinase inhibitor, was discontinued from clinical development due to unexpected hepatotoxicity in cancer patients. Based on the clinical manifestation of the toxicity, CP-724,714 likely exerted its hepatotoxicity via both hepatocellular injury and hepatobiliary cholestatic mechanisms. The direct cytotoxic effect, hepatobiliary disposition ... More
1 total citations

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