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Gibco™
TrypLE™ Express Enzyme (1X), no phenol red
TrypLE Express is an animal origin-free, recombinant enzyme used for dissociating a wide range of adherent mammalian cells, including CHO,Read more
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| Catalog Number | Quantity |
|---|---|
| 12604013 | 100 mL |
| 12604021 | 500 mL |
| 12604039 | 20 x 100mL |
| 12604054 | 5 L |
Catalog number 12604013
Price (CNY)
380.00
Each
In stock
Quantity:
100 mL
Price (CNY)
380.00
Each
TrypLE Express is an animal origin-free, recombinant enzyme used for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. TrypLE Express cleaves peptide bonds on the C-terminal sides of lysine and arginine, and is a direct replacement for trypsin. Its exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts. We offer a variety of TrypLE formulations for a range of cell culture applications. Compared to trypsin and other dissociation reagents, TrypLE Express is:
• Gentle on cells
• Room temperature-stable
• Easy to use
• Animal origin-free (AOF)
The formulation of TrypLE solutions is available, although we keep the enzyme concentration confidential.
Gentle on cells
TrypLE Express' exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.
Room temperature-stable
TrypLE Express remains stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.
Easy to use
TrypLE Express can be directly substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE Express, avoiding the need for trypsin inhibitors, such as FBS.
Animal origin-free (AOF)
Unlike porcine trypsin, TrypLE Express is free of animal-derived components.
cGMP manufacturing and quality system
For supply chain continuity, we manufacture Gibco TrypLE Express Enzyme (1X), no phenol red at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements and are certified to the ISO 13485 standard.
• Gentle on cells
• Room temperature-stable
• Easy to use
• Animal origin-free (AOF)
This TrypLE Express is modified as follows:
| With | Without |
| • EDTA | • Phenol Red |
The formulation of TrypLE solutions is available, although we keep the enzyme concentration confidential.
Gentle on cells
TrypLE Express' exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.
Room temperature-stable
TrypLE Express remains stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.
Easy to use
TrypLE Express can be directly substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE Express, avoiding the need for trypsin inhibitors, such as FBS.
Animal origin-free (AOF)
Unlike porcine trypsin, TrypLE Express is free of animal-derived components.
cGMP manufacturing and quality system
For supply chain continuity, we manufacture Gibco TrypLE Express Enzyme (1X), no phenol red at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements and are certified to the ISO 13485 standard.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeMammalian Cells
ChelatorsEDTA
Concentration1 X
Culture TypeAdherent Cell Culture
Green FeaturesEnergy efficient, Sustainable packaging
OsmolalityCheck and Record
Product LineGibco™
Quantity100 mL
RemarksRoom Temperature Stable
Shelf Life24 Months
Shipping ConditionRoom Temperature
SourceAnimal origin-free
Tested ForIn vitro Bioassay
ClassificationAnimal Origin-free
FormLiquid
Product TypeCell Culture Dissociation Reagent
SterilitySterile-filtered
Without AdditivesNo Phenol Red
pH6 to 8
Unit SizeEach
Contents & Storage
Storage conditions: 15°C to 30°C, protect from light
Alternate storage conditions: 2°C to 8°C or -5°C to -20°C
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture
Alternate storage conditions: 2°C to 8°C or -5°C to -20°C
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture
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Lot #Certificate TypeDateCatalog Number(s)
3203929Certificate of AnalysisJul 05, 202512604054, 12604021, 12604013
3171015Certificate of AnalysisJun 29, 202512604054, 12604021, 12604013
3139331Certificate of AnalysisJun 19, 202512604054, 12604021, 12604013
3108154Certificate of AnalysisJun 01, 202512604054, 12604021, 12604013
3139810Certificate of AnalysisMay 29, 202512604054, 12604021, 12604013
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Product Information
Limited Use Label Licenses (LULL)
'
Notice to Purchaser: The purchase of this product conveys to the purchaser the limited, non- transferable right to use the purchased amount of the product for mammailian and insect cell culture only, to (a) perform internal research for the sole benefit of the purchaser; (b) manufacture protein (or other biological material) for resale; and (c) perform research or manufacturing services by the purchaser on a fee for service or contract basis for or on behalf of third parties. However, the purchaser may transfer this product, its components, or materials made using this product to a third party (including contract research/manufacturing organizations), provided that each such third party agrees in writing to use such product, components, or materials solely on behalf of the purchaser, and such third party is restricted from further transferring any such product, components, or materials to any individual or entity other than the purchaser. No additional rights are granted. By purchasing this product, the purchaser agrees not to: (1) resell the product in any form; (2) use the product as a therapeutic agent or diagnostics test component (including, but not limited to, direct use for therapeutic purposes, use as an excipient in finished drug formulations, or use as an active pharmaceutical ingredient); (3) use the product in any applications for direct topical or in vivo use; (4) use the product in any applications for in vitro fertilization; (5) reverse engineer the product or cause the product to be reverse engineered; or (6) use the product for purposes other than what is indicated in this Limited Use Label License. The purchaser is responsible for obtaining all regulatory approvals necessary for any therapeutic or diagnostic use of the protein (or biological material) manufactured using this product. For information on obtaining additional rights, please contact outlicensing@thermofisher.com, Licensing and Commercial Supply, Thermo Fisher Scientific, 5823 Newton Drive, Carlsbad, CA, 92008, United States.
'Protocols
Citations & References (7)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
A comparison between different human hepatocyte models reveals profound differences in net glucose production, lipid composition and metabolism in vitro.
Journal:Experimental cell research
PubMed ID:38499143
Method for Large-scale Production of hIPSC Spheroids.
Journal:Bio-protocol
PubMed ID:38618177
Stem cell spheroids are rapidly becoming essential tools for a diverse array of applications ranging from tissue engineering to 3D cell models and fundamental biology. Given the increasing prominence of biotechnology, there is a pressing need to develop more accessible, efficient, and reproducible methods for producing these models. Various techniques
Isolation and cultivation of human keratinocytes from skin or plucked hair for the generation of induced pluripotent stem cells.
Journal:Nat Protoc
PubMed ID:20134422
'The ease of generating induced pluripotent stem (iPS) cells, and possibly their properties after reprogramming, depends on the origin of the somatic cell starting population. Reprogramming of keratinocytes is both faster and more efficient compared with fibroblasts, although more care is required when isolating, culturing and infecting these cells. In
A simple tool to improve pluripotent stem cell differentiation.
Journal:Nat Methods
PubMed ID:23584186
We describe a method to help overcome restrictions on the differentiation propensities of human pluripotent stem cells. Culturing pluripotent stem cells in dimethylsulfoxide (DMSO) activates the retinoblastoma protein, increases the proportion of cells in the early G1 phase of the cell cycle and, in more than 25 embryonic and induced
VCAM-1 expression in adult hematopoietic and nonhematopoietic cells is controlled by tissue-inductive signals and reflects their developmental origin.
Journal:Blood
PubMed ID:15769895
Although expression of vascular cell adhesion molecule 1 (VCAM-1) in endothelial cells and its functional implications have been previously appreciated, VCAM-1 expression in other than endothelial cells, especially hematopoietic cells, has been recently recognized and has not been explored in detail. Using normal mice and mice with a conditional ablation
7 total citations