Trypan Blue Solution, 0.4%
Trypan Blue Solution, 0.4%
Gibco™

Trypan Blue Solution, 0.4%

Trypan Blue Solution, 0.4%, is routinely used as a cell stain to assess cell viability using the dye exclusion test.Read more
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Catalog NumberQuantity
15250061Promo Image100 mL
Catalog number 15250061
Price (CNY)
413.00
Each
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Quantity:
100 mL
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Price (CNY)
413.00
Each
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Trypan Blue Solution, 0.4%, is routinely used as a cell stain to assess cell viability using the dye exclusion test. This test is often performed while counting cells with the hemocytometer during routine subculturing, but can be performed any time cell viability needs to be determined quickly and accurately. The dye exclusion test is based upon the concept that viable cells do not take up impermeable dyes (like Trypan Blue), but dead cells are permeable and take up the dye.

cGMP manufacturing and quality system
Trypan Blue Solution is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeMammalian Cells
ColorBlue
Concentration0.004
Detection MethodColorimetric
For Use With (Application)Cell Counting
Quantity100 mL
Shelf Life36 Months
Shipping ConditionRoom Temperature
FormLiquid
Product TypeTrypan Blue
SterilitySterile-filtered
Unit SizeEach
Contents & Storage
Storage conditions: 15 – 30°C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
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Certificates

Lot #Certificate TypeDateCatalog Number(s)
3118238Certificate of AnalysisJul 03, 202515250061
3118236Certificate of AnalysisJun 26, 202515250061
3118234Certificate of AnalysisMay 17, 202515250061
3097467Certificate of AnalysisApr 04, 202515250061
3064263Certificate of AnalysisMar 13, 202515250061
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Safety Data Sheets

Frequently asked questions (FAQs)

There are a number of reagents used to quench fluorescence on the outside of live cells or in the media (background fluorescence). Our Backdrop Background Suppressor reagents are a great option to quench blue, green, and red fluorescent dyes, various media components and fluorescent proteins. Another commonly used reagent is the cell impermeant colorimetric dye, Trypan blue.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Trypan Blue is filter sterilized through a 0.22 µm filter.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

It is a 0.85% saline solution.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Unfortunately, there is no definitive answer to this question. The rate at which the cell-impermeant dye is absorbed depends on the cell type, their state of health, nourishment, engulfment activity, etc.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Exposure to light may degrade the dye and these contaminants may promote precipitation. Trypan blue can also form orange/red fibrous aggregates if exposed to refrigeration or freezing temperatures.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (3)

Citations & References
Abstract
Protocol to generate human liver spheroids to study liver fibrosis induced by metabolic stress.
Authors:Kim HY,Lee W,Liu X,Jang H,Sakane S,Carvalho-Gontijo Weber R,Diggle K,Kerk SA,Metallo CM,Kisseleva T,Brenner DA
Journal:STAR protocols
PubMed ID:38833372
Currently, there is no effective treatment for obesity and alcohol-associated liver diseases, partially due to the lack of translational human models. Here, we present a protocol to generate 3D human liver spheroids that contain all the liver cell types and mimic “livers in a dish.” We describe strategies to induce ... More
Bax and Bak independently promote cytochrome C release from mitochondria.
Authors: Degenhardt Kurt; Sundararajan Ramya; Lindsten Tullia; Thompson Craig; White Eileen;
Journal:J Biol Chem
PubMed ID:11836241
'Pro-apoptotic Bax and Bak have been implicated in the regulation of p53-dependent apoptosis. We assessed the ability of primary baby mouse kidney (BMK) epithelial cells from bax(-/-), bak(-/-), and bax(-/-) bak(-/-) mice to be transformed by E1A alone or in conjunction with dominant-negative p53 (p53DD). Although E1A alone transformed BMK ... More
Hypoxia and Nitric Oxide Treatment Confer Tolerance to Glucose Starvation in a 5'-AMP-activated Protein Kinase-dependent Manner.
Authors: Esumi Hiroyasu; Izuishi Kunihiko; Kato Kazuyoshi; Hashimoto Koichi; Kurashima Yukiko; Kishimoto Atsuhiro; Ogura Tsutomu; Ozawa Takayuki;
Journal:J Biol Chem
PubMed ID:12091379
Hypoxia is a critical event for higher organisms, and cells and tissues react by increasing the oxygen supply by vasodilatation, angiogenesis, and erythropoiesis and maintaining cellular energy by increasing glycolysis and inhibiting anabolic pathways. Stimulation of glycolysis has been regarded as the main response that increases energy production during hypoxia; ... More
3 total citations

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