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Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)
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Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)
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Thermo Scientific™

Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)

Thermo Scientific Halt Protease and Phosphatase Inhibitor Cocktail (100X) provides the convenience of a single solution with full protein sampleRead more
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Catalog NumberQuantity
784401 mL
7844224 x 100 μL
7844610 mL
784445 x 1 mL
Catalog number 78440
Price (CNY)
3,049.00
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Ends: 31-Dec-2025
4,125.00
Save 1,076.00 (26%)
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Quantity:
1 mL
Recurring order eligible. Learn more »
Request bulk or custom format
Price (CNY)
3,049.00
Online Exclusive
Ends: 31-Dec-2025
4,125.00
Save 1,076.00 (26%)
Each
Add to cart
Ask our AI about this Product
Thermo Scientific Halt Protease and Phosphatase Inhibitor Cocktail (100X) provides the convenience of a single solution with full protein sample protection for cell and tissue lysates.

Features of Halt Protease and Phosphatase Inhibitor Cocktail (100X):

• Complete protection—all-in-one cocktail contains both protease and phosphatase inhibitors
• Multiple package sizes—choose from four convenient packages sizes to match nearly any scale
• Convenient —the 100X liquid formulation is much easier and more effective to use than individual inhibitors and other formats
• Easy storage—aqueous-based format allows for convenient cold-room storage without freezing, by contrast with DMSO-based cocktails
• Compatible—can be used in mass spectrometry (MS) because it does not contain AEBSF, which can cause peaks to shift
• Validated—fully compatible with Thermo Scientific Pierce Cell Lysis Buffers

Halt Protease and Phosphatase Inhibitor Cocktail is specifically optimized to protect proteins from degradation during extraction and purification. The cocktail contains inhibitors against the major classes of proteases and phosphatases, targeting aminopeptidases, cysteine and serine proteases and serine/threonine and protein tyrosine phosphatases. To promote the inhibition of metalloproteases, 0.5M EDTA is provided in a separate tube.

Related Products
Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)
For Research Use Only. Not for use in diagnostic procedures.
Specifications
BufferLysis Buffer
InhibitorsProtease Inhibitor, Phosphatase Inhibitor
Quantity1 mL
Reagent TypeProtease Inhibitor, Phosphatase Inhibitor
FormLiquid
Product LineHalt™
Product TypeProtease and Phosphatase Inhibitor Cocktail
Unit SizeEach
Contents & Storage
Upon receipt store at 4°C
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Frequently asked questions (FAQs)

Can I store the 1X solution of the Halt Protease and Phosphatase Inhbitor Cocktail and if so, for how long?

The stability of the 1X solution is undetermined. For best results, prepare solutions just before use. The 100X concentrate is stable for at least one year stored at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are the concentrations of the various components of the combined Halt Protease and Phosphatase Inhibitor Cocktail?

Although we reveal the identity of the inhibitors in the combined cocktail, their concentrations are proprietary.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Do you offer the components of the Halt Protease and Phosphatase Cocktail individually?

Yes. Halt Protease, Halt Phosphatase and Protease/Phosphatase combined Inhibitor Cocktails are available. In addition, some of the individual inhibitors of the Halt Protease and Phosphatase Inhibitor Cocktail are offered separately.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

When do I need to add EDTA when using the Halt Protease and Phosphatase Cocktail?

EDTA chelates the metal ions required for metalloprotease activity. Add EDTA if metalloproteases are likely to adversely affect target protein function; however, avoid using EDTA when the downstream application involves metal chelate affinity purification or the target protein requires metals for proper function.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How much of the Halt Protease and Phosphatase Cocktail do I use for my sample?

The Halt Protease and Phosphatase Cocktail is supplied at a 100X concentrate and is used at 1X. For example, add 10µL of 100X Halt Cocktail to 1mL of sample. Some samples, however, contain high levels of proteases or phosphatase and might require a more concentrated treatment (i.e., 2-3X).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all Halt™ Protease and Phosphatase Inhibitor Cocktail (100X) FAQs

Figures

Protease and phosphatase inhibitors included in the cocktail formulation and their targeted enzyme class
Protease and phosphatase inhibitors included in the cocktail formulation and their targeted enzyme class

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3215602Certificate of AnalysisJul 09, 202578440
3215606Certificate of AnalysisJul 09, 202578446
3214001ACertificate of AnalysisJul 09, 202578444
3214001Certificate of AnalysisJul 09, 202578444
3214918Certificate of AnalysisJul 09, 202578440
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Citations & References (16)

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Citations & References
Abstract
A guide to selecting high-performing antibodies for human Midkine for use in Western blot and immunoprecipitation.
Authors:Ayoubi R,Southern K,Laflamme C,NeuroSGC/YCharOS Collaborative Group
Journal:F1000Research
PubMed ID:39092005
Midkine is a secreted protein that acts as a growth factor or cytokine involved in cell survival and inflammatory processes. It accumulates in amyloid plaques, which are hallmarks of Alzheimer's Disease (AD). The reproducibility of Midkine research would be enhanced if the community had access to well-characterized anti-Midkine antibodies. In ... More
Chemoproteomic discovery of a covalent allosteric inhibitor of WRN helicase.
Authors:Baltgalvis KA,Lamb KN,Symons KT,Wu CC,Hoffman MA,Snead AN,Song X,Glaza T,Kikuchi S,Green JC,Rogness DC,Lam B,Rodriguez-Aguirre ME,Woody DR,Eissler CL,Rodiles S,Negron SM,Bernard SM,Tran E,Pollock J,Tabatabaei A,Contreras V,Williams HN,Pastuszka MK,Sigler JJ,Pettazzoni P,Rudolph MG,Classen M,Brugger D,Claiborne C,Plancher JM,Cuartas I,Seoane J,Burgess LE,Abraham RT,Weinstein DS,Simon GM,Patricelli MP,Kinsella TM
Journal:Nature
PubMed ID:38658751
WRN helicase is a promising target for treatment of cancers with microsatellite instability (MSI) due to its essential role in resolving deleterious non-canonical DNA structures that accumulate in cells with faulty mismatch repair mechanisms(1-5). Currently there are no approved drugs directly targeting human DNA or RNA helicases, in part owing ... More
Selection of Leptin Surrogates by a General Phenotypic Screening Method for Receptor Agonists.
Authors:Wang T,Chen X,Yang G,Shi X
Journal:Biomolecules
PubMed ID:38672473
There is a high demand for agonist biomolecules such as cytokine surrogates in both biological and medicinal research fields. These are typically sourced through natural ligand engineering or affinity-based screening, followed by individual functional validation. However, efficient screening methods for identifying rare hits within immense libraries are very limited. In ... More
Host E3 ubiquitin ligase ITCH mediates Toxoplasma gondii effector GRA35-triggered NLRP1 inflammasome activation and cell-autonomous immunity.
Authors:Wang Y,Hollingsworth LR,Sangaré LO,Paredes-Santos TC,Krishnamurthy S,Penn BH,Wu H,Saeij JPJ
Journal:mBio
PubMed ID:38376248
Toxoplasma gondii is an intracellular parasite that can activate the NLRP1 inflammasome leading to macrophage pyroptosis in Lewis rats, but the underlying mechanism is not well understood. In this study, we performed a genome-wide CRISPR screen and identified the dense granule proteins GRA35, GRA42, and GRA43 as the Toxoplasma effectors ... More
LARP1 binds ribosomes and TOP mRNAs in repressed complexes.
Authors:Saba JA,Huang Z,Schole KL,Ye X,Bhatt SD,Li Y,Timp W,Cheng J,Green R
Journal:The EMBO journal
PubMed ID:39533057
Terminal oligopyrimidine motif-containing mRNAs (TOPs) encode all ribosomal proteins in mammals and are regulated to tune ribosome synthesis to cell state. Previous studies have implicated LARP1 in 40S- or 80S-ribosome complexes that are thought to repress and stabilize TOPs. However, a molecular understanding of how LARP1 and TOPs interact with ... More
16 total citations

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