SimplyBlue™ SafeStain
SimplyBlue™ SafeStain
SimplyBlue™ SafeStain
SimplyBlue™ SafeStain
SimplyBlue™ SafeStain
Invitrogen™

SimplyBlue™ SafeStain

Green features
SimplyBlue Safe Stain 是一种快速、灵敏且安全的即用型考马斯 G-250 染色剂,用于在聚丙烯酰胺凝胶和 PVDF 干膜上可视化蛋白条带。这是完全无害的,且不需要甲醇或乙酸固定剂或脱色液了解更多信息
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货号数量
LC60601 L
LC60653.5 L
货号 LC6060
价格(CNY)
1,994.00
Online Exclusive
Ends: 31-Dec-2025
2,850.00
共减 856.00 (30%)
Each
-
添加至购物车
数量:
1 L
Recurring order eligible. Learn more »
请求批量或定制报价
价格(CNY)
1,994.00
Online Exclusive
Ends: 31-Dec-2025
2,850.00
共减 856.00 (30%)
Each
添加至购物车
Ask our AI about this Product
SimplyBlue Safe Stain 是一种快速、灵敏且安全的即用型考马斯 G-250 染色剂,用于在聚丙烯酰胺凝胶和 PVDF 干膜上可视化蛋白条带。这是完全无害的,且不需要甲醇或乙酸固定剂或脱色液。消除了危险接触和不良气味的风险,营造更健康的实验室环境。

比较所有考马斯染色剂›

易于使用的方案
SimplyBlue SafeStain 方案易于执行,并且可以在不到三个小时的时间内完成。对于更高的速度,可在 12 分钟内完成简单的微波程序。虽然不是必需的,但可使用去离子水进行脱色以实现较高灵敏度,尤其是在进行下游分析时,如质谱分析或者需要水晶般透明的背景时。
仅供科研使用。不可用于诊断程序。
规格
检测定位凝胶内检测
检测方法比色法
环保功能危害更小
产品线SimplyBlue™
产品类型安全蛋白染色剂
数量1 L
有效期每 6 个月
靶标分子蛋白质
颜色Blue
标签或染料Coomassie
Unit SizeEach
内容与储存
SimplyBlue™ SafeStain 以 1X 即用型染色试剂的形式提供。在室温下储存。如果储存恰当,可以保证稳定6个月。本品不会产生危险品费用。
iBlot 3 Western Blot Transfer System

Introducing iBlot 3 Western Blot Transfer System

Featuring higher throughput and built-in cooling for consistent protein transfer
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常见问题解答 (FAQ)

It is not recommended because the background will be too high. Better alternatives include: 1) Invitrogen Reversible Membrane Protein Stain Kit (Cat. No. IB7710).

2) Coomassie (non-colloidal) staining: stain in 0.1% Coomassie Blue R-250 in 50% methanol for 5 min and destain with several changes of 50% methanol and 10% acetic acid. Rinse with several changes of water, air dry and store for up to 12 months at -20°C. Sensitivity is approximately at the 50-100 ng level.

3) Use SimplyBlue SafeStain (Cat. No. LC6060). The SimplyBlue SafeStain manual has the protocol for staining PVDF membranes, but it is not recommended for nitrocellulose because of high background.

4) Amido Black: same as Coomassie but less sensitive.

5) Ponceau S: same as Coomassie but less sensitive.

6) UV transillumination: place membrane on filter paper after blot is finished and allow to dry at room temperature for about 10 min. Rewet in 20% methanol and view the blot in front of white light while it is still wet; the bands will look more translucent than the membrane. If the bands disappear as the membranes dries, rewet again.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Coomassie G-250 will give a sharp dye front with both NuPAGE MES and MOPS Running Buffers and is therefore used as the tracking dye in the NuPAGE LDS Sample Buffer.

Bromophenol blue runs more slowly than some peptides with the NuPAGE MES Running Buffer system.

Coomassie G-250 migrates much closer to the moving ion front than bromophenol blue, ensuring that small peptides will not be run too far (e.g., off the gel).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

The Colloidal Blue Staining Kit (Cat. No. LC6025) is best for quantitation by densitometry. You can also use SimplyBlue SafeStain for this application.

The great advantage of SimplyBlue SafeStain is that it is very easy to use and safe.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Check the cap on the bottle. If the bottles are not tightly sealed, the alcohol can evaporate from the stain causing substantial decrease in stain sensitivity.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

After staining with SimplyBlue SafeStain, use deionized water for the less strongly retained protein bands on the PVDF membrane.

Increasing methanol or ethanol concentrations up to 70% should destain any remaining bands. You can leave the membrane in the destain indefinitely.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

引用和文献 (5)

引用和文献
Abstract
Enzymatic hydrolysis of pyridoxine-5'-beta-D-glucoside is catalyzed by intestinal lactase-phlorizin hydrolase.
Authors: Mackey Amy D; Henderson George N; Gregory Jesse F 3rd;
Journal:J Biol Chem
PubMed ID:12023280
'An obligatory step in the mammalian nutritional utilization of pyridoxine-5''-beta-D-glucoside (PNG) is the intestinal hydrolysis of its beta-glucosidic bond that releases pyridoxine (PN). This laboratory previously reported the purification and partial characterization of a novel cytosolic enzyme, designated PNG hydrolase, which hydrolyzed PNG. An investigation of the subcellular distribution of ... More
2F3 monoclonal antibody recognizes the O26 O-antigen moiety of the lipopolysaccharide of enterohemorrhagic Escherichia coli strain 4276.
Authors:Szalo IM, Taminiau B, Goffaux F, Pirson V, McCappin J, Ball HJ, Mainil JG,
Journal:Clin Diagn Lab Immunol
PubMed ID:15138178
'Enterohemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli (EPEC) organisms are groups of pathogenic strains whose infections are characterized by a typical lesion of enterocyte attachment and effacement. They are involved in enteric diseases both in humans and in animals, and EHEC strains can be responsible for hemolytic uremic syndrome ... More
Selective fluorescent labeling of S-nitrosothiols (S-FLOS): a novel method for studying S-nitrosation.
Authors:Santhanam L, Gucek M, Brown TR, Mansharamani M, Ryoo S, Lemmon CA, Romer L, Shoukas AA, Berkowitz DE, Cole RN,
Journal:Nitric Oxide
PubMed ID:18706513
'Protein S-nitrosation is a reversible post-translation modification critical for redox-sensitive cell signaling that is typically studied using the Biotin Switch method. This method and subsequent modifications usually require avidin binding or Western blot analysis to detect biotin labeled proteins. We describe here a modification of the Biotin Switch assay that ... More
Vascular endothelial growth factor-C and C-C chemokine receptor 7 in tumor cell-lymphatic cross-talk promote invasive phenotype.
Authors:Issa A, Le TX, Shoushtari AN, Shields JD, Swartz MA,
Journal:Cancer Res
PubMed ID:19118020
'Most carcinomas spread to distant sites through lymphatic vessels. Several preclinical and clinical studies have shown a positive correlation between the incidence of lymph node metastasis and secretion of the lymphatic growth factor vascular endothelial growth factor-C (VEGF-C) by tumor cells, suggesting tumor lymphangiogenesis as an escape mechanism. However, recent ... More
Yeast Expression and NMR Analysis of the Extracellular Domain of Muscle Nicotinic Acetylcholine Receptor alpha Subunit.
Authors: Yao Yun; Wang Junmei; Viroonchatapan Nitnara; Samson Avraham; Chill Jordan; Rothe Elizabeth; Anglister Jacob; Wang Zuo-Zhong;
Journal:J Biol Chem
PubMed ID:11812776
The alpha subunit of the nicotinic acetylcholine receptor (AChR) from Torpedo electric organ and mammalian muscle contains high affinity binding sites for alpha-bungarotoxin and for autoimmune antibodies in sera of patients with myasthenia gravis. To obtain sufficient materials for structural studies of the receptor-ligand complexes, we have expressed part of ... More
5 total citations

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